cAMP-NHS ester

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Physical properties

Molecular weight	684.64
Solvent	DMSO

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

684.64

Cyclic adenosine monophosphate (cAMP) is a second messenger used for intracellular signal induction. It is synthesized from adenosine triphosphate (ATP) by enzymes (g-proteins) that are attached to metabotropic receptors and become released when the receptor is activated. Cyclic AMP is involved in the regulation of glycogen, sugar, and lipid metabolism. This cAMP NHS ester is an excellent building block that can be used for developing cAMP probes and assays.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of cAMP-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	146.062 µL	730.311 µL	1.461 mL	7.303 mL	14.606 mL
5 mM	29.212 µL	146.062 µL	292.124 µL	1.461 mL	2.921 mL
10 mM	14.606 µL	73.031 µL	146.062 µL	730.311 µL	1.461 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 NHS Ester Same Structure to Alexa Fluor™ 488 NHS Ester	499	520	71000	0.92¹	0.30	0.11
XFD350 NHS Ester Same Structure to Alexa Fluor™ 350 NHS Ester	343	441	19000	-	0.25	0.19
XFD532 NHS Ester Same Structure to Alexa Fluor™ 532 NHS Ester	534	553	81000	0.61¹	0.24	0.09
XFD594 NHS Ester Same Structure to Alexa Fluor™ 594 NHS Ester	590	618	90000	0.66¹	0.43	0.56
QXY21 NHS ester [equivalent to QSY-21 NHS ester]	-	-	89000¹	-	-	0.32
XFD555 NHS Ester Same Structure to Alexa Fluor™ 555 NHS Ester	553	568	150000	0.1¹	0.08	0.08
XFD647 NHS Ester Same Structure to Alexa Fluor™ 647 NHS Ester	650	671	239000	0.33¹	0.00	0.03
XFD680 NHS Ester Same Structure to Alexa Fluor™ 680 NHS Ester	681	704	184000	0.36¹	0.00	0.05
XFD700 NHS Ester Same Structure to Alexa Fluor™ 700 NHS Ester	696	719	192000	0.25¹	0.00	0.07
XFD750 NHS Ester Same Structure to Alexa Fluor™ 750 NHS Ester	752	776	240000	0.12¹	0.00	0.04
Cy5DIGE NHS ester	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy2DIGE NHS ester	492	508	150000	0.120	0.08	0.15
Cy3DIGE NHS ester	555	569	150000¹	0.15¹	0.07	0.073
XFD546 NHS Ester Same Structure to Alexa Fluor™ 546 NHS Ester	561	572	112000	0.79¹	0.21	0.12
XFD568 NHS Ester Same Structure to Alexa Fluor™ 568 NHS Ester	579	603	91300	0.69¹	0.45	0.46
XFD514 NHS Ester Same Structure to Alexa Fluor™ 514 NHS Ester	518	543	80000	-	0.31	0.18
QXY7 NHS ester [equivalent to QSY-7 NHS ester]	-	-	90000¹	-	-	0.22
Cy3B NHS ester	560	571	120000¹	0.58¹	0.048	0.069
CypHer5E NHS Ester	643	660	-	-	-	-
CypHer7E NHS Ester	748	769	-	-	-	-
Show More (11)

Images

Figure 1. Chemical structure for cAMP-NHS ester.

Citations

View all 59 citations: Citation Explorer

Roles of Cyclic AMP Response Element Binding Activation in the ERK1/2 and p38 MAPK Signalling Pathway in Central Nervous System, Cardiovascular System, Osteoclast Differentiation and Mucin and Cytokine Production
Authors: Koga, Y., Tsurumaki, H., Aoki-Saito, H., Sato, M., Yatomi, M., Takehara, K., Hisada, T.
Journal: Int J Mol Sci (2019): ersion="1.0" encoding="UTF-8" ?>17102.enlEndN

Making and Breaking of an Essential Poison: the Cyclases and Phosphodiesterases That Produce and Degrade the Essential Second Messenger Cyclic di-AMP in Bacteria
Authors: Commichau, F. M., Heidemann, J. L., Ficner, R., Stulke, J.
Journal: J Bacteriol (2019): se name="17102.enl" path="C:\Users\aatbi\Drop

Cyclic di-AMP in host-pathogen interactions
Authors: Devaux, L., Kaminski, P. A., Trieu-Cuot, P., Firon, A.
Journal: Curr Opin Microbiol (2018): 21-28

Natural products as modulators of the cyclic-AMP pathway: evaluation and synthesis of lead compounds
Authors: Sengupta, S., Mehta, G.
Journal: Org Biomol Chem (2018): 6372-6390

Perspective of ions and messengers: an intricate link between potassium, glutamate, and cyclic di-AMP
Authors: Gundlach, J., Commichau, F. M., Stulke, J.
Journal: Curr Genet (2018): 191-195

Regulation of IP3 receptors by cyclic AMP
Authors: Taylor, C. W.
Journal: Cell Calcium (2017): 48-52

The cyclic AMP phosphodiesterase 4D5 (PDE4D5)/receptor for activated C-kinase 1 (RACK1) signalling complex as a sensor of the extracellular nano-environment
Authors: Yarwood, S. J., Parnell, E., Bird, R. J.
Journal: Cell Signal (2017): 282-289

Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA
Authors: Kato, K., Omura, H., Ishitani, R., Nureki, O.
Journal: Annu Rev Biochem (2017): 541-566

Role of cyclic AMP in the eye with glaucoma
Authors: Shim, M. S., Kim, K. Y., Ju, W. K.
Journal: BMB Rep (2017): 60-70

Regulation of Cancer Cell Responsiveness to Ionizing Radiation Treatment by Cyclic AMP Response Element Binding Nuclear Transcription Factor
Authors: D'Auria, F., Centurione, L., Centurione, M. A., Angelini, A., Di Pietro, R.
Journal: Front Oncol (2017): 76