Name: Cal-520L®-Dextran Conjugate *MW 10,000*
Brand: AAT Bioquest
SKU: 20602
Price: 348 USD
Availability: InStock

Cal-520L®-Dextran Conjugate

MW 10,000

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. This Cal-520L dextran exhibits low affinity to calcium ion than Cal-520 dextran, and optimized for monitoring high level calcium ion.

Ca2+ Dependent Dose Response of Cal-520L®-Dextran Conjugate

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20602	1 mg	Price

Citations

View all 52 citations: Citation Explorer

Molecular dynamics simulations reveal the influence of dextran sulfate in nanoparticle formation with calcium alginate to encapsulate insulin

Authors:

Nadvorny, D., Soares-Sobrinho, J. L., de La Roca Soares, M. F., Ribeiro, A. J., Veiga, F., Seabra, G. M.

Journal:

J Biomol Struct Dyn (2018): 1255-1260

Sustained Release of Minocycline From Minocycline-Calcium-Dextran Sulfate Complex Microparticles for Periodontitis Treatment

Authors:

Wu, L., Chen, W., Li, F., Morrow, B. R., Garcia-Godoy, F., Hong, L.

Journal:

J Pharm Sci (2018): 3134-3142

Calcium/Ask1/MKK7/JNK2/c-Src signalling cascade mediates disruption of intestinal epithelial tight junctions by dextran sulfate sodium

Authors:

Samak, G., Chaudhry, K. K., Gangwar, R., Narayanan, D., Jaggar, J. H., Rao, R.

Journal:

Biochem J (2015): 503-15

Monitoring of single-cell responses in the optic tectum of adult zebrafish with dextran-coupled calcium dyes delivered via local electroporation

Authors:

Kassing, V., Engelmann, J., Kurtz, R.

Journal:

PLoS One (2013): e62846

Forward-filling of dextran-conjugated indicators for calcium imaging at the Drosophila larval neuromuscular junction

Authors:

Macleod, G. T.

Journal:

Cold Spring Harb Protoc (2012): 791-6

Physical properties

Molecular weight	~11000
Solvent	Water

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	498 nm
Emission	521 nm
Recommended plate	Black wall/clear bottom

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Page updated on October 13, 2025