XFD750 Anti-human CD279 Antibody 2E7, XFD750 Same Structure to Alexa Fluor™ 750

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Spectral properties

Correction Factor (260 nm)	0.00
Correction Factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	240000
Excitation (nm)	752
Emission (nm)	776
Quantum yield	0.12¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Overview SDS Protocol

Other names

PD1

Clone

2E7

Host

mouse

Reactivity

human

Application

Flow Cytometry (FACS); Fluorescence Imaging

Correction Factor (260 nm)

0.00

Correction Factor (280 nm)

0.04

Extinction coefficient (cm ^-1 M ^-1)

240000

Excitation (nm)

752

Emission (nm)

776

Quantum yield

0.12¹

2E7 is an anti-human monoclonal antibody that targets the CD279 antigen. CD279 (sometimes referred to as Programmed Death-1 or PD-1) is a 50 - 55 kD glycoprotein that is found on the surface of cells like B cells and T cells. CD279 is associated with a variety of biologically interesting macromolecules/ligands, namely, PDL1. CD279 is a relatively rare antibody target, with less than 1000 publications in the last decade. Even still, CD279 has been widely used in cancer biomarkers, immunology and inhibitory molecules research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to XFD750 (ex/em = 752/776 nm). XFD750 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 750 (Alexa Fluor® is the trademark of ThermoFisher).

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.00
Correction Factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	240000
Excitation (nm)	752
Emission (nm)	776
Quantum yield	0.12¹

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Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd279 antibody with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780