Wheat Germ Agglutinin (WGA)

mFluor™ Violet 450-Wheat Germ Agglutinin (WGA) Conjugate

Wheat germ agglutinin (WGA) is a well-studied lectin known for its binding affinity to N-acetyl-D-glucosamine and sialic acid, making it a valuable tool in various biological applications. Its interaction with glycoconjugates enables widespread use of WGA derivatives and conjugates for fluorescence imaging and analysis, facilitating the labeling of yeast bud scars, fibrotic scar tissue, and the cell membranes of gram bacteria and mammalian cells. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer contains two identical, non-interacting binding sites complementary to 3 or 4 β-1,4-GlcNAc units. Among the monosaccharides tested, only GlcNAc shows strong binding to WGA, while ManNAc demonstrates no binding, and GalNAc exhibits weak binding. The mFluor™ Violet 450 labeled WGA is well-excited by the violet laser, emitting a bright blue fluorescence at 445 nm. Notably, the mFluor™ Violet 450 WGA conjugate retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis of various scientific investigations.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
25450	1 mg	Price

References

View all 50 references: Citation Explorer

Diverse Enterococcus faecalis strains show heterogeneity in biofilm properties.

Authors:

Schaffer, Scott D and Hutchison, Carissa A and Rouchon, Candace N and Mdluli, Nontokozo V and Weinstein, Arielle J and McDaniel, Dennis and Frank, Kristi L

Journal:

Research in microbiology (2023): 103986

Expansion Microscopy of Bacillus subtilis.

Authors:

Middelhauve, Viola and Siebrasse, Jan Peter and Kubitscheck, Ulrich

Journal:

Methods in molecular biology (Clifton, N.J.) (2023): 191-202

Human serum albumin nanoparticles as a versatile vehicle for targeted delivery of antibiotics to combat bacterial infections.

Authors:

Skoll, Katharina and Palmetzhofer, Julia and Lummerstorfer, Maria and Anzengruber, Maria and Gabor, Franz and Wirth, Michael

Journal:

Nanomedicine : nanotechnology, biology, and medicine (2023): 102685

Microencapsulation of Probiotics with Soy Protein Isolate and Alginate for the Poultry Industry.

Authors:

Babot, Jaime D and Argañaraz-Martínez, Eloy and Apella, María C and Perez Chaia, Adriana

Journal:

Food and bioprocess technology (2023): 1478-1487

Ultrasensitive Fluorescence Lateral Flow Assay for Simultaneous Detection of Pseudomonas aeruginosa and Salmonella typhimurium via Wheat Germ Agglutinin-Functionalized Magnetic Quantum Dot Nanoprobe.

Authors:

Tu, Zhijie and Yang, Xingsheng and Dong, Hao and Yu, Qing and Zheng, Shuai and Cheng, Xiaodan and Wang, Chongwen and Rong, Zhen and Wang, Shengqi

Journal:

Biosensors (2022)

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	406
Correction factor (260 nm)	0.338
Correction factor (280 nm)	0.078
Extinction coefficient (cm ^-1 M ^-1)	35000 ¹
Excitation (nm)	406
Emission (nm)	445
Quantum yield	0.81 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	406 nm
Emission	445 nm
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 8, 2024