Triton® X-100 Detergent

Type	Non-ionic
Molecular Weight	647
Molecular Formula	C₃₄H₆₂O₁₁
Absorbance	(254 nm) 0.16
Critical Micelle Concentration (CMC) ^(?)	0.2
Aldehyde content	<100µM
Peroxide content (as H₂O₂)	<50µM
Aggregation number	100-155
Cloud Point	65°C
Average micellar weight	80,000
Applications	Triton® X-100 is a very common non ionic detergent that is used to solubilize membrane proteins during the isolation of membrane protein complexes. It has low amounts of aldehyde and peroxide concentrations, which reduce the effects of peroxidase and carbonyl compounds. These compounds negatively interact with membrane proteins.
Chemical Structure

List of detergents

Detergent Name	CMC	Type
ASB-14	8	Zwitterionic
ASB-14-4	-	Zwitterionic
ASB-16	8	Zwitterionic
ASB-C7BzO	-	Zwitterionic
ASB-C8ø	-	Zwitterionic
Big CHAP	3.4	Non-ionic
Brij® 35	0.09	Non-ionic
Brij® 58	0.007-0.077	Non-ionic
C₁₂E₈	0.11	Non-ionic
CHAPS	6-10	Zwitterionic
CHAPSO	8	Zwitterionic
CTAB	1	Cationic
Cyclohexyl-n-hexyl-β-D-maltoside	0.56	Non-ionic
DDMAB	4.3	Zwitterionic
Deoxy Big CHAP	1.1-1.4	Non-ionic
Deoxycholate	4-8	Anionic
Digitonin	0.2-0.73	Non-ionic
HECAMEG	19.5	Non-ionic
MEGA 10	6-7	Non-ionic
MEGA 8	58	Non-ionic
MEGA 9	19-25	Non-ionic
n-Decanoylsucrose	2.5	Non-ionic
n-Decyl-β-D-maltopyranoside	1.6	Non-ionic
n-Dodecanoylsucrose	0.3	Non-ionic
n-Dodecyl-β-D-glucopyranoside	0.13	Non-ionic
n-Dodecyl-β-D-maltoside	0.1-0.6	Non-ionic
n-Heptyl-β-D-glucopyranoside	79	Non-ionic
n-Heptyl-β-D-thioglucopyranoside	30	Non-ionic
n-Hexyl-β-D-glucopyranoside	250	Non-ionic
n-Nonyl-β-D-glucopyranoside	6.5	Non-ionic
n-Octanoylsucrose	24.4	Non-ionic
n-Octyl-β-D-glucopyranoside	20-25	Non-ionic
n-Octyl-β-D-maltopyranoside	23.4	Non-ionic
n-Octyl-β-D-thioglucopyranoside	9	Non-ionic
Nonidet® P-40 Substitute	0.05-0.3	Non-ionic
Octyl β Glucoside	20-25	Non-ionic
SDS	7-10	Anionic
Sulfobetaine 3-10 (SB 3-10)	25-40	Zwitterionic
Sulfobetaine 3-12 (SB 3-12)	2-4	Zwitterionic
Sulfobetaine 3-14 (SB 3-14)	0.1-0.4	Zwitterionic
Triton® X-100	0.2	Non-ionic
Triton® X-114	0.35	Non-ionic
Tween® 20	0.06	Non-ionic
Tween® 80	0.012	Non-ionic
ZWITTERGENT® 3-10	25-40	Zwitterionic
ZWITTERGENT® 3-12	2-4	Zwitterionic
ZWITTERGENT® 3-14	0.1-0.4	Zwitterionic
ZWITTERGENT® 3-16	0.01-0.06	Zwitterionic

References

This online resource may be cited as follows

MLA	"Quest Database™ Triton® X-100 Detergent." AAT Bioquest, Inc., 23 Sep. 2025, https://www.aatbio.com/resources/biological-detergents-properties-and-applications/triton-x-100.
APA	AAT Bioquest, Inc. (2025, September 23). Quest Database™ Triton® X-100 Detergent. AAT Bioquest. https://www.aatbio.com/resources/biological-detergents-properties-and-applications/triton-x-100.
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