XFD750 Anti-human/ non-human primates CD89 Antibody *A59, XFD750 Same Structure to Alexa Fluor™ 750*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (260 nm) | 0.00 |
Correction Factor (280 nm) | 0.04 |
Extinction coefficient (cm -1 M -1) | 240000 |
Excitation (nm) | 752 |
Emission (nm) | 776 |
Quantum yield | 0.121 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names FCAR | Clone A59 | Host mouse | Isotype Mouse IgG1 kappa | Reactivity human; non-human primates | Application Flow Cytometry (FACS); Fluorescence Imaging | Correction Factor (260 nm) 0.00 | Correction Factor (280 nm) 0.04 | Extinction coefficient (cm -1 M -1) 240000 | Excitation (nm) 752 | Emission (nm) 776 | Quantum yield 0.121 |
The A59 monoclonal antibody binds with human/ non-human primates CD89, a 55 - 100 kD single-pass type I membrane protein often expressed on the surface of macrophages, eosinophils and neutrophils. CD89 acts in critical cellular pathways, in particular, the Fc receptor signaling pathway. Furthermore, in certain organisms, it upregulates neutrophil apoptotic process and is a promoter of oxidative stress-induced cell death. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands such as IgA2 and IgA1. CD89 is a relatively rare antibody target, with fewer than 500 publications in the last decade. Even still, CD89 is essential for immunology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to XFD750 (ex/em = 752/776 nm). XFD750 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 750 (Alexa Fluor® is the trademark of ThermoFisher).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.00 |
Correction Factor (280 nm) | 0.04 |
Extinction coefficient (cm -1 M -1) | 240000 |
Excitation (nm) | 752 |
Emission (nm) | 776 |
Quantum yield | 0.121 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd89 antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?