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AAT Bioquest

XFD680 Anti-human CD235 Antibody *HI264, XFD680 Same Structure to Alexa Fluor™ 680*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Correction Factor (260 nm)0.00
Correction Factor (280 nm)0.05
Extinction coefficient (cm -1 M -1)184000
Excitation (nm)681
Emission (nm)704
Quantum yield0.361
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
GYPA; Sialoglycoprotein alpha; PAS-2
Clone
HI264
Host
mouse
Isotype
mouse IgG2a
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Correction Factor (260 nm)
0.00
Correction Factor (280 nm)
0.05
Extinction coefficient (cm -1 M -1)
184000
Excitation (nm)
681
Emission (nm)
704
Quantum yield
0.361
HI264 is an anti-human monoclonal antibody that forms an immune complex with the CD235a antigen. CD235a (sometimes called MN sialoglycoprotein or MNS blood group) is a 10 kD single-pass type i membrane protein that is expressed on the surface of cells like erythrocytes. CD235 is associated with a variety of biologically interesting macromolecules/ligands, namely, Plasmodium falci- parum erythrocyte binding antigen EBA-175, CD170 and Influenza virus. CD235 is a relatively rare antibody target, with fewer than FAKE6000FAKE publications in the last decade. Even still, CD235a is essential for cell adhesion and immunology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to XFD680 (ex/em = 681/704 nm). XFD680 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 680 (Alexa Fluor® is the trademark of ThermoFisher). It is compatible with the 642 nm laser and 664/20 nm bandpass filter (for example, as in the Luminex Guava easyCyte).

Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.00
Correction Factor (280 nm)0.05
Extinction coefficient (cm -1 M -1)184000
Excitation (nm)681
Emission (nm)704
Quantum yield0.361

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd235a antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.