Posted on January 22, 2020
How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader
How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader
Posted on January 22, 2020
Why is my GSH concentration greater than my Total GSH concentration? Why is my calculated GSH/TGSH ratio greater than 1?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader
Why is my GSH concentration greater than my Total GSH concentration? Why is my calculated GSH/TGSH ratio greater than 1?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader
Posted on December 10, 2019
What are the differences between traditional vs modern Gram staining?
Cell AssayColorimetricGram stainingfluorimetriclive cell assaybacteria
What are the differences between traditional vs modern Gram staining?
Cell AssayColorimetricGram stainingfluorimetriclive cell assaybacteria
Posted on August 21, 2019
Can I measure NADPH without lysing my cells?
NADH AssayNADP/NADPH ratioNADHNADPNADPHNADWestern Blotcell lysiscell assayemission wavelengthexcitation wavelengthfluorescence imagingfluorimetriclive cell analysislive cell assaymultiplex
Can I measure NADPH without lysing my cells?
NADH AssayNADP/NADPH ratioNADHNADPNADPHNADWestern Blotcell lysiscell assayemission wavelengthexcitation wavelengthfluorescence imagingfluorimetriclive cell analysislive cell assaymultiplex
Posted on July 8, 2019
What is a Western Blot?
ConjugationProteinagarose gelchemiluminescencecolorimetricfluorimetricWestern Blotprotein mass
What is a Western Blot?
ConjugationProteinagarose gelchemiluminescencecolorimetricfluorimetricWestern Blotprotein mass
Posted on June 13, 2019
Which ROS reagent is best for use in small animals such as mice or rats? What is the recommended dosage?
ROSfluorimetricin vivo imagingfluorescence imaging
Which ROS reagent is best for use in small animals such as mice or rats? What is the recommended dosage?
ROSfluorimetricin vivo imagingfluorescence imaging
Posted on June 13, 2019
What is the best way to quantitate Cyclic ADP-Ribose (cADPR)?
NADCa2+calciumcell assayfluorimetriccalcium mobilitysecond messengerfluorescent plate reader
What is the best way to quantitate Cyclic ADP-Ribose (cADPR)?
NADCa2+calciumcell assayfluorimetriccalcium mobilitysecond messengerfluorescent plate reader
Posted on June 13, 2019
Is there a multi-drug resistance assay kit that can detect both P-glycoprotein (P-gp, MDR1) and the multidrug resistance associated protein (MRP1)?
fluorimetricfluorescent plate readerMultidrug Resistance (MDR1/P-gp, MRP1)
Is there a multi-drug resistance assay kit that can detect both P-glycoprotein (P-gp, MDR1) and the multidrug resistance associated protein (MRP1)?
fluorimetricfluorescent plate readerMultidrug Resistance (MDR1/P-gp, MRP1)
Posted on May 20, 2019
Will reducing agents cause any interference when using your hydrogen peroxide assays?
ROSH2O2fluorimetrichydrogen peroxide
Will reducing agents cause any interference when using your hydrogen peroxide assays?
ROSH2O2fluorimetrichydrogen peroxide
Posted on April 2, 2019
How does the Amplite® Fluorimetric Glutathione GSH/GSSG Ratio assay work? What is the assay actually quantifying?
GSSGGSHGSH/GSSG ratiocell-permeable probesfluorimetricglutathione
How does the Amplite® Fluorimetric Glutathione GSH/GSSG Ratio assay work? What is the assay actually quantifying?
GSSGGSHGSH/GSSG ratiocell-permeable probesfluorimetricglutathione
Posted on April 2, 2019
Can Live or Dead™ Fixable Dead Cell Staining Kit (Cat# 22601) be used to stain cells after fixation?
Cell AssayOrganelle Stainingfluorimetric
Can Live or Dead™ Fixable Dead Cell Staining Kit (Cat# 22601) be used to stain cells after fixation?
Cell AssayOrganelle Stainingfluorimetric
Posted on August 15, 2018
Is there a problem with my calibration curve of GSH and Total GSH for Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit? Why does my standard curve not make sense?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader
Is there a problem with my calibration curve of GSH and Total GSH for Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit? Why does my standard curve not make sense?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader
Posted on August 15, 2018
Can I use Triton X-100 as my lysis buffer for Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence* (Cat# 10056)? Can I use X buffer to lysate/prepare my samples?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader
Can I use Triton X-100 as my lysis buffer for Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence* (Cat# 10056)? Can I use X buffer to lysate/prepare my samples?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader
Posted on August 15, 2018
How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader
How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader
Posted on August 15, 2018
Do I need to deproteinize my samples before using Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence* (Cat# 10056)?
GSHGSSGGSH/GSSG ratiofluorimetricfluorescence microplate reader
Do I need to deproteinize my samples before using Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence* (Cat# 10056)?
GSHGSSGGSH/GSSG ratiofluorimetricfluorescence microplate reader
Posted on August 10, 2018
Why is the Total GSH standard solution concentration equal to twice that of the GSSG standard solution concentration? Why is [GSSG] equal to ([Total GSH] - [GSH]) DIVIDED by 2?
GSHGSSGglutathioneGSH/GSSG ratiofluorimetricfluorescent plate reader
Why is the Total GSH standard solution concentration equal to twice that of the GSSG standard solution concentration? Why is [GSSG] equal to ([Total GSH] - [GSH]) DIVIDED by 2?
GSHGSSGglutathioneGSH/GSSG ratiofluorimetricfluorescent plate reader
Posted on July 8, 2018
The probe provided in your GSH/GSSG ratio assay (Cat#10060) only reacts with reduced GSH, not GSSG. Why do I also need to prepare a GSSG standard curve?
GSHGSSGfluorimetric
The probe provided in your GSH/GSSG ratio assay (Cat#10060) only reacts with reduced GSH, not GSSG. Why do I also need to prepare a GSSG standard curve?
GSHGSSGfluorimetric
Posted on June 28, 2018
Which hydrogen peroxide assays can I use if NADH is present in my sample?
ROSH2O2fluorimetric
Which hydrogen peroxide assays can I use if NADH is present in my sample?
ROSH2O2fluorimetric