XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~1300 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.3 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 499 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Storage, safety and handling
H-phrase | H301, H311, H331 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R23, R24, R25 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
Overview | SDSProtocol |
Molecular weight ~1300 | Correction Factor (260 nm) 0.3 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 499 | Emission (nm) 520 | Quantum yield 0.921 |
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). XFD488 phalloidin conjugate is chemically equivalent to Alexa Fluor® 488 phalloidin. This green fluorescent phalloidin conjugate selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.
Example protocol
AT A GLANCE
Protocol Summary
- Prepare samples in microplate wells
- Remove liquid from samples in the plate
- Add XFD488 Phalloidin Conjugate solution (100 μL/well)
- Stain the cells at room temperature for 20 to 90 minutes
- Wash the cells
- Examine the specimen under microscope with FITC filter
Storage and Handling Conditions
The solution should be stable for at least 6 months if store at -20 °C. Protect the fluorescent conjugates from light, and avoid freeze/thaw cycles. Note: Phalloidin is toxic, although the amount of toxin present in a vial could be lethal only to a mosquito (LD50 of phalloidin = 2 mg/kg), it should be handled with care.PREPARATION OF WORKING SOLUTION
XFD488 Phalloidin Conjugate working solution
Add 1 µL of XFD488 Phalloidin Conjugate solution to 1 mL of PBS with 1% BSA. Note: The stock solution of phalloidin conjugate should be aliquoted and stored at -20 °C. protected from light. Note: Different cell types might be stained differently. The concentration of phalloidin conjugate working solution should be prepared accordingly.SAMPLE EXPERIMENTAL PROTOCOL
Stain the cells
- Perform formaldehyde fixation. Incubate cells with 3.0–4.0 % formaldehyde in PBS at room temperature for 10–30 minutes. Note: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.
- Rinse the fixed cells 2–3 times in PBS.
- Optional: Add 0.1% Triton X-100 in PBS into fixed cells for 3 to 5 minutes to increase permeability. Rinse the cells 2–3 times in PBS.
- Add 100 μL/well (96-well plate) of XFD488 Phalloidin Conjugate working solution into the fixed cells, and stain the cells at room temperature for 20 to 90 minutes.
- Rinse cells gently with PBS 2 to 3 times to remove excess phalloidin conjugate before plating, sealing and imaging under microscope with FITC filter set.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.3 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 499 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594* | 590 | 618 | 92000 | 0.661 | 0.43 | 0.56 |
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
Show More (2) |
Images
Figure 1. Fixed and stained HeLa cells.
HeLa cells were fixed with 4% formaldehyde, permeabilized, and blocked. F-actin were stained with XFD488 phalloidin (Cat No. 23153) and nuclei labeled with Nuclear Red™ DCS1 (Cat No. 17552). Images were acquired on a Keyence BZ-X710 all-in-one fluorescence microscope.
HeLa cells were fixed with 4% formaldehyde, permeabilized, and blocked. F-actin were stained with XFD488 phalloidin (Cat No. 23153) and nuclei labeled with Nuclear Red™ DCS1 (Cat No. 17552). Images were acquired on a Keyence BZ-X710 all-in-one fluorescence microscope.
Citations
View all 31 citations: Citation Explorer
Dicalcin suppresses in vitro trophoblast attachment in human cell lines
Authors: Saito, Ryohei and Satoh, Hiromasa and Aoba, Kayo and Hirasawa, Hajime and Miwa, Naofumi
Journal: Biochemical and Biophysical Research Communications (2021): 206--213
Authors: Saito, Ryohei and Satoh, Hiromasa and Aoba, Kayo and Hirasawa, Hajime and Miwa, Naofumi
Journal: Biochemical and Biophysical Research Communications (2021): 206--213
Atractylodin inhibits fructose-induced human podocyte hypermotility via anti-oxidant to down-regulate TRPC6/p-CaMK4 signaling
Authors: Chen, Li and Tang, Ya-Li and Liu, Zhi-Hong and Pan, Ying and Jiao, Rui-Qing and Kong, Ling-Dong
Journal: European journal of pharmacology (2021): 174616
Authors: Chen, Li and Tang, Ya-Li and Liu, Zhi-Hong and Pan, Ying and Jiao, Rui-Qing and Kong, Ling-Dong
Journal: European journal of pharmacology (2021): 174616
Targeted Gene Disruption in Pacific Oyster Based on CRISPR/Cas9 Ribonucleoprotein Complexes
Authors: Yu, Hong and Li, Huijuan and Li, Qi and Xu, Rui and Yue, Chenyang and Du, Shaojun
Journal: Marine Biotechnology (2019): 1--9
Authors: Yu, Hong and Li, Huijuan and Li, Qi and Xu, Rui and Yue, Chenyang and Du, Shaojun
Journal: Marine Biotechnology (2019): 1--9
Enhancing the cell-biological performances of hydroxyapatite bioceramic by constructing silicate-containing grain boundary phases via sol infiltration
Authors: Xu, Yubin and Lu, Teliang and He, Fupo and Ma, Ning and Ye, Ji and ong , undefined and Wu, Tingting
