Cal-520L®-Dextran Conjugate MW 10,000

Ca2+ Dependent Dose Response of Cal-520L®-Dextran Conjugate

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Physical properties

Molecular weight	~11000
Solvent	Water

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

~11000

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. This Cal-520L dextran exhibits low affinity to calcium ion than Cal-520 dextran, and optimized for monitoring high level calcium ion.

Platform

Fluorescence microscope

Excitation	498 nm
Emission	521 nm
Recommended plate	Black wall/clear bottom

Product Family

Name	Excitation (nm)	Emission (nm)	Quantum yield
Cal-520®-Dextran Conjugate MW 3,000	493	515	0.75¹
Cal-520®-Dextran Conjugate MW 10,000	493	515	0.75¹
Cal-590™-Dextran Conjugate MW 3,000	563	584	0.62¹
Cal-590™-Dextran Conjugate MW 10,000	563	584	0.62¹
Cal-630™-Dextran Conjugate MW 3,000	609	626	0.37¹
Cal-630™-Dextran Conjugate MW 10,000	609	626	0.37¹
Cal-670™-Dextran Conjugate MW 3,000	667	680	-
Cal-670™-Dextran Conjugate MW 10,000	667	680	-
Cal-770™-Dextran Conjugate MW 3,000	758	783	-
Cal-770™-Dextran Conjugate MW 10,000	758	783	-
Cal-590L® Dextran Conjugate MW 10,000	569	590	-
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Images

Figure 1. Ca2+ Dependent Dose Response of Cal-520L®-Dextran Conjugate

Citations

View all 52 citations: Citation Explorer

Molecular dynamics simulations reveal the influence of dextran sulfate in nanoparticle formation with calcium alginate to encapsulate insulin
Authors: Nadvorny, D., Soares-Sobrinho, J. L., de La Roca Soares, M. F., Ribeiro, A. J., Veiga, F., Seabra, G. M.
Journal: J Biomol Struct Dyn (2018): 1255-1260

Sustained Release of Minocycline From Minocycline-Calcium-Dextran Sulfate Complex Microparticles for Periodontitis Treatment
Authors: Wu, L., Chen, W., Li, F., Morrow, B. R., Garcia-Godoy, F., Hong, L.
Journal: J Pharm Sci (2018): 3134-3142

Calcium/Ask1/MKK7/JNK2/c-Src signalling cascade mediates disruption of intestinal epithelial tight junctions by dextran sulfate sodium
Authors: Samak, G., Chaudhry, K. K., Gangwar, R., Narayanan, D., Jaggar, J. H., Rao, R.
Journal: Biochem J (2015): 503-15

Monitoring of single-cell responses in the optic tectum of adult zebrafish with dextran-coupled calcium dyes delivered via local electroporation
Authors: Kassing, V., Engelmann, J., Kurtz, R.
Journal: PLoS One (2013): e62846

Forward-filling of dextran-conjugated indicators for calcium imaging at the Drosophila larval neuromuscular junction
Authors: Macleod, G. T.
Journal: Cold Spring Harb Protoc (2012): 791-6

Presynaptic imaging of projection fibers by in vivo injection of dextran-conjugated calcium indicators
Authors: Brenowitz, S. D., Regehr, W. G.
Journal: Cold Spring Harb Protoc (2012): 465-71

Calcium alginate/dextran methacrylate IPN beads as protecting carriers for protein delivery
Authors: D'Arrigo, G., Di Meo, C., Pescosolido, L., Coviello, T., Alhaique, F., Matricardi, P.
Journal: J Mater Sci Mater Med (2012): 1715-22

Calcium ion-dependent increase in thermostability of dextran glucosidase from Streptococcus mutans
Authors: Kobayashi, M., Hondoh, H., Mori, H., Saburi, W., Okuyama, M., Kimura, A.
Journal: Biosci Biotechnol Biochem (2011): 1557-63

In situ forming IPN hydrogels of calcium alginate and dextran-HEMA for biomedical applications
Authors: Pescosolido, L., Vermonden, T., Malda, J., Censi, R., Dhert, W. J., Alhaique, F., Hennink, W. E., Matricardi, P.
Journal: Acta Biomater (2011): 1627-33

Loading neurons with dextran-conjugated calcium indicators in intact nervous tissue
Authors: Delaney, K. R.
Journal: Curr Protoc Neurosci (2010): Unit2 5