5-dROX [5-Carboxy-4,7-dichloror-X-hodamine]

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Physical properties

Molecular weight	704.69
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

704.69

5-dROX (5-Carboxy-4,7-dichloror-X-hodamine) is one of the four critical 4,7-dichloro-rhodamine dyes used as the BigDye terminators for DNA sequencing in combination with dR110, dR6G and dTMR. ABI PRISM® BigDye™ Terminator Cycle Sequencing is the classic method for DNA sequencing.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-dROX [5-Carboxy-4,7-dichloror-X-hodamine] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	141.906 µL	709.532 µL	1.419 mL	7.095 mL	14.191 mL
5 mM	28.381 µL	141.906 µL	283.813 µL	1.419 mL	2.838 mL
10 mM	14.191 µL	70.953 µL	141.906 µL	709.532 µL	1.419 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
5-FAM [5-Carboxyfluorescein] CAS 76823-03-5	493	517	83000	0.178
5-SFX [5-FAM-X, SE] CAS 148356-00-7	493	517	83000	0.178
5-FITC [Fluorescein-5-isothiocyanate] CAS 3326-32-7	491	516	73000	0.35
5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] CAS 51306-35-5	498	517	80000¹	0.35
5-IAF [5-Iodoacetamidofluorescein] CAS 63368-54-7	498	517	80000¹	0.35
5-CR110 [5-Carboxyrhodamine 110] Single isomer	499	525	84000	0.091
5-CR6G [5-Carboxyrhodamine 6G] Single isomer	522	546	94000	0.214
5-TAMRA [5-Carboxytetramethylrhodamine] CAS 91809-66-4	552	578	90000	0.178
5-ROX [5-Carboxy-X-rhodamine] CAS 216699-35-3	578	604	82000	0.168
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] CAS 80724-19-2	544	570	100000	0.34
DiBAC4(5) [Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol]	591	615	-	-
DiSBAC2(5) [Bis-(1,3-diethylthiobarbituric acid)pentamethine oxonol]	636	655	-	-
Show More (3)

Images

Figure 1. Chemical structure for 5-dROX [5-Carboxy-4,7-dichloror-X-hodamine]

References

View all 6 references: Citation Explorer

Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing
Authors: Tan, L.; Liu, Y.; Yang, Q.; Li, X.; Wu, X. Y.; Gong, B.; Shen, Y. M.; Shao, Z.
Journal: Chem Commun (Camb) (2016): 954-7

Synthesis of novel tyrosinyl FRET cassettes, terminators, and their potential use in DNA sequencing
Authors: Sudhakar Rao, T.; Zhang, W.; Xiao, H.; Flick, P.; Kumar, S.; Nampalli, S.
Journal: Nucleosides Nucleotides Nucleic Acids (2003): 1443-5

Improved DNA sequencing accuracy and detection of heterozygous alleles using manganese citrate and different fluorescent dye terminators
Authors: Korch, C.; Drabkin, H.
Journal: Genome Res (1999): 588-95

Design strategies and performance of custom DNA sequencing primers
Authors: Buck, G. A.; Fox, J. W.; Gunthorpe, M.; Hager, K. M.; Naeve, C. W.; Pon, R. T.; Adams, P. S.; Rush, J.
Journal: Biotechniques (1999): 528-36

Peak height pattern in dichloro-rhodamine and energy transfer dye terminator sequencing
Authors: Zakeri, H.; Amparo, G.; Chen, S. M.; Spurgeon, S.; Kwok, P. Y.
Journal: Biotechniques (1998): 406-10, 412-4

New dye-labeled terminators for improved DNA sequencing patterns
Authors: Rosenblum, B. B.; Lee, L. G.; Spurgeon, S. L.; Khan, S. H.; Menchen, S. M.; Heiner, C. R.; Chen, S. M.
Journal: Nucleic Acids Res (1997): 4500-4