Calculator
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 313.28 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.25 |
| Correction Factor (280 nm) | 0.19 |
| Extinction coefficient (cm -1 M -1) | 19000 |
| Excitation (nm) | 343 |
| Emission (nm) | 441 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| Overview |  SDSProtocol |
See also: Antibody and Protein Labeling
Molecular weight 313.28 | Correction Factor (260 nm) 0.25 | Correction Factor (280 nm) 0.19 | Extinction coefficient (cm -1 M -1) 19000 | Excitation (nm) 343 | Emission (nm) 441 |
XFD350 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 350 (Alexa Fluor® is the trademark of ThermoFisher). XFD350 acid has the same molecule as Alexa Fluor® 350 acid. It is a bright blue-fluorescent dye optimal for use with the UV laser line at 350 nm. XFD350 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD350 acid is the most economic amine-reactive form for conjugating XFD350 fluorophore to a peptide.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD350 acid *Same Structure to Alexa Fluor™ 350 acid* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 319.203 µL | 1.596 mL | 3.192 mL | 15.96 mL | 31.92 mL |
| 5 mM | 63.841 µL | 319.203 µL | 638.407 µL | 3.192 mL | 6.384 mL |
| 10 mM | 31.92 µL | 159.602 µL | 319.203 µL | 1.596 mL | 3.192 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.25 |
| Correction Factor (280 nm) | 0.19 |
| Extinction coefficient (cm -1 M -1) | 19000 |
| Excitation (nm) | 343 |
| Emission (nm) | 441 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
| XFD532 acid *Same Structure to Alexa Fluor™ 532 acid* | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| XFD488 acid *Same Structure to Alexa Fluor™ 488 acid* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD546 acid *Same Structure to Alexa Fluor™ 546 acid* | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
| XFD568 acid *Same Structure to Alexa Fluor™ 568 acid* | 579 | 603 | 91300 | 0.691 | 0.45 | 0.46 |
| XFD514 acid | 518 | 543 | 80000 | - | 0.31 | 0.18 |
| XFD555 acid | 553 | 568 | 155000 | 0.11 | 0.08 | 0.08 |
| XFD647 acid | 650 | 671 | 270000 | 0.331 | - | 0.03 |
| XFD750 acid | 752 | 776 | 290000 | 0.121 | - | 0.04 |
Show More (13) | ||||||
Citations
View all 2 citations: Citation Explorer
A combined solvatochromic shift and TDDFT study probing solute-solvent interactions of blue fluorescent Alexa Fluor 350 dye: Evaluation of ground and excited state dipole moments
Authors: Patil, M. K., Kotresh, M. G., Inamdar, S. R.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152
Authors: Patil, M. K., Kotresh, M. G., Inamdar, S. R.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152
Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy
Authors: Fan, S., Webb, J. E. A., Yang, Y., Nieves, D. J., Goncales, V. R., Tran, J., Hilzenrat, G., Kahram, M., Tilley, R. D., Gaus, K., Gooding, J. J.
Journal: Angew Chem Int Ed Engl (2019): 14495-14498
Authors: Fan, S., Webb, J. E. A., Yang, Y., Nieves, D. J., Goncales, V. R., Tran, J., Hilzenrat, G., Kahram, M., Tilley, R. D., Gaus, K., Gooding, J. J.
Journal: Angew Chem Int Ed Engl (2019): 14495-14498
References
View all 1 references: Citation Explorer
Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates
Authors: Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugl and RP., undefined
Journal: J Histochem Cytochem (2003): 1699
Authors: Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugl and RP., undefined
Journal: J Histochem Cytochem (2003): 1699
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides