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XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*

Ordering information
Price ()
Catalog Number1791
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1264.91
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)112000
Excitation (nm)561
Emission (nm)572
Quantum yield0.791
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Related products
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide*
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne*
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*
XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide*
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester*
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester*
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*
XFD350 C5 Maleimide *Same Structure to Alexa Fluor™ 350 C5 Maleimide*
XFD532 C5 Maleimide *Same Structure to Alexa Fluor™ 532 C5 Maleimide*
XFD594 C5 Maleimide *Same Structure to Alexa Fluor™ 594 C5 Maleimide*
XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine*
XFD350 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*
XFD488 tyramide reagent *Same Structure to Alexa Fluor™ 488 tyramide*
XFD546 tyramide reagent *Same Structure to Alexa Fluor™ 546 tyramide*
XFD594 tyramide reagent *Same Structure to Alexa Fluor™ 594 tyramide*
XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488*
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester*
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester*
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester*
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester*
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester*
XFD647 maleimide *C2, XFD647 Same Structure to Alexa Fluor™ 647*
XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester*
XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester*
XFD350 tyramide reagent *Same Structure to Alexa Fluor™ 350 tyramide*
XFD568 tyramide reagent *Same Structure to Alexa Fluor™ 568 tyramide*
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*
XFD™488-dUTP *1 mM in Tris Buffer (pH 7.5)*
XFD514 NHS Ester *Same Structure to Alexa Fluor™ 514 NHS Ester*
XFD514 tyramide
XFD532 tyramide
XFD647 Azide
XFD647 Alkyne
XFD488 TCO
Show More (48)

OverviewpdfSDSpdfProtocol


Molecular weight
1264.91
Extinction coefficient (cm -1 M -1)
112000
Excitation (nm)
561
Emission (nm)
572
Quantum yield
0.791
XFD546 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 546 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright red-fluorescent dye optimal for use with the yellow laser line at 546 nm. XFD546 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD546 acid is the most economic amine-reactive form for conjugating XFD546 fluorophore to a peptide.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD546 acid *Same Structure to Alexa Fluor™ 546 acid* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM79.057 µL395.285 µL790.57 µL3.953 mL7.906 mL
5 mM15.811 µL79.057 µL158.114 µL790.57 µL1.581 mL
10 mM7.906 µL39.529 µL79.057 µL395.285 µL790.57 µL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)112000
Excitation (nm)561
Emission (nm)572
Quantum yield0.791

Citations


View all 2 citations: Citation Explorer
A combined solvatochromic shift and TDDFT study probing solute-solvent interactions of blue fluorescent Alexa Fluor 350 dye: Evaluation of ground and excited state dipole moments
Authors: Patil, M. K., Kotresh, M. G., Inamdar, S. R.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152
Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy
Authors: Fan, S., Webb, J. E. A., Yang, Y., Nieves, D. J., Goncales, V. R., Tran, J., Hilzenrat, G., Kahram, M., Tilley, R. D., Gaus, K., Gooding, J. J.
Journal: Angew Chem Int Ed Engl (2019): 14495-14498

References


View all 1 references: Citation Explorer
Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates
Authors: Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugl and RP., undefined
Journal: J Histochem Cytochem (2003): 1699