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Cal-520L®-Dextran Conjugate *MW 10,000*

OverviewpdfSDSpdfProtocol


Molecular weight
11000
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. This Cal-520L dextran exhibits low affinity to calcium ion than Cal-520 dextran, and optimized for monitoring high level calcium ion.

Platform


Fluorescence microscope

Excitation498 nm
Emission521 nm
Recommended plateBlack wall/clear bottom

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal-520L®-Dextran Conjugate *MW 10,000* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM9.091 µL45.455 µL90.909 µL454.545 µL909.091 µL
5 mM1.818 µL9.091 µL18.182 µL90.909 µL181.818 µL
10 mM909.091 nL4.545 µL9.091 µL45.455 µL90.909 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Citations


View all 52 citations: Citation Explorer
Molecular dynamics simulations reveal the influence of dextran sulfate in nanoparticle formation with calcium alginate to encapsulate insulin
Authors: Nadvorny, D., Soares-Sobrinho, J. L., de La Roca Soares, M. F., Ribeiro, A. J., Veiga, F., Seabra, G. M.
Journal: J Biomol Struct Dyn (2018): 1255-1260
Sustained Release of Minocycline From Minocycline-Calcium-Dextran Sulfate Complex Microparticles for Periodontitis Treatment
Authors: Wu, L., Chen, W., Li, F., Morrow, B. R., Garcia-Godoy, F., Hong, L.
Journal: J Pharm Sci (2018): 3134-3142
Calcium/Ask1/MKK7/JNK2/c-Src signalling cascade mediates disruption of intestinal epithelial tight junctions by dextran sulfate sodium
Authors: Samak, G., Chaudhry, K. K., Gangwar, R., Narayanan, D., Jaggar, J. H., Rao, R.
Journal: Biochem J (2015): 503-15
Monitoring of single-cell responses in the optic tectum of adult zebrafish with dextran-coupled calcium dyes delivered via local electroporation
Authors: Kassing, V., Engelmann, J., Kurtz, R.
Journal: PLoS One (2013): e62846
Forward-filling of dextran-conjugated indicators for calcium imaging at the Drosophila larval neuromuscular junction
Authors: Macleod, G. T.
Journal: Cold Spring Harb Protoc (2012): 791-6
Presynaptic imaging of projection fibers by in vivo injection of dextran-conjugated calcium indicators
Authors: Brenowitz, S. D., Regehr, W. G.
Journal: Cold Spring Harb Protoc (2012): 465-71
Calcium alginate/dextran methacrylate IPN beads as protecting carriers for protein delivery
Authors: D'Arrigo, G., Di Meo, C., Pescosolido, L., Coviello, T., Alhaique, F., Matricardi, P.
Journal: J Mater Sci Mater Med (2012): 1715-22
Calcium ion-dependent increase in thermostability of dextran glucosidase from Streptococcus mutans
Authors: Kobayashi, M., Hondoh, H., Mori, H., Saburi, W., Okuyama, M., Kimura, A.
Journal: Biosci Biotechnol Biochem (2011): 1557-63
In situ forming IPN hydrogels of calcium alginate and dextran-HEMA for biomedical applications
Authors: Pescosolido, L., Vermonden, T., Malda, J., Censi, R., Dhert, W. J., Alhaique, F., Hennink, W. E., Matricardi, P.
Journal: Acta Biomater (2011): 1627-33
Loading neurons with dextran-conjugated calcium indicators in intact nervous tissue
Authors: Delaney, K. R.
Journal: Curr Protoc Neurosci (2010): Unit2 5