Brij® 35 Detergent

Type:	Non-ionic
Molecular Weight:	627
Molecular Formula:	C₁₂H₂₆O(OCH₂CH₂)₁₀
Absorbance:	(225 nm) 0.07
Critical Micelle Concentration (CMC)^(?):	0.09
Aggregation number	24-40
Aldehyde content	<100µM
Average micellar weight	48,000
Cloud Point	>100°C
Peroxide content (as H₂O₂)	<50µM
Applications:	Brij® 35 is used for protein extraction, cell permeabilization, and yeast spheroplast preparation.
Chemical Structure:

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Detergents ▲ ▼	CMC ▲ ▼
ASB-14	8
ASB-14-4	-
ASB-16	8
ASB-C7BzO	-
ASB-C8Ø	-
Big CHAP	3.4
Brij® 35	0.09
Brij® 58	0.007-0.077
C₁₂E₈	0.11
CHAPS	6-10
CHAPSO	8
CTAB	1
Cyclohexyl-n-hexyl-β-D-maltoside	0.56
DDMAB	4.3
Deoxy Big CHAP	1.1-1.4
Deoxycholate	4-8
Digitonin	0.2-0.73
HECAMEG	19.5
MEGA 10	6-7
MEGA 8	58
MEGA 9	19-25
n-Decanoylsucrose	2.5
n-Decyl-β-D-maltopyranoside	1.6
n-Dodecanoylsucrose	0.3
n-Dodecyl-β-D-glucopyranoside	0.13
n-Dodecyl-β-D-maltoside	0.1-0.6
n-Heptyl-β-D-glucopyranoside	79
n-Heptyl-β-D-thioglucopyranoside	30
n-Hexyl-β-D-glucopyranoside	250
n-Nonyl-β-D-glucopyranoside	6.5
n-Octanoylsucrose	24.4
n-Octyl-β-D-glucopyranoside	20-25
n-Octyl-β-D-maltopyranoside	23.4
n-Octyl-β-D-thioglucopyranoside	9
Nonidet® P-40 Substitute	0.05-0.3
Octyl β Glucoside	20-25
SDS	7-10
Sulfobetaine 3-10 (SB 3-10)	25-40
Sulfobetaine 3-12 (SB 3-12)	2-4
Sulfobetaine 3-14 (SB 3-14)	0.1-0.4
Triton® X-100	0.2
Triton® X-114	0.35
Tween® 20	0.06
Tween® 80	0.012
ZWITTERGENT® 3-10	25-40
ZWITTERGENT® 3-12	2-4
ZWITTERGENT® 3-14	0.1-0.4
ZWITTERGENT® 3-16	0.01-0.06

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