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Calcium Indicators
AAT Bioquest offers the largest selection of calcium indicators, with options for different modalities, binding affinities, and emission colors to optimally fit your experimental needs.
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Binding Affinities
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Binding Affinity (Kd)
Emission Color
No Probenecid Required
Excitation Wavelength
200 nm
900 nm
Kits
No Wash
Emission Wavelength
200 nm
900 nm
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Recommended
Cal-520®-Dextran Conjugate MW 10,000
Catalog Number: 20601
Modality
Single-wavelength
Excitation/Emission (nm)
492/515
Kd (nM)
-
Cal-520®-Dextran Conjugate MW 3,000
Catalog Number: 20600
Modality
Single-wavelength
Excitation/Emission (nm)
492/515
Kd (nM)
-
Cal-520L®-Dextran Conjugate MW 10,000
Catalog Number: 20602
Modality
Single-wavelength
Excitation/Emission (nm)
-
Kd (nM)
-
Cal-590L® Dextran Conjugate MW 10,000
Catalog Number: 20525
Modality
Single-wavelength
Excitation/Emission (nm)
569/590
Kd (nM)
-
Cal-590™-Dextran Conjugate MW 10,000
Catalog Number: 20509
Modality
Single-wavelength
Excitation/Emission (nm)
563/584
Kd (nM)
-
Cal-590™-Dextran Conjugate MW 3,000
Catalog Number: 20508
Modality
Single-wavelength
Excitation/Emission (nm)
563/584
Kd (nM)
-
Cal-630™-Dextran Conjugate MW 10,000
Catalog Number: 20546
Modality
Single-wavelength
Excitation/Emission (nm)
609/626
Kd (nM)
-
Cal-670™-Dextran Conjugate MW 10,000
Catalog Number: 20457
Modality
Single-wavelength
Excitation/Emission (nm)
667/680
Kd (nM)
-
Cal-670™-Dextran Conjugate MW 3,000
Catalog Number: 20456
Modality
Single-wavelength
Excitation/Emission (nm)
667/680
Kd (nM)
-
Learn More
AAT Bioquest offers the largest portfolio of calcium indicators, with over one hundred unique reagents, cited in tens of thousands of publications. The table below offers a few example applications. To learn more about how to select the optimal indicator for your experiment, please see our Calcium Indicators catalog.
| What you're studying | What you're measuring | AAT Bioquest products to consider |
|---|---|---|
| Neuronal activity | Spontaneous activity, stimulus responses, and neuronal calcium transients | Cal-520® AM; Cal-590™ AM when combining calcium imaging with GFP. |
| Cardiac calcium handling | Beat-associated transients and changes in calcium release and recovery | Cal-520® AM or Calbryte™ 520 AM; evaluate signal kinetics and saturation in your model. |
| Immune-cell activation | Calcium flux after receptor stimulation in defined cell populations | Calbryte™ 520 AM for green-channel flow cytometry; Calbryte™ 630 AM for a compatible red-laser configuration. |
| Drug screening and receptor/channel assays | Compound-induced calcium responses and concentration–response curves | Screen Quest™ Calbryte™ 520 or Screen Quest™ Fluo-8® assays for streamlined, no-wash screening. |
| Calcium-store release and entry | Cytosolic calcium changes following store depletion or extracellular calcium addition | Fura-8™ AM for ratiometric measurements; Calbryte™ 520 AM for relative fluorescence changes. |
Resources & Tools
Calcium Indicators
Learn about the different factors when selecting an optimal calcium indicator
View Catalog
Cal-520® and Other Cal™ Indicators
Explore our Cal-520® and Other Cal™ Indicators digital catalog
View Catalog