XFD555 Anti-human CD97 Antibody *MEM-180, XFD555 Same Structure to Alexa Fluor™ 555*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (260 nm) | 0.08 |
Correction Factor (280 nm) | 0.08 |
Extinction coefficient (cm -1 M -1) | 150000 |
Excitation (nm) | 553 |
Emission (nm) | 568 |
Quantum yield | 0.11 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names BL-KDDF12 | Clone MEM-180 | Host mouse | Isotype Mouse IgG1 | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Correction Factor (260 nm) 0.08 | Correction Factor (280 nm) 0.08 | Extinction coefficient (cm -1 M -1) 150000 | Excitation (nm) 553 | Emission (nm) 568 | Quantum yield 0.11 |
The MEM-180 monoclonal antibody binds with human CD97, a 74 kD multi-pass membrane protein typically expressed on the surface of macrophages, dendritic cells, granulocytes and monocytes. CD97 is involved with essential cellular pathways, namely, the G protein-coupled receptor signaling pathway and cell surface receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands such as CD55 (DAF). CD97 is a relatively rare antibody target, with fewer than 600 publications in the last decade. Even still, CD97 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to XFD555 (ex/em = 553/568 nm). XFD555 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 555 (Alexa Fluor® is the trademark of ThermoFisher). It is compatible with the 561 nm laser and 577/35 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.08 |
Correction Factor (280 nm) | 0.08 |
Extinction coefficient (cm -1 M -1) | 150000 |
Excitation (nm) | 553 |
Emission (nm) | 568 |
Quantum yield | 0.11 |
Antibody Finder
Select Your CD Antibody | |
Select Your Clone | |
Select Your Conjugate |
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd97 antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?