XFD647 Anti-human CD111 Antibody *R1.302, XFD647 Same Structure to Alexa Fluor™ 647*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (260 nm) | 0.00 |
Correction Factor (280 nm) | 0.03 |
Extinction coefficient (cm -1 M -1) | 239000 |
Excitation (nm) | 650 |
Emission (nm) | 671 |
Quantum yield | 0.331 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names Nectin-1; HVEC; PVRL1; PRR1 | Clone R1.302 | Host mouse | Isotype Mouse IgG1 kappa | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Correction Factor (260 nm) 0.00 | Correction Factor (280 nm) 0.03 | Extinction coefficient (cm -1 M -1) 239000 | Excitation (nm) 650 | Emission (nm) 671 | Quantum yield 0.331 |
R1.302 is an anti-human monoclonal antibody that forms an immune complex with the CD111 antigen. CD111 (also known as PVRL1 or Nectin-1) is a 75 kD single-pass type I membrane protein that is found on the surface of cells like erythrocytes, endothelial cells, epithelial cells, stem cells and macrophages. CD111 has been thought to be involved with critical biological processes like cell-cell adhesion, particularly homophilic cell adhesion via plasma membrane adhesion molecules, and is associated with a variety of biologically interesting macromolecules/ligands, for instance, nectin3 and afadin gd. CD111 is a relatively rare antibody target, with fewer than 90 publications in the last decade. Even still, CD111 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of neuroscience. This antibody was purified through affinity chromatography and conjugated to XFD647 (ex/em = 650/671 nm). XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher). It is compatible with the 642 nm laser and 702/85 nm bandpass filter (for example, as in the Luminex Amnis FlowSight).
Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.00 |
Correction Factor (280 nm) | 0.03 |
Extinction coefficient (cm -1 M -1) | 239000 |
Excitation (nm) | 650 |
Emission (nm) | 671 |
Quantum yield | 0.331 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd111 antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?