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5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9*

Chemical structure for 5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9*
Chemical structure for 5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9*
Ordering information
Price ()
Catalog Number100
Unit Size
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Physical properties
Molecular weight376.32
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)83000
Excitation (nm)493
Emission (nm)517
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
72088-94-9
Molecular weight
376.32
Correction Factor (260 nm)
0.32
Correction Factor (280 nm)
0.178
Extinction coefficient (cm -1 M -1)
83000
Excitation (nm)
493
Emission (nm)
517
Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. FITC-labeled nucleotides and peptides tend to deteriorate more quickly than the corresponding FAM conjugates. We found that FAM reagents can be used to substitute FITC reagents in most biological applications.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM265.731 µL1.329 mL2.657 mL13.287 mL26.573 mL
5 mM53.146 µL265.731 µL531.463 µL2.657 mL5.315 mL
10 mM26.573 µL132.866 µL265.731 µL1.329 mL2.657 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)83000
Excitation (nm)493
Emission (nm)517

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7*493517830000.320.178
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8*493517830000.320.178
5-FAM ethylenediamine493517830000.320.178
5-FAM cadaverine493517830000.320.178
5-FAM Azide493517830000.320.178
5-FAM Alkyne493517830000.320.178
6-FAM Azide493517830000.320.178
6-FAM Alkyne493517830000.320.178
6-FAM phosphoramidite [5'-Fluorescein phosphoramidite] *CAS 204697-37-0*493517830000.320.178
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]522546940000.240.214
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6*552578900000.320.178
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*57860482000-0.168
5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8*5445701000000.270.34
Show More (14)

Citations


View all 5 citations: Citation Explorer
Intracellular delivery of a peptide nucleic acid-based hybrid of an autophagy inducing peptide with a cell-penetrating peptide
Authors: Hakata, Yoshiyuki and Ishikawa, Suzuka and Ohtsuki, Takashi and Miyazawa, Masaaki and Kitamatsu, Mizuki
Journal: Organic \& Biomolecular Chemistry (2020): 1978--1986
Acidic pH-Activated Gas-Generating Nanoparticles with Pullulan Decorating for Hepatoma-Targeted Ultrasound Imaging
Authors: Chen, Shanshan and Xu, Xiao-Lin and Zhou, Boyang and Tian, Jing and Luo, Baoming and Zhang, Li-Ming
Journal: ACS Applied Materials & Interfaces (2019)
Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Journal: Advanced Materials (2017)
Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
Journal: Scientific reports (2015)
Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

References


View all 137 references: Citation Explorer
Carboxyfluorescein leakage from poly(ethylene glycol)-grafted liposomes induced by the interaction with serum
Authors: Hashizaki K, Taguchi H, Sakai H, Abe M, Saito Y, Ogawa N.
Journal: Chem Pharm Bull (Tokyo) (2006): 80
Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (cfse)
Authors: Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L.
Journal: Proc Biol Sci (2006): 1165
Application of alamar blue/5-carboxyfluorescein diacetate acetoxymethyl ester as a noninvasive cell viability assay in primary hepatocytes from rainbow trout
Authors: Schreer A, Tinson C, Sherry JP, Schirmer K.
Journal: Anal Biochem (2005): 76
Low incidence of acute rejection after living-donor liver transplantation: Immunologic analyses by mixed lymphocyte reaction using a carboxyfluorescein diacetate succinimidyl ester labeling technique
Authors: Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, Asahara T.
Journal: Transplantation (2005): 1262
Evidence for dimer formation by an amphiphilic heptapeptide that mediates chloride and carboxyfluorescein release from liposomes
Authors: Pajewski R, Ferdani R, Pajewska J, Djedovic N, Schlesinger PH, Gokel GW.
Journal: Org Biomol Chem (2005): 619
Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling
Authors: Wang XQ, Duan XM, Liu LH, Fang YQ, Tan Y.
Journal: Acta Biochim Biophys Sin (Shanghai) (2005): 379
Application of glutaraldehyde for the staining of esterase-active cells with carboxyfluorescein diacetate
Authors: Morono Y, Takano S, Miyanaga K, Tanji Y, Unno H, Hori K.
Journal: Biotechnol Lett (2004): 379
Drug efflux transport properties of 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (bcecf-am) and its fluorescent free acid, bcecf
Authors: Bachmeier CJ, Trickler WJ, Miller DW.
Journal: J Pharm Sci (2004): 932
pruning of alloreactive cd4+ t cells using 5- (and 6-)carboxyfluorescein diacetate succinimidyl ester prolongs skin allograft survival
Authors: Watson D, Zhang GY, Sartor M, Alex and er SI., undefined
Journal: J Immunol (2004): 6574
Cyclodextrin enhanced transdermal delivery of piroxicam and carboxyfluorescein by electroporation
Authors: Murthy SN, Zhao YL, Sen A, Hui SW.
Journal: J Control Release (2004): 393