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AAT Bioquest

FAQ

Posted on July 16, 2024
How does chromatin immunoprecipitation sequencing (ChIP-Seq) work?
Antibodies and ProteomicsChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationGene Expression Analysis & Genotyping


Posted on July 16, 2024
What are the applications of chromatin immunoprecipitation sequencing (ChIP-Seq)?
Antibodies and ProteomicsChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationGene Expression Analysis & Genotyping


Posted on July 16, 2024
What is lysate immunoprecipitation?
Antibodies and ProteomicsChromatin Immunoprecipitation (ChIP)Purification


Posted on July 16, 2024
What is a ChIP assay?
Antibodies and ProteomicsChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationGene Expression Analysis & Genotyping


Posted on July 16, 2024
What kinds of immunoprecipitation procedures exist?
Antibodies and ProteomicsChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationGene Expression Analysis & GenotypingPurification


Posted on July 16, 2024
Is immunoprecipitation expensive?
Antibodies and ProteomicsChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationGene Expression Analysis & GenotypingPurification


Posted on July 16, 2024
Who invented immunoprecipitation?
Antibodies and ProteomicsChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationGene Expression Analysis & GenotypingPurification


Posted on July 16, 2024
How do I lower my background signal in my immunoprecipitation assay?
Antibodies and ProteomicsChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationGene Expression Analysis & GenotypingPurification


Posted on July 16, 2024
Can I use beads for my immunoprecipitation assay?
Antibodies and ProteomicsChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationGene Expression Analysis & GenotypingPurification


Posted on April 12, 2024
What should I consider in order to get the best chromatin immunoprecipitation (ChIP) results in my experiment?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
What are the common fixatives for ChIP experiments?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
How long should cells be fixed for my ChIP experiment?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
What are the different types of antibodies available for use in ChIP assays?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
Is there one type of antibody better than the others for use in ChIP assays?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
What should I do if there isn't a ChIP-validated antibody for my target protein?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
What are the benefits of using magnetic beads for immunoprecipitations?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
What are the benefits of using agarose beads for immunoprecipitations?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
What are the main criteria to consider when optimizing fixation conditions for ChIP experiments?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
What are the consequences of under-fixation or over-fixation?
BioconjugationAntibodies and ProteomicsAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
What is native ChIP?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
How do I isolate nuclei before my ChIP experiment?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleuscell lysis


Posted on April 12, 2024
Is nuclear isolation required for ChIP assays?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
How does sonication work?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
What are the disadvantages of using multiple-sample sonicators for chromatin fragmentation?
Cellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationDNA FragmentationNucleic AcidsNucleus


Posted on April 12, 2024
What factors should I consider when performing single-sample probe sonication for my ChIP experiment?
Cellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationDNA FragmentationNucleic AcidsNucleus


Posted on April 12, 2024
What are the important considerations when performing multi-sample sonication for my ChIp experiment?
Antibodies and ProteomicsAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
What are the alternatives to traditional ChIP assays?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
What should I do if I have barely enough cells to perform ChIP assays?
Cellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
How do I perform ChIP assays with FFPE tissue samples?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 12, 2024
What methods can be used to analyze chromatin immunoprecipitation (ChIP) results?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on July 28, 2023
What are the differences between chromatin and chromatids?
Cell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)


Posted on July 28, 2023
In what phase does chromatin coil and condense, forming chromosomes?
Cell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)


Posted on July 28, 2023
What are the functions of chromatin?
Cell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)


Posted on July 28, 2023
How is chromatin activity regulated?
Cell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)


Posted on July 28, 2023
What are some common techniques to analyze chromatin activity?
BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)Protein


Posted on July 28, 2023
Can chromatin activity be manipulated for therapeutic purposes?
BioconjugationCell Cycle AnalysisCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)Protein


Posted on July 28, 2023
How does histone modification affect chromatin structure?
Cell Cycle AnalysisCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)Protein


Posted on July 28, 2023
What are the disadvantages of native ChIP?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)Protein


Posted on July 28, 2023
What are the advantages of Cross-linking ChIP?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)Protein


Posted on July 28, 2023
How does ChIP work?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)Protein


Posted on July 28, 2023
What are the limitations of ChIP?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)Protein


Posted on July 28, 2023
What are the factors to consider when planning ChIP assays?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)Protein


Posted on July 28, 2023
What is chromatin made of?
Cell Cycle AnalysisCell Cycle AssaysCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)


Posted on July 28, 2023
Why is chromatin structure important?
Cellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)


Posted on July 28, 2023
Where is chromatin found?
Cell Cycle AnalysisCell Cycle AssaysCellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)


Posted on July 28, 2023
What are the types of chromatin?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)


Posted on July 28, 2023
Which type of chromatin is generally more active?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)