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mFluor™ Violet 540-Wheat Germ Agglutinin (WGA) Conjugate

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Physical properties
Spectral properties
Absorbance (nm)401
Correction Factor (260 nm)1.326
Correction Factor (280 nm)0.543
Extinction coefficient (cm -1 M -1)180001
Excitation (nm)402
Emission (nm)535
Quantum yield0.211
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Absorbance (nm)
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
Wheat germ agglutinin (WGA) is a well-studied lectin known for its binding affinity to N-acetyl-D-glucosamine and sialic acid, making it a valuable tool in various biological applications. Its interaction with glycoconjugates enables widespread use of WGA derivatives and conjugates for fluorescence imaging and analysis, facilitating the labeling of yeast bud scars, fibrotic scar tissue, and the cell membranes of gram bacteria and mammalian cells. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer contains two identical, non-interacting binding sites complementary to 3 or 4 β-1,4-GlcNAc units. Among the monosaccharides tested, only GlcNAc shows strong binding to WGA, while ManNAc demonstrates no binding, and GalNAc exhibits weak binding. The mFluor™ Violet 540 labeled WGA is well-excited by the violet laser, emitting a bright green fluorescence at 535 nm. Notably, the mFluor™ Violet 540 WGA conjugate retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis of various scientific investigations.


Fluorescence microscope

Excitation402 nm
Emission535 nm
Recommended plateBlack wall/clear bottom

Example protocol


Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles

mFluor™ Violet 540-Wheat Germ Agglutinin (WGA) Conjugate stock solution (200X)

Add 500 µL of ddH2O into the powder form to make a 2 mg/mL stock solution.

Note: The reconstituted conjugate solution can be stored at 2-8 °C for short-term storage or at -20 °C for long-term storage.


mFluor™ Violet 540-Wheat Germ Agglutinin (WGA) Conjugate working solution (1X)

Add 5 µL of 200X WGA conjugate solution to 1 mL HHBS Buffer.

Note: The optimized staining concentration may be different with different cell lines. The recommended starting concentration is 5-10 µg/mL for live cells.


Warm the vial to room temperature centrifuge briefly before opening. Staining protocols vary with applications. Appropriate dilution of conjugates should be determined experimentally.

Live Cells Stain
  1. Wash cells twice with a HHBS buffer.
  2. Add 100 µL mFluor™ Violet 540-WGA working solution.

  3. Incubate cells with WGA working solution for 10-30 minutes at 37 °C.
  4. Wash cells twice with HHBS buffer.
  5. Image cells on a fluorescence microscope using Ex/Em = 402/535 nm.

Fixed Cells Stain

WGA conjugates can be also used to stain fixed cells.

  1. Fix cells with 4% Formaldehyde in PBS.

    Note: For fixed cell membrane staining, it is recommended to stain without the permeabilization step. A permeabilization step after fixation can facilitate staining intracellular compartments such as Golgi and Endoplasmic Reticulum (ER) structures.

  2. Add 100 µL mFluor™ Violet 540-WGA working solution.

  3. Incubate cells with WGA working solution for 10-30 minutes at room temperature.
  4. Wash cells twice with HHBS buffer.
  5. Image cells on a fluorescence microscope using Ex/Em = 402/535 nm.


Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)401
Correction Factor (260 nm)1.326
Correction Factor (280 nm)0.543
Extinction coefficient (cm -1 M -1)180001
Excitation (nm)402
Emission (nm)535
Quantum yield0.211

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 450-Wheat Germ Agglutinin (WGA) Conjugate4064453500010.8110.3380.078
mFluor™ Violet 500-Wheat Germ Agglutinin (WGA) Conjugate4105012500010.8110.7690.365


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