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AAT Bioquest

ReadiLink™ Rapid XFD647 Antibody Labeling Kit *XFD647 Same Structure to Alexa Fluor™ 647*

XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher). Readilink™ protein labeling technology is the most robust and convenient tool for preparing fluorescent antibody conjugates used for fluorescence imaging and flow cytometry applications. ReadiLink™ Rapid XFD647 Antibody Labeling Kit provides the most convenient tool for making XFD647-labeled antibody conjugates. XFD647 dye used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of XFD647 dye provided in the kit is optimized for labeling ~50 µg antibody. ReadiLink™ Rapid XFD647 Antibody Labeling Kit requires minimal hands-on time. The prepared XFD647 conjugates (with the kit) are ready to use for fluorescence imaging and flow cytometry applications without further purifications needed. It provides the most convenient method to prepare XFD647-labeled antibodies.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)Correction Factor (260 nm)
ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*491516730000.920.35-
ReadiLink™ Rapid Cy3 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*55556915000010.1510.0730.07
ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*65167025000010.271, 0.420.030.02
ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*7567792500000.30.0360.05
ReadiLink™ Rapid XFD488 Antibody Labeling Kit *XFD488 Same Structure to Alexa Fluor™ 488*499520710000.9210.110.30
ReadiLink™ Rapid XFD555 Antibody Labeling Kit *XFD555 Same Structure to Alexa Fluor™ 555*5535681500000.110.080.08
ReadiLink™ Rapid XFD594 Antibody Labeling Kit *XFD594 Same Structure to Alexa Fluor™ 594*590618900000.6610.560.43
ReadiLink™ Rapid XFD750 Antibody Labeling Kit *XFD750 Same Structure to Alexa Fluor™ 750*7527762400000.1210.040.00
ReadiLink™ Rapid FITC Antibody Labeling Kit *Production Scale*491516730000.920.35-
ReadiLink™ Rapid Cy3 Antibody Labeling Kit *Production Scale*55556915000010.1510.0730.07
ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Production Scale*65167025000010.271, 0.420.030.02
ReadiLink™ Rapid XFD488 Antibody Labeling Kit *Production Scale*499520710000.9210.110.30
ReadiLink™ Rapid XFD555 Antibody Labeling Kit *Production Scale*5535681500000.110.080.08
ReadiLink™ Rapid XFD594 Antibody Labeling Kit *Production Scale*590618900000.6610.560.43
ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Production Scale*7567792500000.30.0360.05
ReadiLink™ Rapid XFD750 Antibody Labeling Kit *Production Scale*7527762400000.1210.040.00
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References

View all 10 references: Citation Explorer
Deciphering Design Principles of Förster Resonance Energy Transfer-Based Protease Substrates: Thermolysin-Like Protease from Geobacillus stearothermophilus as a Test Case.
Authors: Ripp, Sophie and Turunen, Petri and Minot, Ethan D and Rowan, Alan E and Blank, Kerstin G
Journal: ACS omega (2018): 4148-4156
An enzymatically-sensitized sequential and concentric energy transfer relay self-assembled around semiconductor quantum dots.
Authors: Samanta, Anirban and Walper, Scott A and Susumu, Kimihiro and Dwyer, Chris L and Medintz, Igor L
Journal: Nanoscale (2015): 7603-14
Collective localized surface plasmons for high performance fluorescence biosensing.
Authors: Bauch, Martin and Dostalek, Jakub
Journal: Optics express (2013): 20470-83
Facile synthesis of symmetric, monofunctional cyanine dyes for imaging applications.
Authors: Ying, Lai-Qiang and Branchaud, Bruce P
Journal: Bioconjugate chemistry (2011): 865-9
Effects of ozone exposure during microarray posthybridization washes and scanning.
Authors: Byerly, Steve and Sundin, Kyle and Raja, Rajiv and Stanchfield, Jim and Bejjani, Bassem A and Shaffer, Lisa G
Journal: The Journal of molecular diagnostics : JMD (2009): 590-7
Page updated on November 7, 2024

Ordering information

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Catalog Number1278
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Spectral properties

Correction Factor (260 nm)

0.00

Correction Factor (280 nm)

0.03

Extinction coefficient (cm -1 M -1)

239000

Excitation (nm)

650

Emission (nm)

671

Quantum yield

0.331

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501

Components