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AAT Bioquest

Posted on January 22, 2024
What are the structures of protein?
Antibodies and Proteomics Peptide and Oligonucleotide LabelingProtein Assays & Analysis


Posted on January 22, 2024
What are the similarities between protein A and protein G?
Antibodies and Proteomics Peptide and Oligonucleotide LabelingProtein Assays & Analysis


Posted on August 29, 2023
What are molecular beacons used for?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on August 29, 2023
How does a molecular beacon work?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What is the importance of molecular beacons?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the advantages of molecular beacon probes?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the limitations of molecular beacon probes?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
Why are MBs particularly suited for mRNA studies?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the strategies for minimizing MB false-positive signals in living cells?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the properties of molecular beacons that affect nucleic acid detection?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the widely used detection methods in quantitative PCR (Q-PCR)
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the steps of a molecular beacon RT-PCR assay?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the best ways to store a molecular beacon preparation?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on August 29, 2023
What are the reasons for the low signal-to-background ratio after the synthesis and purification of a molecular beacon and how do I solve the problem?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)PurificationReal-Time PCR (qPCR)


Posted on June 2, 2022
What is the difference between Tide Quencher 2 (TQ2) and Tide Quencher 2WS (TQ2WS) products?
Tide Quencher Dyes Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingAntibody and Protein Labeling Chemical Reagents


Posted on November 14, 2020
How do you purify oligonucleotide conjugates by HPLC?
Bioconjugation Chemical Reagents Peptide and Oligonucleotide LabelingConjugationPeptide and Oligonucleotide


Posted on October 19, 2020
Why does my DNA oligonucleotide have mutations?
Peptide and Oligonucleotide LabelingDNADNA and RNAPeptide and Oligonucleotide


Posted on October 19, 2020
How long can I store the oligonucleotide?
Peptide and Oligonucleotide LabelingPeptide and OligonucleotideStorage


Posted on June 22, 2020
Are there different types of fluorescent probes used in qPCR?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationDNA and RNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on May 9, 2020
What are reverse transcription primers?
Antibodies and Proteomics Peptide and Oligonucleotide LabelingCellular ProcessesDNA and RNADNA and RNA QuantitationPeptide and OligonucleotideRNAPCR primer


Posted on May 9, 2020
What are oligonucleotides?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNAFRETPeptide and Oligonucleotidefluorescence imaging


Posted on April 24, 2020
Is there a difference between tagging GFP at the N-terminus and at the C-terminus of a gene?
Peptide and Oligonucleotide LabelingPeptide and OligonucleotideProtein


Posted on April 24, 2020
What is the difference between ethidium homodimer and dihydroethidium in terms of staining?
Peptide and Oligonucleotide LabelingCell ViabilityDNA and RNA QuantitationDNA and RNAFluorescenceNucleic AcidsPhysiological Probes


Posted on April 7, 2020
What rhodamine dye do you have for automated DNA sequencing and nucleotide labeling?
Chemical Reagents Peptide and Oligonucleotide LabelingClassic DyesDNA sequencingFluoresceinsRhodamines and Rhodamine DerivativesPhysiological Probes


Posted on April 7, 2020
6-VIC, SE is a popular amino-reactive fluorescent probe that is widely used in nucleic acid sequencing and related research. Is it also often used for labeling oligos?
Peptide and Oligonucleotide LabelingDNA sequencingPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on April 7, 2020
Share several ways of labeling an oligonucleotide with fluorescein like 6-FAM/6-HEX/6-TET phosphoramidite?
Chemical Reagents Peptide and Oligonucleotide LabelingClassic DyesFluoresceinsPhysiological Probes