logo
AAT Bioquest

PE-iFluor™ 597 Tandem

Flow cytometry analysis of whole blood cells stained with PE-iFluor® 597 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the PE-iFluor® 597 specific B6-A channel.
Flow cytometry analysis of whole blood cells stained with PE-iFluor® 597 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the PE-iFluor® 597 specific B6-A channel.
Flow cytometry analysis of whole blood cells stained with PE-iFluor® 597 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the PE-iFluor® 597 specific B6-A channel.
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
QuotationRequest
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventWater
Spectral properties
Correction Factor (280 nm)0.3
Excitation (nm)565
Emission (nm)612
Quantum yield1960000
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Correction Factor (280 nm)
0.3
Excitation (nm)
565
Emission (nm)
612
Quantum yield
1960000
Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor™ 594 or iFluor™ 750). PE-iFluor™ 597 is a superior replacement to the commonly used PE-Texas Red tandem with significantly improved FRET efficiency and signal/background ratio. Its primary absorption peak is at 565 nm with emission peak around 610 nm. AAT Bioquest also offers a unique preactivated PE-iFluor™ 597 to facilitate the PE- iFluor™ 597 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. PE-iFluor™ 597 has emission of ~614 nm with superior signal compared to other spectrally similar PE tandems.

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (280 nm)0.3
Excitation (nm)565
Emission (nm)612
Quantum yield1960000

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
PE-iFluor® 700 Tandem5657081960000
PE-iFluor® 610 Tandem5656251960000
PE-iFluor® 647 Tandem5656661960000
PE-iFluor® 750 Tandem5657781960000
PE-iFluor® 710 Tandem5657471960000
PE-iFluor® 594 Tandem5656061960000
PE-iFluor® 660 Tandem5656951960000
PE-iFluor™ 740 Tandem5657671960000
PE-iFluor™ 720 Tandem5657501960000
PE-iFluor™ 770 Tandem5657951960000
Show More (1)

Images


References


View all 4 references: Citation Explorer
Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.
Authors: Genkel, Vadim and Dolgushin, Ilya and Baturina, Irina and Savochkina, Albina and Kuznetsova, Alla and Pykhova, Lubov and Shaposhnik, Igor
Journal: International journal of inflammation (2021): 6695468
Fluorochrome choices for multi-color flow cytometry.
Authors: Flores-Montero, Juan and Kalina, Tomas and Corral-Mateos, Alba and Sanoja-Flores, Luzalba and Pérez-Andrés, Martin and Martin-Ayuso, Marta and Sedek, Lukasz and Rejlova, Katerina and Mayado, Andrea and Fernández, Paula and van der Velden, Vincent and Bottcher, Sebastian and van Dongen, Jaques J M and Orfao, Alberto
Journal: Journal of immunological methods (2019): 112618
Unexpected interference in cell surface staining by monoclonal antibodies to unrelated antigens.
Authors: De Vita, Martina and Catzola, Valentina and Buzzonetti, Alexia and Fossati, Marco and Battaglia, Alessandra and Zamai, Loris and Fattorossi, Andrea
Journal: Cytometry. Part B, Clinical cytometry (2015): 352-4
Unexpected interference in cell surface staining by monoclonal antibodies to unrelated antigens.
Authors: De Vita, Martina and Catzola, Valentina and Buzzonetti, Alexia and Fossati, Marco and Battaglia, Alessandra and Zamai, Loris and Fattorossi, Andrea
Journal: Cytometry. Part B, Clinical cytometry (2014)