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Amplite® Fluorimetric NADPH Assay Kit
Red Fluorescence
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+, and NAD+ is the oxidized form of NADH. It forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. NADP is used in anabolic biological reactions, such as fatty acid and nucleic acid synthesis, which require NADPH as a reducing agent. In chloroplasts, NADP is an oxidizing agent important in the preliminary reactions of photosynthesis. The NADPH produced by photosynthesis is then used as reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis. The traditional NAD/NADH and NADP/NADPH assays are done by monitoring of NADH or NADPH absorption at 340 nm. This method suffers low sensitivity and high interference since the assay is done in the UV range that requires expensive quartz microplate. This Amplite® NADPH Assay Kit provides a convenient method for sensitive detection of NADPH. The enzymes in the system specifically recognize NADPH in an enzyme cycling reaction. The enzyme cycling reaction significantly increases detection sensitivity.
NADPH dose response was measured with Amplite® Fluorimetric NADPH Assay Kit in a 96-well solid black plate using a NOVOStar microplate reader (BMG Labtech). RFU measured over Ex/Em = 540/590 nm.
NADPH dose response was measured with Amplite® Fluorimetric NADPH Assay Kit in a 96-well solid black plate using a NOVOStar microplate reader (BMG Labtech). RFU measured over Ex/Em = 540/590 nm.
CatalogSize
Price
Quantity
15262400 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Recommended plateSolid black
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Page updated on September 25, 2025