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Calcium Indicators
AAT Bioquest offers the largest selection of calcium indicators, with options for different modalities, binding affinities, and emission colors to optimally fit your experimental needs.
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Binding Affinities
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Binding Affinity (Kd)
Emission Color
No Probenecid Required
Excitation Wavelength
200 nm
900 nm
Kits
No Wash
Emission Wavelength
200 nm
900 nm
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Recommended
Cal-520®, AM
Catalog Number: 21130, 21131
Modality
Single-wavelength
Excitation/Emission (nm)
492/515
Kd (nM)
320
Fluo-8®, AM
Catalog Number: 21080, 21081, 21082, 21083
Modality
Single-wavelength
Excitation/Emission (nm)
495/516
Kd (nM)
389
Calbryte™ 520 AM
Catalog Number: 20650, 20651, 20653
Modality
Single-wavelength
Excitation/Emission (nm)
493/515
Kd (nM)
1200
Screen Quest™ Fluo-8® No Wash Calcium Assay Kit
Catalog Number: 36314, 36315, 36316
Modality
Single-wavelength
Excitation/Emission (nm)
495/516
Kd (nM)
-
Cal Green™ 1, AM [Equivalent to Calcium Green-1, AM]
Catalog Number: 20501, 20502
Modality
Single-wavelength
Excitation/Emission (nm)
498/517
Kd (nM)
190
Cal Green™ 1, hexapotassium salt
Catalog Number: 20500
Modality
Single-wavelength
Excitation/Emission (nm)
498/517
Kd (nM)
190
Cal-520® alkyne
Catalog Number: 20614
Modality
Single-wavelength
Excitation/Emission (nm)
492/515
Kd (nM)
-
Cal-520® amine
Catalog Number: 20612
Modality
Single-wavelength
Excitation/Emission (nm)
492/515
Kd (nM)
-
Cal-520® azide
Catalog Number: 20613
Modality
Single-wavelength
Excitation/Emission (nm)
492/515
Kd (nM)
-
Learn More
AAT Bioquest offers the largest portfolio of calcium indicators, with over one hundred unique reagents, cited in tens of thousands of publications. The table below offers a few example applications. To learn more about how to select the optimal indicator for your experiment, please see our Calcium Indicators catalog.
| What you're studying | What you're measuring | AAT Bioquest products to consider |
|---|---|---|
| Neuronal activity | Spontaneous activity, stimulus responses, and neuronal calcium transients | Cal-520® AM; Cal-590™ AM when combining calcium imaging with GFP. |
| Cardiac calcium handling | Beat-associated transients and changes in calcium release and recovery | Cal-520® AM or Calbryte™ 520 AM; evaluate signal kinetics and saturation in your model. |
| Immune-cell activation | Calcium flux after receptor stimulation in defined cell populations | Calbryte™ 520 AM for green-channel flow cytometry; Calbryte™ 630 AM for a compatible red-laser configuration. |
| Drug screening and receptor/channel assays | Compound-induced calcium responses and concentration–response curves | Screen Quest™ Calbryte™ 520 or Screen Quest™ Fluo-8® assays for streamlined, no-wash screening. |
| Calcium-store release and entry | Cytosolic calcium changes following store depletion or extracellular calcium addition | Fura-8™ AM for ratiometric measurements; Calbryte™ 520 AM for relative fluorescence changes. |
Resources & Tools
Calcium Indicators
Learn about the different factors when selecting an optimal calcium indicator
View Catalog
Cal-520® and Other Cal™ Indicators
Explore our Cal-520® and Other Cal™ Indicators digital catalog
View Catalog