AAT Bioquest

PE-iFluor® 660 Tandem

Flow cytometry analysis of PBMC stained with PE-iFlour® 660 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 660 specific B9-A channel.
Flow cytometry analysis of PBMC stained with PE-iFlour® 660 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 660 specific B9-A channel.
Flow cytometry analysis of PBMC stained with PE-iFlour® 660 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 660 specific B9-A channel.
The fluorescence intensity of PE-iFluor® 660 anti-human CD4 *SK3* conjugate at different concentrations in the range of 0.03125 to 0.5 µg. Results showed that the fluorescence intensity of the CD conjugates remained nearly consistent.
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Physical properties
Spectral properties
Absorbance (nm)566
Extinction coefficient (cm -1 M -1)1960000
Excitation (nm)565
Emission (nm)695
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure


Absorbance (nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 660 is a new unique tandem color for multicolor applications, in particular, for designing spectral flow cytometric panels. It has been validated with Cytek’s Aurora flow cytometer. AAT Bioquest also offers a unique preactivated PE-iFluor® 660 to facilitate the PE-iFluor® 660 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE- iFluor® 660 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry.


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Spectral properties

Absorbance (nm)566
Extinction coefficient (cm -1 M -1)1960000
Excitation (nm)565
Emission (nm)695

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
PE-iFluor® 700 Tandem5657081960000
PE-iFluor® 610 Tandem5656251960000
PE-iFluor® 647 Tandem5656661960000
PE-iFluor® 750 Tandem5657781960000
PE-iFluor® 710 Tandem5657471960000
PE-iFluor® 594 Tandem5656061960000
PE-iFluor® 780 Tandem5665751960000
PE-iFluor® 597 Tandem565612-
PE-iFluor® 740 Tandem5657671960000
PE-iFluor® 720 Tandem5657501960000
PE-iFluor® 770 Tandem5675751960000
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