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ReadiLink™ xtra Rapid Cy5 Antibody Labeling Kit
BSA-Compatible
ReadiLink™ xtra rapid antibody labeling kits require essentially only 2 simple mixing steps without a column purification needed. Specially formulated Cy5 used in this ReadiLink™ kit is quite stable and shows good reactivity and selectivity with antibodies. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of specially formulated Cy5 dye provided in the kit is optimized for labeling ~50 µg antibody. ReadiLink™ xtra Cy5 rapid antibody labeling kit provides a convenient and robust method to label monoclonal and polyclonal antibodies with the red fluorescent Cy5 fluorophore. Cy5 is one of the most used fluorophores for labeling antibodies.

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ xtra Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

Immunofluorescence staining of tubulin in HeLa cells. HeLa cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 and blocked. Cells were then incubated with mouse anti-tubulin monoclonal antibody and stained with a goat anti-mouse IgG labeled using the ReadiLink™ xtra Rapid Cy5 Antibody Labeling Kit (Cat No. 1972). The cell nuclei were counterstained using DAPI (Cat No. 17507).
Immunofluorescence staining of tubulin in HeLa cells. HeLa cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 and blocked. Cells were then incubated with mouse anti-tubulin monoclonal antibody and stained with a goat anti-mouse IgG labeled using the ReadiLink™ xtra Rapid Cy5 Antibody Labeling Kit (Cat No. 1972). The cell nuclei were counterstained using DAPI (Cat No. 17507).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
19722 Labelings
Price
 
Spectral properties
Correction factor (260 nm)0.02
Correction factor (280 nm)0.03
Correction factor (482 nm)0.009
Correction factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)651
Emission (nm)670
Quantum yield
0.27
1
, 
0.4
2
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 22, 2025