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XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*

XFD546, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 546 (ThermoFisher). It exhibits bright orange fluorescence and is readily excited by laser lines at 488 nm or 532 nm, making it highly suitable for applications such as fluorescence microscopy and flow cytometry. XFD546 demonstrates excellent aqueous solubility and pH-insensitivity over a broad range (pH 4–10), ensuring stable signal generation under varying experimental conditions. Additionally, the dye enables high molar ratio conjugation to proteins with minimal fluorescence quenching, facilitating the generation of brighter conjugates for enhanced detection sensitivity. With its high fluorescence quantum yield and superior photostability, XFD546 is particularly advantageous for detecting low-abundance biological targets, providing researchers with improved sensitivity and precision in quantitative fluorescence-based assays.

XFD546 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing XFD546 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*534553810000.6110.240.09
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*499520710000.9210.300.11
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*34344119000-0.250.19
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*579603913000.6910.450.46
XFD514 acid51854380000-0.310.18
XFD555 acid5535681550000.110.080.08
XFD647 acid6506712700000.331-0.03
XFD750 acid7527762900000.121-0.04
XFD700 acid6967192050000.251-0.07
XFD405 acid40142135,000-0.230.70
XFD430 acid43254015,000--0.28
XFD546 amine5615721120000.7910.210.12
XFD546 azide5615721120000.7910.210.12
XFD546 alkyne5615721120000.7910.210.12
XFD546 TCO5615721120000.7910.210.12
XFD546 Tetrazine5615721120000.7910.210.12
XFD594 acid590618900000.6610.430.56
ICG acid789813230000--0.076
Cy3NS acid *CAS 1032678-01-5*55556915000010.1510.070.073
Cy5NS acid65167025000010.271, 0.420.020.03
Cy7NS acid7567792500000.30.050.036
AMCA acid *CAS#: 106562-32-7*34643419000--0.153
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7*3364555900--0.107
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]3364555900--0.107
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*-----0.516
BCECF acid *CAS#: 85138-49-4*50452790000---
Cy3B acid56057112000010.5810.0480.069
QXY7 acid [equivalent to QSY-7 acid]--900001--0.22
OG514 acid [equivalent to Oregon Green® 514 carboxylic acid]51353385000--0.19
Show More (20)

Citations

View all 2 citations: Citation Explorer
Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy
Authors: Fan, S., Webb, J. E. A., Yang, Y., Nieves, D. J., Goncales, V. R., Tran, J., Hilzenrat, G., Kahram, M., Tilley, R. D., Gaus, K., Gooding, J. J.
Journal: Angew Chem Int Ed Engl (2019): 14495-14498
A combined solvatochromic shift and TDDFT study probing solute-solvent interactions of blue fluorescent Alexa Fluor 350 dye: Evaluation of ground and excited state dipole moments
Authors: Patil, M. K., Kotresh, M. G., Inamdar, S. R.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152

References

View all 1 references: Citation Explorer
Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates
Authors: Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugl and RP., undefined
Journal: J Histochem Cytochem (2003): 1699
Page updated on October 9, 2024

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Catalog Number1791
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Physical properties

Molecular weight

1264.91

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.12

Extinction coefficient (cm -1 M -1)

112000

Excitation (nm)

561

Emission (nm)

572

Quantum yield

0.791

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501