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PE-iFluor® 660 Tandem
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Application notes
Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 660 is a new unique tandem color for multicolor applications, in particular, for designing spectral flow cytometric panels. It has been validated with Cytek’s Aurora flow cytometer. AAT Bioquest also offers a unique preactivated PE-iFluor® 660 to facilitate the PE-iFluor® 660 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE- iFluor® 660 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| PE-iFluor® 700 Tandem | 565 | 708 | 1960000 |
| PE-iFluor® 610 Tandem | 565 | 625 | 1960000 |
| PE-iFluor® 647 Tandem | 565 | 666 | 1960000 |
| PE-iFluor® 750 Tandem | 565 | 778 | 1960000 |
| PE-iFluor® 710 Tandem | 565 | 747 | 1960000 |
| PE-iFluor® 594 Tandem | 565 | 606 | 1960000 |
| PE-iFluor® 780 Tandem | 566 | 575 | 1960000 |
| PE-iFluor® 597 Tandem | 565 | 612 | - |
| PE-iFluor® 740 Tandem | 565 | 767 | 1960000 |
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References
View all 35 references: Citation Explorer
Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia.
Authors: Kreutmair, Stefanie and Unger, Susanne and Núñez, Nicolás Gonzalo and Ingelfinger, Florian and Alberti, Chiara and De Feo, Donatella and Krishnarajah, Sinduya and Kauffmann, Manuel and Friebel, Ekaterina and Babaei, Sepideh and Gaborit, Benjamin and Lutz, Mirjam and Jurado, Nicole Puertas and Malek, Nisar P and Goepel, Siri and Rosenberger, Peter and Häberle, Helene A and Ayoub, Ikram and Al-Hajj, Sally and Nilsson, Jakob and Claassen, Manfred and Liblau, Roland and Martin-Blondel, Guillaume and Bitzer, Michael and Roquilly, Antoine and Becher, Burkhard
Journal: Immunity (2021): 1578-1593.e5
Authors: Kreutmair, Stefanie and Unger, Susanne and Núñez, Nicolás Gonzalo and Ingelfinger, Florian and Alberti, Chiara and De Feo, Donatella and Krishnarajah, Sinduya and Kauffmann, Manuel and Friebel, Ekaterina and Babaei, Sepideh and Gaborit, Benjamin and Lutz, Mirjam and Jurado, Nicole Puertas and Malek, Nisar P and Goepel, Siri and Rosenberger, Peter and Häberle, Helene A and Ayoub, Ikram and Al-Hajj, Sally and Nilsson, Jakob and Claassen, Manfred and Liblau, Roland and Martin-Blondel, Guillaume and Bitzer, Michael and Roquilly, Antoine and Becher, Burkhard
Journal: Immunity (2021): 1578-1593.e5
Protocol for Creating Antibodies with Complex Fluorescence Spectra.
Authors: McCarthy, Madeline E and Anglin, Caitlin M and Peer, Heather A and Boleman, Sevanna A and Klaubert, Stephanie R and Birtwistle, Marc R
Journal: Bioconjugate chemistry (2021): 1156-1166
Authors: McCarthy, Madeline E and Anglin, Caitlin M and Peer, Heather A and Boleman, Sevanna A and Klaubert, Stephanie R and Birtwistle, Marc R
Journal: Bioconjugate chemistry (2021): 1156-1166
Methotrexate Hampers Immunogenicity to BNT162b2 mRNA COVID-19 Vaccine in Immune-Mediated Inflammatory Disease.
Authors: Haberman, Rebecca H and Herati, Ramin Sedaghat and Simon, David and Samanovic, Marie and Blank, Rebecca B and Tuen, Michael and Koralov, Sergei B and Atreya, Raja and Tascilar, Koray and Allen, Joseph R and Castillo, Rochelle and Cornelius, Amber R and Rackoff, Paula and Solomon, Gary and Adhikari, Samrachana and Azar, Natalie and Rosenthal, Pamela and Izmirly, Peter and Samuels, Jonathan and Golden, Brian and Reddy, Soumya and Neurath, Markus and Abramson, Steven B and Schett, Georg and Mulligan, Mark J and Scher, Jose U
Journal: medRxiv : the preprint server for health sciences (2021)
Authors: Haberman, Rebecca H and Herati, Ramin Sedaghat and Simon, David and Samanovic, Marie and Blank, Rebecca B and Tuen, Michael and Koralov, Sergei B and Atreya, Raja and Tascilar, Koray and Allen, Joseph R and Castillo, Rochelle and Cornelius, Amber R and Rackoff, Paula and Solomon, Gary and Adhikari, Samrachana and Azar, Natalie and Rosenthal, Pamela and Izmirly, Peter and Samuels, Jonathan and Golden, Brian and Reddy, Soumya and Neurath, Markus and Abramson, Steven B and Schett, Georg and Mulligan, Mark J and Scher, Jose U
Journal: medRxiv : the preprint server for health sciences (2021)
Normal Numbers of Stem Cell Memory T Cells Despite Strongly Reduced Naive T Cells Support Intact Memory T Cell Compartment in Ataxia Telangiectasia.
Authors: Weitering, Thomas J and Melsen, Janine E and van Ostaijen-Ten Dam, Monique M and Weemaes, Corry M R and Schilham, Marco W and van der Burg, Mirjam
Journal: Frontiers in immunology (2021): 686333
Authors: Weitering, Thomas J and Melsen, Janine E and van Ostaijen-Ten Dam, Monique M and Weemaes, Corry M R and Schilham, Marco W and van der Burg, Mirjam
Journal: Frontiers in immunology (2021): 686333
Human Lung-Resident Macrophages Colocalize with and Provide Costimulation to PD1hi Tissue-Resident Memory T Cells.
Authors: Snyder, Mark E and Sembrat, John and Noda, Kentaro and Myerburg, Michael M and Craig, Andrew and Mitash, Nilay and Harano, Takashi and Furukawa, Masashi and Pilewski, Joseph and McDyer, John and Rojas, Mauricio and Sanchez, Pablo
Journal: American journal of respiratory and critical care medicine (2021): 1230-1244
Authors: Snyder, Mark E and Sembrat, John and Noda, Kentaro and Myerburg, Michael M and Craig, Andrew and Mitash, Nilay and Harano, Takashi and Furukawa, Masashi and Pilewski, Joseph and McDyer, John and Rojas, Mauricio and Sanchez, Pablo
Journal: American journal of respiratory and critical care medicine (2021): 1230-1244