Buccutite™ MTA, maleimide [MTAM]

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1151
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1151

Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. The method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one molecule, while FOL is added to another molecule. The cross-linking reaction is initiated by mixing Molecule-1-Buccutite ™ MTA and Molecule-2-Buccutite ™ FOL under neutral conditions. Many of our customer have requested us to offer the stand-alone Buccutite™ MTA and Buccutite™ FOL reagents to expand the application of Buccutite™crosslinking technology. Buccutite™ MTA maleimide (MTAM) can be used the same way as the widely used SMCC for crosslinking proteins. One end of the MTAM reacts (via maleimide) with thiols (-SH) of cysteine found in the reduced antibodies (by TCEP or DTT). SMCC crosslinking requires high concentration of proteins. In addition, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. Buccutite™ crosslinking reaction occurs under extremely mild and neutral conditions without any catalyst required. It is robust and efficient.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Buccutite™ MTA, maleimide [MTAM] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	86.881 µL	434.405 µL	868.81 µL	4.344 mL	8.688 mL
5 mM	17.376 µL	86.881 µL	173.762 µL	868.81 µL	1.738 mL
10 mM	8.688 µL	43.44 µL	86.881 µL	434.405 µL	868.81 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Images

Figure 1. Flow cytometry analysis of whole blood stained with PE-iFluor®610 anti-human CD8 *SK1* conjugates. Two different methods were used prepared the conjugates: the SMCC method and the Buccutite™ MTA-Maleimide method. The fluorescence signal was monitored using an Aurora spectral flow cytometer in B6-A channel. Top) Flow cytometry data was generated using a 4-laser (355 nm, 405 nm, 488 nm, and 640 nm) spectral cytometer. Bottom) CD8+ signal intensity in B6-A channel, Stain Index and Yield was compared between two methods.

Figure 2. Chemical structure for Buccutite™ MTA, maleimide [MTAM].

References

View all 50 references: Citation Explorer

DNA-protein crosslinks are repaired via homologous recombination in mammalian mitochondria.
Authors: Chesner, Lisa N and Essawy, Maram and Warner, Cecilia and Campbell, Colin
Journal: DNA repair (2021): 103026

Previously unknown type of protein crosslink discovered.
Authors: Fass, Deborah and Semenov, Sergey N
Journal: Nature (2021): 343-344

DNA-protein crosslink proteases in genome stability.
Authors: Ruggiano, Annamaria and Ramadan, Kristijan
Journal: Communications biology (2021): 11

The Trinity of SPRTN Protease Regulation.
Authors: Ruggiano, Annamaria and Ramadan, Kristijan
Journal: Trends in biochemical sciences (2021): 2-4

Protein-oligonucleotide conjugates as model substrates for DNA-protein crosslink repair proteases.
Authors: Reinking, Hannah K and Stingele, Julian
Journal: STAR protocols (2021): 100591

The Ubiquitin Ligase TRAIP: Double-Edged Sword at the Replisome.
Authors: Wu, R Alex and Pellman, David S and Walter, Johannes C
Journal: Trends in cell biology (2021): 75-85

A ubiquitin switch controls autocatalytic inactivation of the DNA-protein crosslink repair protease SPRTN.
Authors: Zhao, Shubo and Kieser, Anja and Li, Hao-Yi and Reinking, Hannah K and Weickert, Pedro and Euteneuer, Simon and Yaneva, Denitsa and Acampora, Aleida C and Götz, Maximilian J and Feederle, Regina and Stingele, Julian
Journal: Nucleic acids research (2021): 902-915

Emerging roles of Wss1 in the survival of Candida albicans under genotoxic stresses.
Authors: Homchan, Aimorn and Sukted, Juthamas and Matangkasombut, Oranart and Pakotiprapha, Danaya
Journal: Current genetics (2021): 99-105

Treatment of human cells with 5-aza-dC induces formation of PARP1-DNA covalent adducts at genomic regions targeted by DNMT1.
Authors: Kiianitsa, Kostantin and Zhang, Yinbo and Maizels, Nancy
Journal: DNA repair (2020): 102977

A Structure-Based Mechanism for DNA Entry into the Cohesin Ring.
Authors: Higashi, Torahiko L and Eickhoff, Patrik and Sousa, Joana S and Locke, Julia and Nans, Andrea and Flynn, Helen R and Snijders, Ambrosius P and Papageorgiou, George and O'Reilly, Nicola and Chen, Zhuo A and O'Reilly, Francis J and Rappsilber, Juri and Costa, Alessandro and Uhlmann, Frank
Journal: Molecular cell (2020): 917-933.e9