XFD488 Anti-human CD40 Antibody *G28.5, XFD488 Same Structure to Alexa Fluor™ 488*

G28.5 is an anti-human monoclonal antibody that targets the CD40 antigen. CD40 (also known as Bp50) is a 48 kD transmembrane protein that is expressed on the surface of cells like macrophages, dendritic cells and stem cells. CD40 is a component of key cellular pathways, in particular, the tumor necrosis factor-mediated signaling pathway, immune response-regulating cell surface receptor signaling pathway and CD40 signaling pathway. Also, in many organisms, it upregulates interleukin-12 production, is a positive regulator of isotype switching to IgG isotypes and is a promoter of protein phosphorylation. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like CD40L, CD154 and TRAP. CD40 is a very popular antibody target, with over 30000 publications in the last decade. CD40 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of neuroscience. This antibody was purified through affinity chromatography and conjugated to XFD488 (ex/em = 499/520 nm). XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). It is compatible with the 488 nm laser and 525/45 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte Advanteon).