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mFluor™ Violet 480 SE

mFluor™ Violet 480 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 480 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. These dyes have fluorescence excitation and emission maxima of ~405 nm and ~480 nm respectively. These spectral characteristics make them a unique color for flow cytometry application. mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 350 nm, 405 nm, 488 nm and 633 nm). mFluor™ Violet dyes are optimized to be excited with a violet laser at 405 nm. AAT Bioquest offers the largest collection of fluorescent dyes that are excited by violet laser.

Figure 1. Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with CD4-mFluor™ Violet 480 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor™ Violet 480 specific V4-A channel.
Figure 1. Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with CD4-mFluor™ Violet 480 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor™ Violet 480 specific V4-A channel.
CatalogSize
Price
Quantity
11581 mg
Price
 
Physical properties

Molecular weight676.78
SolventDMSO
Spectral properties

Correction factor (260 nm)1.23
Correction factor (280 nm)0.6570
Extinction coefficient (cm -1 M -1)
40,000
Excitation (nm)404
Emission (nm)475
Quantum yield
0.625
1
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Page updated on October 21, 2025