Journal: ACS Biomaterials Science & Engineering (2018)
Authors: Xu, Yubin and Lu, Teliang and He, Fupo and Ma, Ning and Ye, Ji and ong , undefined and Wu, Tingting
Journal: ACS Biomaterials Science & Engineering (2018)
Enhanced Osteogenesis of Injectable Calcium Phosphate Bone Cement Mediated by Loading Chondroitin Sulfate
Authors: Shi, Haishan and Ye, Xiaoling and Zhang, Jing and Ye, Ji and ong, undefined
Journal: ACS Biomaterials Science & Engineering (2018)
Authors: Shi, Haishan and Ye, Xiaoling and Zhang, Jing and Ye, Ji and ong, undefined
Journal: ACS Biomaterials Science & Engineering (2018)
Enhanced bovine serum albumin absorption on the N-hydroxysuccinimide activated graphene oxide and its corresponding cell affinity
Authors: Xiong, Kun and Fan, Qingbo and Wu, Tingting and Shi, Haishan and Chen, Lin and Yan, Minhao
Journal: Materials Science and Engineering: C (2017)
Authors: Xiong, Kun and Fan, Qingbo and Wu, Tingting and Shi, Haishan and Chen, Lin and Yan, Minhao
Journal: Materials Science and Engineering: C (2017)
Cell-Permeable, MMP-2 Activatable, Nickel Ferrite and His-tagged Fusion Protein Self-Assembled Fluorescent Nanoprobe for Tumor Magnetic Targeting and Imaging
Authors: Sun, Lu and Xie, Shuping and Qi, Jing and Liu, Ergang and Liu, Di and Liu, Quan and Chen, Sunhui and He, Huining and Yang, Victor C
Journal: ACS Applied Materials & Interfaces (2017)
Authors: Sun, Lu and Xie, Shuping and Qi, Jing and Liu, Ergang and Liu, Di and Liu, Quan and Chen, Sunhui and He, Huining and Yang, Victor C
Journal: ACS Applied Materials & Interfaces (2017)
The correlation between osteopontin adsorption and cell adhesion to mixed self-assembled monolayers of varying charges and wettability
Authors: Hao, Lijing and Li, Tianjie and Yang, Fan and Zhao, Naru and Cui, Fuzhai and Shi, Xuetao and Du, Chang and Wang, Yingjun
Journal: Biomaterials Science (2017)
Authors: Hao, Lijing and Li, Tianjie and Yang, Fan and Zhao, Naru and Cui, Fuzhai and Shi, Xuetao and Du, Chang and Wang, Yingjun
Journal: Biomaterials Science (2017)
Study on the Regulation of Focal Adesions and Cortical Actin by Matrix Nanotopography in 3D Environment
Authors: Han, Jingjing and Lin, Keng-hui and Chew, Lock Yue
Journal: Journal of Physics: Condensed Matter (2017)
Authors: Han, Jingjing and Lin, Keng-hui and Chew, Lock Yue
Journal: Journal of Physics: Condensed Matter (2017)
Enhanced osteointegration of tantalum-modified titanium implants with micro/nano-topography
Authors: Shi, Junyu and Zhang, Xiaomeng and Qiao, Shichong and Ni, Jie and Mo, Jiaji and Gu, Yingxin and Lai, Hongchang
Journal: RSC Advances (2017): 46472--46479
Authors: Shi, Junyu and Zhang, Xiaomeng and Qiao, Shichong and Ni, Jie and Mo, Jiaji and Gu, Yingxin and Lai, Hongchang
Journal: RSC Advances (2017): 46472--46479
References
View all 127 references: Citation Explorer
Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes
Authors: Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.
Journal: Urology (2011): 970 e1
Authors: Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.
Journal: Urology (2011): 970 e1
Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin
Authors: Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.
Journal: FEBS Lett (2011): 767
Authors: Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.
Journal: FEBS Lett (2011): 767
pH-(low)-insertion-peptide (pHLIP) translocation of membrane impermeable phalloidin toxin inhibits cancer cell proliferation
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Journal: Proc Natl Acad Sci U S A (2010): 20246
Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging
Authors: Chazotte B., undefined
Journal: Cold Spring Harb Protoc (2010): pdb prot4947
Authors: Chazotte B., undefined
Journal: Cold Spring Harb Protoc (2010): pdb prot4947
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Effect of Phalloidin on Filaments Polymerized from Heart Muscle Adp-Actin Monomers
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Journal: J Therm Anal Calorim (2009): 721
In vitro inhibition of OATP-mediated uptake of phalloidin using bile acid derivatives
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Journal: Toxicol Appl Pharmacol (2009): 13
Processing of the phalloidin proprotein by prolyl oligopeptidase from the mushroom Conocybe albipes
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Pygmy squids and giant brains: mapping the complex cephalopod CNS by phalloidin staining of vibratome sections and whole-mount preparations
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Authors: Wollesen T, Loesel R, Wanninger A.
Journal: J Neurosci Methods (2009): 63
Anti-acetylated tubulin antibody staining and phalloidin staining in the starlet sea anemone Nematostella vectensis
Authors: Genikhovich G, Technau U.
Journal: Cold Spring Harb Protoc (2009): pdb prot5283
Authors: Genikhovich G, Technau U.
Journal: Cold Spring Harb Protoc (2009): pdb prot5283
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
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What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?