CHAPS Detergent

Type:	Zwitterionic
Molecular Weight:	615
Molecular Formula:	C₃₂H₅₈N₂O₇S
Absorbance:
Critical Micelle Concentration (CMC)^(?):	6-10
Aggregation number	10
Average micellar weight	6,150
Cloud point	>100°C
Conductivity	<25µS in a 10% solution
Solubility	Water soluble
Applications:	CHAPS is a non-denaturing, electrically neutral, sulfobetaine dtergent. It is used for solubilizing membrane proteins and breaking protein-protein interactions. It causes less protein aggregation than non ionic detergents and is capable of solubilizing poiate receptors. CHAPS can be removed from proteins by detergent removing gel or dialysis.
Chemical Structure:

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Detergents ▲ ▼	CMC ▲ ▼
ASB-14	8
ASB-14-4	-
ASB-16	8
ASB-C7BzO	-
ASB-C8Ø	-
Big CHAP	3.4
Brij® 35	0.09
Brij® 58	0.007-0.077
C₁₂E₈	0.11
CHAPS	6-10
CHAPSO	8
CTAB	1
Cyclohexyl-n-hexyl-β-D-maltoside	0.56
DDMAB	4.3
Deoxy Big CHAP	1.1-1.4
Deoxycholate	4-8
Digitonin	0.2-0.73
HECAMEG	19.5
MEGA 10	6-7
MEGA 8	58
MEGA 9	19-25
n-Decanoylsucrose	2.5
n-Decyl-β-D-maltopyranoside	1.6
n-Dodecanoylsucrose	0.3
n-Dodecyl-β-D-glucopyranoside	0.13
n-Dodecyl-β-D-maltoside	0.1-0.6
n-Heptyl-β-D-glucopyranoside	79
n-Heptyl-β-D-thioglucopyranoside	30
n-Hexyl-β-D-glucopyranoside	250
n-Nonyl-β-D-glucopyranoside	6.5
n-Octanoylsucrose	24.4
n-Octyl-β-D-glucopyranoside	20-25
n-Octyl-β-D-maltopyranoside	23.4
n-Octyl-β-D-thioglucopyranoside	9
Nonidet® P-40 Substitute	0.05-0.3
Octyl β Glucoside	20-25
SDS	7-10
Sulfobetaine 3-10 (SB 3-10)	25-40
Sulfobetaine 3-12 (SB 3-12)	2-4
Sulfobetaine 3-14 (SB 3-14)	0.1-0.4
Triton® X-100	0.2
Triton® X-114	0.35
Tween® 20	0.06
Tween® 80	0.012
ZWITTERGENT® 3-10	25-40
ZWITTERGENT® 3-12	2-4
ZWITTERGENT® 3-14	0.1-0.4
ZWITTERGENT® 3-16	0.01-0.06

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