Name: Amplite® Fluorimetric NADPH Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 15262
Price: 355 USD
Availability: InStock

Amplite® Fluorimetric NADPH Assay Kit

Red Fluorescence

Amplite® Fluorimetric NADPH Assay Kit *Red Fluorescence* is a fluorimetric assay kit for quantification of NADPH in a microplate format.

Detection Principle: Uses an enzyme-cycling reaction that specifically recognizes NADPH.
Sensitivity: Detects NADPH down to 1 µM.
Workflow: Continuous assay with no separation step; compatible with 96- or 384-well plates and automation.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm; absorbance can also be measured at 575 nm.

NADPH dose response was measured with Amplite® Fluorimetric NADPH Assay Kit in a 96-well solid black plate using a NOVOStar microplate reader (BMG Labtech). RFU measured over Ex/Em = 540/590 nm.

Protocol

SDS

Catalog	Size	Price	Quantity
15262	400 Tests	Price

Overview

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+, and NAD+ is the oxidized form of NADH. It forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. NADP is used in anabolic biological reactions, such as fatty acid and nucleic acid synthesis, which require NADPH as a reducing agent. In chloroplasts, NADP is an oxidizing agent important in the preliminary reactions of photosynthesis. The NADPH produced by photosynthesis is then used as reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis. The traditional NAD/NADH and NADP/NADPH assays are done by monitoring of NADH or NADPH absorption at 340 nm. This method suffers low sensitivity and high interference since the assay is done in the UV range that requires expensive quartz microplate. This Amplite® NADPH Assay Kit provides a convenient method for sensitive detection of NADPH. The enzymes in the system specifically recognize NADPH in an enzyme cycling reaction. The enzyme cycling reaction significantly increases detection sensitivity.

Citations

View all 66 citations: Citation Explorer

Peroxiredoxin-1 is an H2O2 safe-guard antioxidant and signalling enzyme in M1 macrophages

Authors:

Ezeri{\c{n}}a, Daria and Vo, Trung Nghia and Luo, Ting and Elkrim, Yvon and Suarez, Anna Escoda and Herinckx, Ga{\"e}tan and Vertommen, Didier and Laoui, Damya and Van Ginderachter, Jo A and Messens, Joris

Journal:

Advances in Redox Research (2023): 100083

The Linkage of Yeast Metabolites, Produced Under Hyperosmotic Stress, to Cellular Cofactor Systems During Icewine Fermentation.

Authors:

Allie, Robert

A mechanistic insight into the bioaccesible herbometallic nanodrug as potential dual therapeutic agent

Authors:

Bera, Debbethi and Pal, Kunal and Ruidas, Bhuban and Mondal, Dheeraj and Pal, Shinjini and Paul, Biplab Kumar and Karmakar, Parimal and Das, Sukhen and Nandy, Papiya

Journal:

Materials Today Communications (2020): 101099

Guar gum micro-vehicle mediated delivery strategy and synergistic activity of thymoquinone and piperine: An in vitro study on bacterial and hepatocellular carcinoma cells

Authors:

Das, Sanghita and Bera, Debbethi and Pal, Kunal and Mondal, Dheeraj and Karmakar, Parimal and Das, Sukhen and Dey, Anindita

Journal:

Journal of Drug Delivery Science and Technology (2020): 101994

Safflor Yellow B Attenuates Ischemic Brain Injury via Downregulation of Long Noncoding AK046177 and Inhibition of MicroRNA-134 Expression in Rats

Authors:

Wang, Chaoyun and Wan, Hongzhi and Wang, Qiaoyun and Sun, Hongliu and Sun, Yeying and Wang, Kexin and Zhang, Chunxiang

Journal:

Oxidative Medicine and Cellular Longevity (2020)

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: NADPH Recycling Enzyme Mix	2 bottles (lyophilized powder)
Component B: NADPH Assay Buffer	1 bottle (20 mL)
Component C: NADPH Standard	1 vial (167 µg)