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United States of America
Telephone:1-408-733-1055
Fax:1-408-733-1304
Email:sales@aatbio.com
Purchase Order:sales@aatbio.com

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mFluor™ Violet 480 SE

mFluor™ Violet 480 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 480 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. These dyes have fluorescence excitation and emission maxima of ~405 nm and ~480 nm respectively. These spectral characteristics make them a unique color for flow cytometry application. mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 350 nm, 405 nm, 488 nm and 633 nm). mFluor™ Violet dyes are optimized to be excited with a violet laser at 405 nm. AAT Bioquest offers the largest collection of fluorescent dyes that are excited by violet laser.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 450 SE4064453500010.8110.3380.078
mFluor™ Violet 510 SE4125052500010.8610.4640.366
mFluor™ Violet 540 SE4025351800010.2111.3260.543
mFluor™ Violet 500 SE4105012500010.8110.7690.365
mFluor™ Violet 610 SE5946129000010.310.5320.66
mFluor™ Violet 550 SE5275509000010.3110.4740.306
mFluor™ Violet 505 SE3935044000010.4510.8880.403
mFluor™ Violet 590 SE5645919000010.2210.6320.329
mFluor™ Violet 545 SE3935432000010.1511.080.496
mFluor™ Violet 530 SE393543200001---
Show More (1)

References

View all 3 references: Citation Explorer
A miniaturized, low-cost lens tube based laser-induced fluorescence detection system for automated microfluidic analysis of primary amines.
Authors: Duca, Zachary A and Speller, Nicholas C and Cato, Michael E and Morbioli, Giorgio Gianini and Stockton, Amanda M
Journal: Talanta (2022): 123227
A novel dual-color total internal reflection fluorescence detecting platform using compact optical structure and silicon-based photodetector.
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Liu, Jiayao and Xu, Wenjuan and Long, Feng and Zhu, Anna
Journal: Talanta (2019): 78-84
Development of dual-color total internal reflection fluorescence biosensor for simultaneous quantitation of two small molecules and their affinity constants with antibodies.
Authors: Song, Dan and Yang, Rong and Wang, Hongliang and Fang, Sunyan and Liu, Yanping and Long, Feng and Zhu, Anna
Journal: Biosensors & bioelectronics (2019): 824-830
Page updated on September 13, 2024

Ordering information

Price
Unit size
Catalog Number1158
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

676.78

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

1.23

Correction Factor (280 nm)

0.6570

Extinction coefficient (cm -1 M -1)

40,000

Excitation (nm)

404

Emission (nm)

475

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
Figure 1. Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with CD4-mFluor™ Violet 480 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor™ Violet 480 specific V4-A channel.
Figure 1. Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with CD4-mFluor™ Violet 480 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor™ Violet 480 specific V4-A channel.
Figure 1. Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with CD4-mFluor™ Violet 480 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor™ Violet 480 specific V4-A channel.
Gallery Image 2

Documents

pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
mFluor™ Dyes and Kits
mFluor™ Blue 570 SE
mFluor™ Green 620 SE
mFluor™ Yellow 630 SE