5-dR110 [5-Carboxy-4,7-dichlororhodamine 110]
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 544.43 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| Overview |  SDSProtocol |
See also: Cell Structures and Organelles, Nucleus, Chemical Reagents, Classic Dyes, Rhodamines and Rhodamine Derivatives, DNA and RNA Quantitation, Fluoresceins, Gene Expression Analysis & Genotyping, Genome Editing & CRISPR, Physiological Probes
Molecular weight 544.43 |
Compared to fluorescein labeling reagents such as FITC and FAM, 5-dR110 [5-carboxy-4,7-dichlororhodamine 110] reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM. 5-carboxy-4,7-dichlororhodamine (dR110) is one of the four critical 4,7-dichloro-rhodamine dyes used as the BigDye terminators for DNA sequencing in combination with d6G, dTMR and dROX. ABI PRISM® BigDye™ Terminator Cycle Sequencing is the classic method for DNA sequencing.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-dR110 [5-Carboxy-4,7-dichlororhodamine 110] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 183.678 µL | 918.392 µL | 1.837 mL | 9.184 mL | 18.368 mL |
| 5 mM | 36.736 µL | 183.678 µL | 367.357 µL | 1.837 mL | 3.674 mL |
| 10 mM | 18.368 µL | 91.839 µL | 183.678 µL | 918.392 µL | 1.837 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 5-CR110 [5-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.091 |
| 5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.214 |
| 5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4* | 552 | 578 | 90000 | 0.178 |
| 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* | 578 | 604 | 82000 | 0.168 |
| 5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2* | 544 | 570 | 100000 | 0.34 |
| 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 493 | 517 | 83000 | 0.178 |
| 5-SFX [5-FAM-X, SE] *CAS 148356-00-7* | 493 | 517 | 83000 | 0.178 |
| 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* | 491 | 516 | 73000 | 0.35 |
| 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* | 498 | 517 | 800001 | 0.35 |
Show More (3) | ||||
Images
Figure 1. Chemical structure for 5-dR110 [5-Carboxy-4,7-dichlororhodamine 110]
References
View all 8 references: Citation Explorer
Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing
Authors: Tan, L.; Liu, Y.; Yang, Q.; Li, X.; Wu, X. Y.; Gong, B.; Shen, Y. M.; Shao, Z.
Journal: Chem Commun (Camb) (2016): 954-7
Authors: Tan, L.; Liu, Y.; Yang, Q.; Li, X.; Wu, X. Y.; Gong, B.; Shen, Y. M.; Shao, Z.
Journal: Chem Commun (Camb) (2016): 954-7
Synthesis of novel tyrosinyl FRET cassettes, terminators, and their potential use in DNA sequencing
Authors: Sudhakar Rao, T.; Zhang, W.; Xiao, H.; Flick, P.; Kumar, S.; Nampalli, S.
Journal: Nucleosides Nucleotides Nucleic Acids (2003): 1443-5
Authors: Sudhakar Rao, T.; Zhang, W.; Xiao, H.; Flick, P.; Kumar, S.; Nampalli, S.
Journal: Nucleosides Nucleotides Nucleic Acids (2003): 1443-5
Improved DNA sequencing accuracy and detection of heterozygous alleles using manganese citrate and different fluorescent dye terminators.
Authors: Korch, C and Drabkin, H
Journal: Genome research (1999): 588-95
Authors: Korch, C and Drabkin, H
Journal: Genome research (1999): 588-95
Improved DNA sequencing accuracy and detection of heterozygous alleles using manganese citrate and different fluorescent dye terminators
Authors: Korch, C.; Drabkin, H.
Journal: Genome Res (1999): 588-95
Authors: Korch, C.; Drabkin, H.
Journal: Genome Res (1999): 588-95
Design strategies and performance of custom DNA sequencing primers
Authors: Buck, G. A.; Fox, J. W.; Gunthorpe, M.; Hager, K. M.; Naeve, C. W.; Pon, R. T.; Adams, P. S.; Rush, J.
Journal: Biotechniques (1999): 528-36
Authors: Buck, G. A.; Fox, J. W.; Gunthorpe, M.; Hager, K. M.; Naeve, C. W.; Pon, R. T.; Adams, P. S.; Rush, J.
Journal: Biotechniques (1999): 528-36
Peak height pattern in dichloro-rhodamine and energy transfer dye terminator sequencing
Authors: Zakeri, H.; Amparo, G.; Chen, S. M.; Spurgeon, S.; Kwok, P. Y.
Journal: Biotechniques (1998): 406-10, 412-4
Authors: Zakeri, H.; Amparo, G.; Chen, S. M.; Spurgeon, S.; Kwok, P. Y.
Journal: Biotechniques (1998): 406-10, 412-4
Peak height pattern in dichloro-rhodamine and energy transfer dye terminator sequencing.
Authors: Zakeri, H and Amparo, G and Chen, S M and Spurgeon, S and Kwok, P Y
Journal: BioTechniques (1998): 406-10, 412-4
Authors: Zakeri, H and Amparo, G and Chen, S M and Spurgeon, S and Kwok, P Y
Journal: BioTechniques (1998): 406-10, 412-4
New dye-labeled terminators for improved DNA sequencing patterns
Authors: Rosenblum, B. B.; Lee, L. G.; Spurgeon, S. L.; Khan, S. H.; Menchen, S. M.; Heiner, C. R.; Chen, S. M.
Journal: Nucleic Acids Res (1997): 4500-4
Authors: Rosenblum, B. B.; Lee, L. G.; Spurgeon, S. L.; Khan, S. H.; Menchen, S. M.; Heiner, C. R.; Chen, S. M.
Journal: Nucleic Acids Res (1997): 4500-4