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ReadiLink™ Rapid FITC Antibody Labeling Kit
Microscale Optimized for Labeling 50 μg Antibody Per Reaction
FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates. However, most of commercial FITC labeling kits require intensive hands-on time. This FITC ReadiLink™ labeling kit essentially only requires 2 simple mixing steps without a column purification needed. The kit provides all the essential components for labeling ~2x50 ug antibody. Each of the two vials of FITC dye provided in the kit is optimized for labeling ~50 µg antibody. This FITC protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa).

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by AAT’s FITC goat anti-mouse IgG conjugate (Green, Left) or Jackson’s FITC goat anti-mouse IgG conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).
HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by AAT’s FITC goat anti-mouse IgG conjugate (Green, Left) or Jackson’s FITC goat anti-mouse IgG conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).
CatalogSize
Price
Quantity
12992 Labelings
Price
 
Spectral properties

Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)491
Emission (nm)516
Quantum yield
0.92
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 9, 2025