logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit
Orange Fluorescence Optimized for Microplate Readers
This particular kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This kit uses our proprietary orange fluorescent Apopxin™ PS sensor that specifically binds PS with affinity much higher than Annexin V (Kd < 10 nM). The PS sensor used in this kit has orange fluorescence upon binding to membrane PS. The stain has the spectral properties almost identical to those of Cy3® or Alexa Fluor® 555, making it convenient to be used for the common fluorescence instruments equipped with the light sources and filters for Cy3® or Alexa Fluor® 555 (Cy3® or Alexa Fluor® 555 are the trademarks of GE Healthcare and Invitrogen respectively). Due to its highly enhanced affinity to PS, this kit is more robust than the other commercial Annexin V-based apoptosis kits that are only used with either microscope or flow cytometry platform. This kit can be also used with a fluorescence microplate reader besides the microscope and flow cytometry platforms.
Fluorescence images of Jurkat cells in a Costar black wall/clear bottom 96-well plate stained with the Cell Meter&trade; Phosphatidylserine Apoptosis Assay Kit. Jurkat cells were treated without (Left) or with 20 &micro;M camptothecin (Right) for 5 hours. The fluorescence intensity was measured using a fluorescence microscope with&nbsp;Cy3&reg; channel.
Fluorescence images of Jurkat cells in a Costar black wall/clear bottom 96-well plate stained with the Cell Meter&trade; Phosphatidylserine Apoptosis Assay Kit. Jurkat cells were treated without (Left) or with 20 &micro;M camptothecin (Right) for 5 hours. The fluorescence intensity was measured using a fluorescence microscope with&nbsp;Cy3&reg; channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22794100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on September 25, 2025