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PE-iFluor® 597 Tandem
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Solvent | Water |
Spectral properties
| Correction Factor (280 nm) | 0.3 |
| Excitation (nm) | 565 |
| Emission (nm) | 612 |
| Quantum yield | 1960000 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
| Overview |  SDSProtocol |
Correction Factor (280 nm) 0.3 | Excitation (nm) 565 | Emission (nm) 612 | Quantum yield 1960000 |
Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 597 is a superior replacement to the commonly used PE-Texas Red tandem with significantly improved FRET efficiency and signal/background ratio. Its primary absorption peak is at 565 nm with emission peak around 610 nm. AAT Bioquest also offers a unique preactivated PE-iFluor® to facilitate the PE- iFluor® 597 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. PE-iFluor® 597 has emission of ~614 nm with superior signal compared to other spectrally similar PE tandems.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.3 |
| Excitation (nm) | 565 |
| Emission (nm) | 612 |
| Quantum yield | 1960000 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| PE-iFluor® 700 Tandem | 565 | 708 | 1960000 |
| PE-iFluor® 610 Tandem | 565 | 625 | 1960000 |
| PE-iFluor® 647 Tandem | 565 | 666 | 1960000 |
| PE-iFluor® 750 Tandem | 565 | 778 | 1960000 |
| PE-iFluor® 710 Tandem | 565 | 747 | 1960000 |
| PE-iFluor® 594 Tandem | 565 | 606 | 1960000 |
| PE-iFluor® 660 Tandem | 565 | 695 | 1960000 |
| PE-iFluor® 780 Tandem | 566 | 575 | 1960000 |
| PE-iFluor® 740 Tandem | 565 | 767 | 1960000 |
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Images
Figure 1. Flow cytometry analysis of whole blood cells stained with PE-iFluor® 597 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 597 specific B6-A channel.
References
View all 4 references: Citation Explorer
Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.
Authors: Genkel, Vadim and Dolgushin, Ilya and Baturina, Irina and Savochkina, Albina and Kuznetsova, Alla and Pykhova, Lubov and Shaposhnik, Igor
Journal: International journal of inflammation (2021): 6695468
Authors: Genkel, Vadim and Dolgushin, Ilya and Baturina, Irina and Savochkina, Albina and Kuznetsova, Alla and Pykhova, Lubov and Shaposhnik, Igor
Journal: International journal of inflammation (2021): 6695468
Fluorochrome choices for multi-color flow cytometry.
Authors: Flores-Montero, Juan and Kalina, Tomas and Corral-Mateos, Alba and Sanoja-Flores, Luzalba and Pérez-Andrés, Martin and Martin-Ayuso, Marta and Sedek, Lukasz and Rejlova, Katerina and Mayado, Andrea and Fernández, Paula and van der Velden, Vincent and Bottcher, Sebastian and van Dongen, Jaques J M and Orfao, Alberto
Journal: Journal of immunological methods (2019): 112618
Authors: Flores-Montero, Juan and Kalina, Tomas and Corral-Mateos, Alba and Sanoja-Flores, Luzalba and Pérez-Andrés, Martin and Martin-Ayuso, Marta and Sedek, Lukasz and Rejlova, Katerina and Mayado, Andrea and Fernández, Paula and van der Velden, Vincent and Bottcher, Sebastian and van Dongen, Jaques J M and Orfao, Alberto
Journal: Journal of immunological methods (2019): 112618
Unexpected interference in cell surface staining by monoclonal antibodies to unrelated antigens.
Authors: De Vita, Martina and Catzola, Valentina and Buzzonetti, Alexia and Fossati, Marco and Battaglia, Alessandra and Zamai, Loris and Fattorossi, Andrea
Journal: Cytometry. Part B, Clinical cytometry (2015): 352-4
Authors: De Vita, Martina and Catzola, Valentina and Buzzonetti, Alexia and Fossati, Marco and Battaglia, Alessandra and Zamai, Loris and Fattorossi, Andrea
Journal: Cytometry. Part B, Clinical cytometry (2015): 352-4
Unexpected interference in cell surface staining by monoclonal antibodies to unrelated antigens.
Authors: De Vita, Martina and Catzola, Valentina and Buzzonetti, Alexia and Fossati, Marco and Battaglia, Alessandra and Zamai, Loris and Fattorossi, Andrea
Journal: Cytometry. Part B, Clinical cytometry (2014)
Authors: De Vita, Martina and Catzola, Valentina and Buzzonetti, Alexia and Fossati, Marco and Battaglia, Alessandra and Zamai, Loris and Fattorossi, Andrea
Journal: Cytometry. Part B, Clinical cytometry (2014)