AAT Bioquest

mFluor™ Violet 480 SE

Product Image
Product Image
Gallery Image 1
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Spectral properties
Extinction coefficient (cm -1 M -1)40,000
Excitation (nm)404
Emission (nm)475
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
mFluor™ Violet 480 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 480 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. These dyes have fluorescence excitation and emission maxima of ~405 nm and ~480 nm respectively. These spectral characteristics make them a unique color for flow cytometry application. mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 350 nm, 405 nm, 488 nm and 633 nm). mFluor™ Violet dyes are optimized to be excited with a violet laser at 405 nm. AAT Bioquest offers the largest collection of fluorescent dyes that are excited by violet laser.


Open in Advanced Spectrum Viewer

Spectral properties

Extinction coefficient (cm -1 M -1)40,000
Excitation (nm)404
Emission (nm)475

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 450 SE4064453500010.8110.3380.078
mFluor™ Violet 510 SE4125052500010.8610.4640.366
mFluor™ Violet 540 SE4025351800010.2111.3260.543
mFluor™ Violet 500 SE4105012500010.8110.7690.365
mFluor™ Violet 610 SE4216129000010.310.5320.66
mFluor™ Violet 550 SE5275509000010.3110.4740.306
mFluor™ Violet 505 SE3935044000010.4510.8880.403
mFluor™ Violet 590 SE5645919000010.2210.6320.329
mFluor™ Violet 545 SE3935432000010.1511.080.496
mFluor™ Violet 530 SE393543200001---
Show More (1)



View all 3 references: Citation Explorer
A miniaturized, low-cost lens tube based laser-induced fluorescence detection system for automated microfluidic analysis of primary amines.
Authors: Duca, Zachary A and Speller, Nicholas C and Cato, Michael E and Morbioli, Giorgio Gianini and Stockton, Amanda M
Journal: Talanta (2022): 123227
Development of dual-color total internal reflection fluorescence biosensor for simultaneous quantitation of two small molecules and their affinity constants with antibodies.
Authors: Song, Dan and Yang, Rong and Wang, Hongliang and Fang, Sunyan and Liu, Yanping and Long, Feng and Zhu, Anna
Journal: Biosensors & bioelectronics (2019): 824-830
A novel dual-color total internal reflection fluorescence detecting platform using compact optical structure and silicon-based photodetector.
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Liu, Jiayao and Xu, Wenjuan and Long, Feng and Zhu, Anna
Journal: Talanta (2019): 78-84