DBCO NHS Ester

Cu-free click reaction, i.e., the strain-promoted alkyne-azide cycloaddition (SPAAC) is a bioorthogonal reaction utilizing a pair of reagents, cyclooctynes and azides that exclusively and efficiently react with each other while remain inert to naturally occurring functional groups such as hydroxy, amino and thiol groups. SPAAC enables labeling a wide variety of biomolecules without any auxiliary reagents in an aqueous and otherwise complex chemical environment through the formation of a stable triazole. Among the large number of known cyclooctynes, dibenzocyclooctyne (DBCO) compounds comprise a class of reagents that possesses reasonably fast kinetics and good stability in aqueous buffers. Within physiological temperature and pH ranges, the DBCO group will not react with thiol, amino or hydroxy groups that are naturally present in many biomolecules. DBCO NHS Ester is one of the most common reagents used for introducing DBCO group into a biomolecule via the modification of the amino groups.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
4529	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DBCO NHS Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	248.503 µL	1.243 mL	2.485 mL	12.425 mL	24.85 mL
5 mM	49.701 µL	248.503 µL	497.006 µL	2.485 mL	4.97 mL
10 mM	24.85 µL	124.251 µL	248.503 µL	1.243 mL	2.485 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	402.41 g/mol	=	mL	x	mM	=	mmol

Citations

View all 17 citations: Citation Explorer

High-sensitivity small-molecule detection of microcystin-LR cyano-toxin using a terahertz-aptamer biosensor

Authors:

Mohamed, Ahmed and Walsh, Ryan and Cherif, Mohamed and Hafez, Hassan A and Ropagnol, Xavier and Vidal, Fran{\c{c}}ois and Perreault, Jonathan and Ozaki, Tsuneyuki

Journal:

Analyst (2021): 7583--7592

The one-pot nonhydrolysis Staudinger reaction and Staudinger or SPAAC ligation

Authors:

Cheng, L., Kang, X., Wang, D., Gao, Y., Yi, L., Xi, Z.

Journal:

Org Biomol Chem (2019): 5675-5679

Optimization of Chemoenzymatic Mass Tagging by Strain-Promoted Cycloaddition (SPAAC) for the Determination of O-GlcNAc Stoichiometry by Western Blotting

Authors:

Darabedian, N., Thompson, J. W., Chuh, K. N., Hsieh-Wilson, L. C., Pratt, M. R.

Journal:

Biochemistry (2018): 5769-5774

Highly reactive bis-cyclooctyne-modified diarylethene for SPAAC-mediated cross-linking

Authors:

Strizhak, A. V., Sharma, K., Babii, O., Afonin, S., Ulrich, A. S., Komarov, I. V., Spring, D. R.

Journal:

Org Biomol Chem (2018): 8559-8564

Fused Bicyclic Caspase-1 Inhibitors Assembled by Copper-Free Strain-Promoted Alkyne-Azide Cycloaddition (SPAAC)

Authors:

Qian, L., Zhang, C. J., Wu, J., Yao, S. Q.

Journal:

Chemistry (2017): 360-369

Physical properties

Molecular weight	402.41
Solvent	DMSO

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	1353016-71-3

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 26, 2025