iFluor® 700-Wheat Germ Agglutinin (WGA) Conjugate
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Solvent | Water |
Correction Factor (260 nm) | 0.09 |
Correction Factor (280 nm) | 0.04 |
Correction Factor (565 nm) | 0.0766 |
Correction Factor (650 nm) | 0.4004 |
Extinction coefficient (cm -1 M -1) | 2200001 |
Excitation (nm) | 690 |
Emission (nm) | 713 |
Quantum yield | 0.231 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Correction Factor (260 nm) 0.09 | Correction Factor (280 nm) 0.04 | Correction Factor (565 nm) 0.0766 | Correction Factor (650 nm) 0.4004 | Extinction coefficient (cm -1 M -1) 2200001 | Excitation (nm) 690 | Emission (nm) 713 | Quantum yield 0.231 |
Platform
Fluorescence microscope
Excitation | Cy5 filter set |
Emission | Cy5 filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 500 µL of ddH2O into the powder form to make a 2 mg/mL stock solution.
Note: The reconstituted conjugate solution can be stored at 2-8 °C for short-term storage or at -20 °C for long-term storage.
PREPARATION OF WORKING SOLUTION
Add 5 µL of 200X WGA conjugate solution to 1 mL HHBS Buffer.
Note: The optimized staining concentration may be different with different cell lines. The recommended starting concentration is 5-10 µg/mL for live cells.
SAMPLE EXPERIMENTAL PROTOCOL
Warm the vial to room temperature centrifuge briefly before opening. Staining protocols vary with applications. Appropriate dilution of conjugates should be determined experimentally.
- Wash cells twice with a HHBS buffer.
Add 100 µL iFluor® 700-WGA working solution.
- Incubate cells with WGA working solution for 10-30 minutes at 37 °C.
- Wash cells twice with HHBS buffer.
Image cells on a fluorescence microscope using Cy5 filter set.
WGA conjugates can be also used to stain fixed cells.
Fix cells with 4% Formaldehyde in PBS.
Note: For fixed cell membrane staining, it is recommended to stain without the permeabilization step. A permeabilization step after fixation can facilitate staining intracellular compartments such as Golgi and Endoplasmic Reticulum (ER) structures.
Add 100 µL iFluor® 700-WGA working solution.
- Incubate cells with WGA working solution for 10-30 minutes at room temperature.
- Wash cells twice with HHBS buffer.
Image cells on a fluorescence microscope using Cy5 filter set.
Spectrum

Spectral properties
Correction Factor (260 nm) | 0.09 |
Correction Factor (280 nm) | 0.04 |
Correction Factor (565 nm) | 0.0766 |
Correction Factor (650 nm) | 0.4004 |
Extinction coefficient (cm -1 M -1) | 2200001 |
Excitation (nm) | 690 |
Emission (nm) | 713 |
Quantum yield | 0.231 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 488-Wheat Germ Agglutinin (WGA) Conjugate | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555-Wheat Germ Agglutinin (WGA) Conjugate | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 594-Wheat Germ Agglutinin (WGA) Conjugate | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
iFluor® 647-Wheat Germ Agglutinin (WGA) Conjugate | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 350-Wheat Germ Agglutinin (WGA) Conjugate | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
iFluor® 680-Wheat Germ Agglutinin (WGA) Conjugate | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 750-Wheat Germ Agglutinin (WGA) Conjugate | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
iFluor® 790-Wheat Germ Agglutinin (WGA) Conjugate | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
Images
References
Authors: Qin, Mengyuan and Huang, Jie and Zhong, Jincheng and Zhang, Yingxian and Tong, Shen and Cheng, Hui and Deng, Xiangquan and Zheng, Lei and Zhang, Wanjian and Qiu, Ping and Wang, Ke
Journal: Journal of biophotonics (2023): e202200365
Authors: Golub, Oggie and Kilgore, Jason A and Dolman, Nick J
Journal: Current protocols (2023): e754
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Journal: Methods in molecular biology (Clifton, N.J.) (2023): 191-202
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Journal: Journal for immunotherapy of cancer (2023)
Authors: Klawonn, Isabell and Dunker, Susanne and Kagami, Maiko and Grossart, Hans-Peter and Van den Wyngaert, Silke
Journal: Microbial ecology (2023): 9-23
Authors: Anzengruber, Maria and Wimmer, Lukas and Szuchar, Raffaela and Skoll, Katharina and Wirth, Michael and Gabor, Franz
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2023): 106330
Authors: Pena-Passos, Matheus and Sisti, Laís Soêmis and Mayer, Juliana Lischka Sampaio
Journal: Journal of visualized experiments : JoVE (2022)
Authors: Montero, Héctor and Paszkowski, Uta
Journal: The Plant journal : for cell and molecular biology (2022): 294-301