mFluor™ Violet 480 SE
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 1.23 |
| Correction Factor (280 nm) | 0.6570 |
| Extinction coefficient (cm -1 M -1) | 40,000 |
| Excitation (nm) | 404 |
| Emission (nm) | 475 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
See also: mFluor™ Dyes and Kits
Correction Factor (260 nm) 1.23 | Correction Factor (280 nm) 0.6570 | Extinction coefficient (cm -1 M -1) 40,000 | Excitation (nm) 404 | Emission (nm) 475 |
mFluor™ Violet 480 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 480 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. These dyes have fluorescence excitation and emission maxima of ~405 nm and ~480 nm respectively. These spectral characteristics make them a unique color for flow cytometry application. mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 350 nm, 405 nm, 488 nm and 633 nm). mFluor™ Violet dyes are optimized to be excited with a violet laser at 405 nm. AAT Bioquest offers the largest collection of fluorescent dyes that are excited by violet laser.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 1.23 |
| Correction Factor (280 nm) | 0.6570 |
| Extinction coefficient (cm -1 M -1) | 40,000 |
| Excitation (nm) | 404 |
| Emission (nm) | 475 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| mFluor™ Violet 450 SE | 406 | 445 | 350001 | 0.811 | 0.338 | 0.078 |
| mFluor™ Violet 510 SE | 412 | 505 | 250001 | 0.861 | 0.464 | 0.366 |
| mFluor™ Violet 540 SE | 402 | 535 | 180001 | 0.211 | 1.326 | 0.543 |
| mFluor™ Violet 500 SE | 410 | 501 | 250001 | 0.811 | 0.769 | 0.365 |
| mFluor™ Violet 610 SE | 421 | 612 | 900001 | 0.31 | 0.532 | 0.66 |
| mFluor™ Violet 550 SE | 527 | 550 | 900001 | 0.311 | 0.474 | 0.306 |
| mFluor™ Violet 505 SE | 393 | 504 | 400001 | 0.451 | 0.888 | 0.403 |
| mFluor™ Violet 590 SE | 564 | 591 | 900001 | 0.221 | 0.632 | 0.329 |
| mFluor™ Violet 545 SE | 393 | 543 | 200001 | 0.151 | 1.08 | 0.496 |
Show More (1) | ||||||
Images
Figure 1. Figure 1. Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with CD4-mFluor™ Violet 480 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor™ Violet 480 specific V4-A channel.
Figure 2. mFluor™ Violet 480 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 480 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. These dyes have fluorescence excitation and emission maxima of ~405 nm and ~480 nm respectively. These spectral characteristics make them a unique color for flow cytometry application.
References
View all 3 references: Citation Explorer
A miniaturized, low-cost lens tube based laser-induced fluorescence detection system for automated microfluidic analysis of primary amines.
Authors: Duca, Zachary A and Speller, Nicholas C and Cato, Michael E and Morbioli, Giorgio Gianini and Stockton, Amanda M
Journal: Talanta (2022): 123227
Authors: Duca, Zachary A and Speller, Nicholas C and Cato, Michael E and Morbioli, Giorgio Gianini and Stockton, Amanda M
Journal: Talanta (2022): 123227
Development of dual-color total internal reflection fluorescence biosensor for simultaneous quantitation of two small molecules and their affinity constants with antibodies.
Authors: Song, Dan and Yang, Rong and Wang, Hongliang and Fang, Sunyan and Liu, Yanping and Long, Feng and Zhu, Anna
Journal: Biosensors & bioelectronics (2019): 824-830
Authors: Song, Dan and Yang, Rong and Wang, Hongliang and Fang, Sunyan and Liu, Yanping and Long, Feng and Zhu, Anna
Journal: Biosensors & bioelectronics (2019): 824-830
A novel dual-color total internal reflection fluorescence detecting platform using compact optical structure and silicon-based photodetector.
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Liu, Jiayao and Xu, Wenjuan and Long, Feng and Zhu, Anna
Journal: Talanta (2019): 78-84
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Liu, Jiayao and Xu, Wenjuan and Long, Feng and Zhu, Anna
Journal: Talanta (2019): 78-84