XFD488 Anti-human CD53 Antibody *HI29, XFD488 Same Structure to Alexa Fluor™ 488*

HI29 is an anti-human monoclonal antibody that targets the CD53 antigen. CD53 (sometimes referred to as Tetraspanin-25, MOX44 or OX44) is a 35 - 42 kD member of the tetraspan family that is found on the surface of cells such as NK cells. In some organisms, CD53 enhances myoblast fusion, and is associated with a variety of biologically interesting macromolecules/ligands, in particular, VLA-4, Integrins and HLA-DR. CD53 is a relatively rare antibody target, with fewer than 600 publications in the last decade. Even still, CD53 has a variety of applications in costimulatory molecules research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to XFD488 (ex/em = 499/520 nm). XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). It is compatible with the 488 nm laser and 533/30 nm bandpass filter (for example, as in the BD Accuri™ C6 Plus).