AAT Bioquest

The Rapid & Sensitive Detection of NAD, NADH, NADP & NADPH Using Amplite™ Assay Kits

NAD, NADH, NADP and NADPH are important cofactors in cells. AAT Bioquest Amplite® NAD(P)/NAD(P)H assay kits have very low background since they are performed in a longer wavelength range that considerably reduces the interference resulted from biological samples. They have demonstrated high selectivity and enhanced sensitivity. Our kits enable the detection of NAD, NADH, NADP and NADPH by either using fluorimetric assay mode or colorimetric assay mode. These kits have been used for:
  • Studying cell metabolism
  • Measuring NAD(H) and NADP(H) in clinical research samples
  • Screening activities of enzymes (such as a variety of dehydrogenases and reductases)

Key Features of Amplite® Assay Kits:

  • Broad applications, can be used for quantifying NAD(P)/NAD(P)H in solutions and in cell extracts.
  • Sensitive, detect as low as 1 µM of NAD(P)/NAD(P)H.
  • Convenient, no wash is required.
  • Non-radioactive, no special requirements for waste treatment.

NADPH/NADP dose responses

Left: Total NAD/NADH, and their extract dose responses were measured with Amplite® NAD/NADH Ratio Assay Kit in a black 96-well plate using a Gemini microplate reader (Molecular Devices). Right: NADPH/NADP dose responses were measured with Amplite® NADPH Assay Kit in a black 96-well plate using a NOVOstar microplate reader (BMG Labtech). As low as 1 µM (100 pmol/well) of NADPH can be detected with 1 hour incubation (n=3) while there is no response from NADP.