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Sample Preparations
AAT Bioquest offers a comprehensive range of sample preparation reagents and kits designed to streamline workflows before downstream analysis. From cell lysis and protein extraction to nucleic acid purification and virus concentration, these products ensure optimal sample quality for assays, sequencing, and imaging applications.
Our Sample Prep Technologies
ReadiPrep™ — Kits for sample cleanup, magnetic beads based purification, nucleic acid extraction
ReadiUse™ — Reagents including lysis buffers, spin columns
ReadiUse™ — Reagents including lysis buffers, spin columns
Browse Sample Preparations
Cell Culture and Lysis
Cell culture and lysis reagents support cell growth, harvesting, fixation, and lysis for downstream applications.
- ReadiUse™ cell lysis buffers optimized for mammalian, bacterial, yeast, and viral samples
- ReadiUse™ Cell Detaching Buffer for gentle, animal-free cell harvesting
- ReadiUse™ 4% formaldehyde fixation solution and RBC lysis buffers for blood processing
Mounting Media
Mounting media preserve fluorescent signals in microscopy samples.
- Antifade formulations to reduce photobleaching
- Hardening and non-hardening options
- Compatible with multiple fluorophores
Cell Transfection
Transfection reagents enable delivery of DNA, RNA, and proteins into mammalian cells.
- Lipid-based transfection reagents
- Optimized for various cell types
- Low cytotoxicity formulations
- Compatible with plasmid DNA, siRNA, and mRNA
Buffers
Buffer solutions provide optimized environments for assays, reactions, and sample storage.
- General laboratory buffers including PBS tablets, HHBS, and citrate antigen retrieval buffer
- ReadiUse™ pre-formulated buffers for conjugation, PCR, TSA/PSA signal amplification, and kinase assays
- Dye loading enhancers (Pluronic® F-127 and Probenecid) for AM ester calcium indicator assays
- CytoWatch™ and Screen Quest™ buffers for flow cytometry multiplexing and high-throughput screening
Magnetic Beads and Kits
Magnetic bead reagents enable rapid purification without centrifugation.
- Protein A/G beads for antibody purification
- Streptavidin beads for biotin-tagged molecules
- Silica beads for nucleic acid isolation
- Functionalized beads for custom applications
Protein Extraction and Purification
Protein extraction and purification reagents isolate and purify proteins from cells, tissues, and recombinant expression systems.
- ReadiPrep™ kits for histone, plasma membrane, and tissue protein extraction
- Ni-NTA magnetic beads and agarose resins for His-tag protein purification
- Protein A and Protein G agarose resins for antibody purification
- Sample cleanup tools including PD-10 desalting columns, spin filters, and TCA deproteinization kits
DNA Extraction and Purification
DNA extraction kits isolate genomic DNA from various sample types.
- Column-based and magnetic bead formats
- Optimized for cells, tissues, and blood
- High yield and purity for PCR and sequencing
- Scalable protocols for different sample amounts
RNA Extraction and Purification
RNA extraction kits isolate total RNA with minimal degradation.
- DNase treatment options included
- Column-based purification
- Compatible with RT-PCR and RNA-seq
- RNase-free reagents throughout
Cell Structure Isolation and Fractionation
Organelle isolation kits enable purification of subcellular compartments.
- Nuclear isolation for chromatin studies
- Mitochondria purification for metabolism research
- Membrane fractionation protocols
- Validated for multiple cell types
Resins and Columns
Chromatography resins and columns support purification and buffer exchange.
- Size exclusion resins for desalting
- Ion exchange and affinity resins
- Pre-packed spin columns for convenience
- Bulk resins for preparative separations
This document (01.0334.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.