iFluor® 488-Wheat Germ Agglutinin (WGA) Conjugate
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Solvent | Water |
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.91 |
Platform
Fluorescence microscope
Excitation | FITC filter set |
Emission | FITC filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 500 µL of ddH2O into the powder form to make a 2 mg/mL stock solution.
Note: The reconstituted conjugate solution can be stored at 2-8 °C for short-term storage or at -20 °C for long-term storage.
PREPARATION OF WORKING SOLUTION
Add 5 µL of 200X WGA conjugate solution to 1 mL HHBS Buffer.
Note: The optimized staining concentration may be different with different cell lines. The recommended starting concentration is 5-10 µg/mL for live cells.
SAMPLE EXPERIMENTAL PROTOCOL
Warm the vial to room temperature centrifuge briefly before opening. Staining protocols vary with applications. Appropriate dilution of conjugates should be determined experimentally.
- Wash cells twice with a HHBS buffer.
Add 100 µL iFluor® 488-WGA working solution.
- Incubate cells with WGA working solution for 10-30 minutes at 37 °C.
- Wash cells twice with HHBS buffer.
- Image cells on a fluorescence microscope using FITC filter set.
WGA conjugates can be also used to stain fixed cells.
Fix cells with 4% Formaldehyde in PBS.
Note: For fixed cell membrane staining, it is recommended to stain without the permeabilization step. A permeabilization step after fixation can facilitate staining intracellular compartments such as Golgi and Endoplasmic Reticulum (ER) structures.
Add 100 µL iFluor® 488-WGA working solution.
- Incubate cells with WGA working solution for 10-30 minutes at room temperature.
- Wash cells twice with HHBS buffer.
- Image cells on a fluorescence microscope using FITC filter set.
Spectrum

Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 555-Wheat Germ Agglutinin (WGA) Conjugate | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 594-Wheat Germ Agglutinin (WGA) Conjugate | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
iFluor® 647-Wheat Germ Agglutinin (WGA) Conjugate | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 350-Wheat Germ Agglutinin (WGA) Conjugate | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
iFluor® 680-Wheat Germ Agglutinin (WGA) Conjugate | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 700-Wheat Germ Agglutinin (WGA) Conjugate | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 750-Wheat Germ Agglutinin (WGA) Conjugate | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
iFluor® 790-Wheat Germ Agglutinin (WGA) Conjugate | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
Images
Citations
Authors: Li, Zhi and Zhang, Quanbin and Sun, Yuan-yuan and Wu, Ning
Journal: Heliyon (2023)
Authors: Yan, Meiling and Li, Yun and Luo, Qingmao and Zeng, Wenru and Shao, Xiaoqi and Li, Lun and Wang, Qing and Wang, Dongwei and Zhang, Yue and Diao, Hongtao and others,
Journal: Cell death discovery (2022): 1--12
Authors: Xia, Zongyi and Chen, Bing and Zhou, Chi and Wang, Yitian and Ren, Jinyang and Yao, Xujin and Wan, Qi and Lian, Zhexun
Journal: (2022)
References
Authors: Avalbaev, Azamat and Bezrukova, Marina and Allagulova, Chulpan and Lubyanova, Alsu and Kudoyarova, Guzel and Fedorova, Kristina and Maslennikova, Dilara and Yuldashev, Ruslan and Shakirova, Farida
Journal: Plant physiology and biochemistry : PPB (2020): 420-427
Authors: Janković, Tamara and Goč, Sanja and Mitić, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava
Journal: Upsala journal of medical sciences (2020): 10-18
Authors: Guevara, Rebekah B and Fox, Barbara A and Bzik, David J
Journal: mSphere (2020)
Authors: Killilea, David W and McQueen, Rebecca and Abegania, Judi R
Journal: Journal of food science (2020): 808-815
Authors: Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu
Journal: Colloids and surfaces. B, Biointerfaces (2020): 110572
Authors: Zhang, Lejie and Zhang, Mei and Bellve, Karl and Fogarty, Kevin E and Castro, Maite A and Brauchi, Sebastian and Kobertz, William R
Journal: The Journal of general physiology (2020)
Authors: Leyva, Eduardo and Medrano-Cerano, Jorge L and Cano-Sánchez, Patricia and López-González, Itzel and Gómez-Velasco, Homero and Del Río-Portilla, Federico and García-Hernández, Enrique
Journal: Biopolymers (2019): e23242
Authors: Kuo, Yung-Chih and Chang, Yu-Hsuan and Rajesh, Rajendiran
Journal: Materials science & engineering. C, Materials for biological applications (2019): 114-128
Authors: Ryva, Bradley and Zhang, Keman and Asthana, Abhishek and Wong, Derek and Vicioso, Yorleny and Parameswaran, Reshmi
Journal: Frontiers in oncology (2019): 100
Authors: Wang, Ruoning and Huang, Jinyu and Chen, Jian and Yang, Mengmeng and Wang, Honglan and Qiao, Hongzhi and Chen, Zhipeng and Hu, Lihong and Di, Liuqing and Li, Junsong
Journal: Nanomedicine : nanotechnology, biology, and medicine (2019): 102068
Application notes
FAQ
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