Tide Quencher™ 7.2WS maleimide [TQ7.2WS maleimide]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1100.16 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 7.2WS amine [TQ7.2WS amine] |
Tide Quencher™ 7.2WS acid [TQ7.2WS acid] |
Tide Quencher™ 7.2WS azide [TQ7.2WS azide] |
Tide Quencher™ 7.2WS alkyne [TQ7.2WS alkyne] |
Related products
Overview | SDSProtocol |
See also: Tide Quencher Dyes
Molecular weight 1100.16 |
Tide Quencher™ 7.2WS (TQ7.2WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy7, Alexa Fluor® 700, Alexa Fluor® 750, iFluor® 700, iFluor® 710, iFluor® 720 and iFluor® 750. It is an improved version of TQ7 and BHQ3. TQ7.2WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for NIR dyes. Tide Quencher™ 7.2WS maleimide is primarily used for the post-labeling of thiol-modified oligonucleotides and the Cys residues of peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ7.2WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ7.2WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 7.2WS maleimide [TQ7.2WS maleimide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 90.896 µL | 454.479 µL | 908.959 µL | 4.545 mL | 9.09 mL |
5 mM | 18.179 µL | 90.896 µL | 181.792 µL | 908.959 µL | 1.818 mL |
10 mM | 9.09 µL | 45.448 µL | 90.896 µL | 454.479 µL | 908.959 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
References
View all 2 references: Citation Explorer
Visualising long distance sugar transport in fungi using infrared fluorescence scanning imaging.
Authors: Bleichrodt, Robert-Jan and Wösten, Han A B
Journal: Fungal genetics and biology : FG & B (2022): 103699
Authors: Bleichrodt, Robert-Jan and Wösten, Han A B
Journal: Fungal genetics and biology : FG & B (2022): 103699
Electrophoretic mobility shift scanning using an automated infrared DNA sequencer.
Authors: Sano, M and Ohyama, A and Takase, K and Yamamoto, M and Machida, M
Journal: BioTechniques (2001): 1056-8, 1060, 1062
Authors: Sano, M and Ohyama, A and Takase, K and Yamamoto, M and Machida, M
Journal: BioTechniques (2001): 1056-8, 1060, 1062