PE-iFluor® 660 Tandem

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	695

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Absorbance (nm)

566

Extinction coefficient (cm ^-1 M ^-1)

1960000

Excitation (nm)

565

Emission (nm)

695

Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 660 is a new unique tandem color for multicolor applications, in particular, for designing spectral flow cytometric panels. It has been validated with Cytek’s Aurora flow cytometer. AAT Bioquest also offers a unique preactivated PE-iFluor® 660 to facilitate the PE-iFluor® 660 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE- iFluor® 660 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	695

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
PE-iFluor® 700 Tandem	565	708	1960000
PE-iFluor® 610 Tandem	565	625	1960000
PE-iFluor® 647 Tandem	565	666	1960000
PE-iFluor® 750 Tandem	565	778	1960000
PE-iFluor® 710 Tandem	565	747	1960000
PE-iFluor® 594 Tandem	565	606	1960000
PE-iFluor® 780 Tandem	566	575	1960000
PE-iFluor® 597 Tandem	565	612	-
PE-iFluor® 740 Tandem	565	767	1960000
PE-iFluor® 720 Tandem	565	750	1960000
PE-iFluor® 770 Tandem	567	575	1960000
Show More (2)

Images

Figure 1. Flow cytometry analysis of PBMC stained with PE-iFlour® 660 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 660 specific B9-A channel.

Figure 2. The fluorescence intensity of PE-iFluor® 660 anti-human CD4 *SK3* conjugate at different concentrations in the range of 0.03125 to 0.5 µg. Results showed that the fluorescence intensity of the CD conjugates remained nearly consistent.

References

View all 35 references: Citation Explorer

Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia.
Authors: Kreutmair, Stefanie and Unger, Susanne and Núñez, Nicolás Gonzalo and Ingelfinger, Florian and Alberti, Chiara and De Feo, Donatella and Krishnarajah, Sinduya and Kauffmann, Manuel and Friebel, Ekaterina and Babaei, Sepideh and Gaborit, Benjamin and Lutz, Mirjam and Jurado, Nicole Puertas and Malek, Nisar P and Goepel, Siri and Rosenberger, Peter and Häberle, Helene A and Ayoub, Ikram and Al-Hajj, Sally and Nilsson, Jakob and Claassen, Manfred and Liblau, Roland and Martin-Blondel, Guillaume and Bitzer, Michael and Roquilly, Antoine and Becher, Burkhard
Journal: Immunity (2021): 1578-1593.e5

Use of Toll-Like Receptor (TLR) Ligation to Characterize Human Regulatory B-Cells Subsets.
Authors: Chayé, Mathilde A M and Tontini, Chiara and Ozir-Fazalalikhan, Arifa and Voskamp, Astrid L and Smits, Hermelijn H
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 235-261

Distinct cellular immune profiles in the airways and blood of critically ill patients with COVID-19.
Authors: Saris, Anno and Reijnders, Tom Dy and Nossent, Esther J and Schuurman, Alex R and Verhoeff, Jan and Asten, Saskia van and Bontkes, Hetty and Blok, Siebe and Duitman, Janwillem and Bogaard, Harm-Jan and Heunks, Leo and Lutter, Rene and van der Poll, Tom and Garcia Vallejo, Juan J and ,
Journal: Thorax (2021)

Methotrexate hampers immunogenicity to BNT162b2 mRNA COVID-19 vaccine in immune-mediated inflammatory disease.
Authors: Haberman, Rebecca H and Herati, Ramin and Simon, David and Samanovic, Marie and Blank, Rebecca B and Tuen, Michael and Koralov, Sergei B and Atreya, Raja and Tascilar, Koray and Allen, Joseph R and Castillo, Rochelle and Cornelius, Amber R and Rackoff, Paula and Solomon, Gary and Adhikari, Samrachana and Azar, Natalie and Rosenthal, Pamela and Izmirly, Peter and Samuels, Jonathan and Golden, Brian and Reddy, Soumya M and Neurath, Markus F and Abramson, Steven B and Schett, Georg and Mulligan, Mark J and Scher, Jose U
Journal: Annals of the rheumatic diseases (2021)

Basophils promote barrier dysfunction and resolution in the atopic skin.
Authors: Pellefigues, Christophe and Naidoo, Karmella and Mehta, Palak and Schmidt, Alfonso J and Jagot, Ferdinand and Roussel, Elsa and Cait, Alissa and Yumnam, Bibek and Chappell, Sally and Meijlink, Kimberley and Camberis, Mali and Jiang, Jean X and Painter, Gavin and Filbey, Kara and Uluçkan, Özge and Gasser, Olivier and Le Gros, Graham
Journal: The Journal of allergy and clinical immunology (2021)

IFNγ-producing CD8+ tissue resident memory T cells are a targetable hallmark of immune checkpoint inhibitor-colitis.
Authors: Sasson, Sarah C and Slevin, Stephanie M and Cheung, Vincent Tf and Nassiri, Isar and Olsson-Brown, Anna and Fryer, Eve and Ferreira, Ricardo C and Trzupek, Dominik and Gupta, Tarun and Al-Hillawi, Lulia and Issaias, Mari-Lenna and Easton, Alistair and Campo, Leticia and FitzPatrick, Michael Eb and Adams, Joss and Chitnis, Meenali and Protheroe, Andrew and Tuthill, Mark and Coupe, Nicholas and Simmons, Alison and Payne, Miranda and Middleton, Mark R and Travis, Simon Pl and , and Fairfax, Benjamin P and Klenerman, Paul and Brain, Oliver
Journal: Gastroenterology (2021)

Multibatch Cytometry Data Integration for Optimal Immunophenotyping.
Authors: Ogishi, Masato and Yang, Rui and Gruber, Conor and Zhang, Peng and Pelham, Simon J and Spaan, András N and Rosain, Jérémie and Chbihi, Marwa and Han, Ji Eun and Rao, V Koneti and Kainulainen, Leena and Bustamante, Jacinta and Boisson, Bertrand and Bogunovic, Dusan and Boisson-Dupuis, Stéphanie and Casanova, Jean-Laurent
Journal: Journal of immunology (Baltimore, Md. : 1950) (2021): 206-213

Detecting Förster resonance energy transfer in living cells by conventional and spectral flow cytometry.
Authors: Henderson, Jared and Havranek, Ondrej and Ma, Man Chun John and Herman, Vaclav and Kupcova, Kristyna and Chrbolkova, Tereza and Pacheco-Blanco, Mariana and Wang, Zhiqiang and Comer, Justin M and Zal, Tomasz and Davis, Richard Eric
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2021)

Human Lung-Resident Macrophages Colocalize with and Provide Costimulation to PD1hi Tissue-Resident Memory T Cells.
Authors: Snyder, Mark E and Sembrat, John and Noda, Kentaro and Myerburg, Michael M and Craig, Andrew and Mitash, Nilay and Harano, Takashi and Furukawa, Masashi and Pilewski, Joseph and McDyer, John and Rojas, Mauricio and Sanchez, Pablo
Journal: American journal of respiratory and critical care medicine (2021): 1230-1244

Normal Numbers of Stem Cell Memory T Cells Despite Strongly Reduced Naive T Cells Support Intact Memory T Cell Compartment in Ataxia Telangiectasia.
Authors: Weitering, Thomas J and Melsen, Janine E and van Ostaijen-Ten Dam, Monique M and Weemaes, Corry M R and Schilham, Marco W and van der Burg, Mirjam
Journal: Frontiers in immunology (2021): 686333