AAT Bioquest

5-dROX [5-Carboxy-4,7-dichloror-X-hodamine]

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Physical properties
Molecular weight704.69
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
5-dROX (5-Carboxy-4,7-dichloror-X-hodamine) is one of the four critical 4,7-dichloro-rhodamine dyes used as the BigDye terminators for DNA sequencing in combination with dR110, dR6G and dTMR. ABI PRISM® BigDye™ Terminator Cycle Sequencing is the classic method for DNA sequencing.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-dROX [5-Carboxy-4,7-dichloror-X-hodamine] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM141.906 µL709.532 µL1.419 mL7.095 mL14.191 mL
5 mM28.381 µL141.906 µL283.813 µL1.419 mL2.838 mL
10 mM14.191 µL70.953 µL141.906 µL709.532 µL1.419 mL

Molarity calculator

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View all 6 references: Citation Explorer
Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing
Authors: Tan, L.; Liu, Y.; Yang, Q.; Li, X.; Wu, X. Y.; Gong, B.; Shen, Y. M.; Shao, Z.
Journal: Chem Commun (Camb) (2016): 954-7
Synthesis of novel tyrosinyl FRET cassettes, terminators, and their potential use in DNA sequencing
Authors: Sudhakar Rao, T.; Zhang, W.; Xiao, H.; Flick, P.; Kumar, S.; Nampalli, S.
Journal: Nucleosides Nucleotides Nucleic Acids (2003): 1443-5
Design strategies and performance of custom DNA sequencing primers
Authors: Buck, G. A.; Fox, J. W.; Gunthorpe, M.; Hager, K. M.; Naeve, C. W.; Pon, R. T.; Adams, P. S.; Rush, J.
Journal: Biotechniques (1999): 528-36
Improved DNA sequencing accuracy and detection of heterozygous alleles using manganese citrate and different fluorescent dye terminators
Authors: Korch, C.; Drabkin, H.
Journal: Genome Res (1999): 588-95
Peak height pattern in dichloro-rhodamine and energy transfer dye terminator sequencing
Authors: Zakeri, H.; Amparo, G.; Chen, S. M.; Spurgeon, S.; Kwok, P. Y.
Journal: Biotechniques (1998): 406-10, 412-4
New dye-labeled terminators for improved DNA sequencing patterns
Authors: Rosenblum, B. B.; Lee, L. G.; Spurgeon, S. L.; Khan, S. H.; Menchen, S. M.; Heiner, C. R.; Chen, S. M.
Journal: Nucleic Acids Res (1997): 4500-4