Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*

Cell Meter™ Phosphatidylserine Apoptosis Assay Kit

Deep Red Fluorescence Optimized for Flow Cytometry

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. Our proprietary Apopxin™ PS sensor used in this kit is small molecule-based PS sensor. It has red fluorescence upon binding to membrane PS. This particular assay kit is optimized to monitor cell apoptosis using a flow cytometer at Cy5 channel (red fluorescence).

Detection of phosphatidylserine binding activity in Jurkat cells. Jurkat cells were treated without (Blue) or with 20 µM camptothecin (Red) in a 37 °C, 5% CO2 incubator for 4-5 hours, and then loaded with Apopxin™ Deep Red for 30 minutes. The fluorescence intensity of Apopxin™ Deep Red was measured with a FACSCalibur (Becton Dikinson) flow cytometer in FL4 channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22832	100 Tests	Price

Citations

View all 2 citations: Citation Explorer

STE20-Type Kinases MST3 and MST4 Act Non-Redundantly to Promote the Progression of Hepatocellular Carcinoma

Authors:

Caputo, Mara and Xia, Ying and Anand, Sumit Kumar and Cansby, Emmelie and Andersson, Emma and Marschall, Hanns-Ulrich and K{\"o}nigsrainer, Alfred and Peter, Andreas and Mahlapuu, Margit

Integrin $\beta$3 inhibits hypoxia-induced apoptosis in cardiomyocytes

Authors:

Su, Yifan and Tian, Hua and Wei, Lijiang and Fu, Guohui and Sun, Ting

Journal:

Acta Biochimica et Biophysica Sinica (2018): 658--665

References

View all 135 references: Citation Explorer

Evaluation of cell surface expression of phosphatidylserine in ovarian carcinoma effusions using the annexin-V/7-AAD assay: clinical relevance and comparison with other apoptosis parameters

Authors:

Dong HP, Holth A, Kleinberg L, Ruud MG, Elstr and MB, Trope CG, Davidson B, Risberg B.

Journal:

Am J Clin Pathol (2009): 756

Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis

Authors:

Wang CY, Lin YS, Su WC, Chen CL, Lin CF.

Journal:

Mol Biol Cell (2009): 4153

Trivalent methylated arsenical-induced phosphatidylserine exposure and apoptosis in platelets may lead to increased thrombus formation

Authors:

Bae ON, Lim KM, Noh JY, Chung SM, Kim SH, Chung JH.

Journal:

Toxicol Appl Pharmacol (2009): 144

Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry

Authors:

Wlodkowic D, Skommer J, Faley S, Darzynkiewicz Z, Cooper JM.

Journal:

Exp Cell Res (2009): 1706

Eurycomanone induce apoptosis in HepG2 cells via up-regulation of p53

Authors:

Zakaria Y, Rahmat A, Pihie AH, Abdullah NR, Houghton PJ.

Journal:

Cancer Cell Int (2009): 16

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	640 nm laser
Emission	660/20 nm filter
Instrument specification(s)	APC channel

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Page updated on September 25, 2025

Component A: Apopxin™ Deep Red (100X stock solution)	1 vial (200 µL/vial)
Component B: Assay Buffer (4 °C)	1 bottle (50 mL)