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Chemical Building Blocks
AAT Bioquest provides a comprehensive selection of chemical building blocks for synthesizing fluorescent probes, labeled oligonucleotides, FRET-based peptide substrates, and bioconjugates. Our portfolio includes click chemistry reagents, crosslinkers, fluorescent dye precursors, and specialty building blocks enabling researchers to construct custom probes for applications ranging from qPCR and enzyme assays to protein labeling and live cell imaging.
Category | Primary Use | Key Products |
|---|---|---|
Fluorescent Dye Precursors | Dye synthesis | Resorcinols, 3,6-Dichlorotrimellitic anhydride |
Click Chemistry Building Blocks | Bioorthogonal labeling, site-specific conjugation | Azides, Alkynes, DBCO, BCN, TCO, Tetrazines |
Crosslinkers | Protein conjugation, antibody-enzyme coupling | NHS/Maleimide, Buccutite™, Photoaffinity |
Peptide Building Blocks | FRET substrates, protease assays | FMOC-Lys/Asp/Glu(Dye), Tide Quencher™ acids/amines |
Oligonucleotide Building Blocks | Labeled probes, qPCR, molecular beacons | CPG reagents, Phosphoramidites, FRET dyes |
Fluorescent Dye Precursors
Precursors chemicals are essential for synthesizing fluorescent dyes used in qPCR, enzyme assays, and fluorescence imaging. Fluororesorcinol derivatives serve as starting materials for spectrally-shifted fluorescein, coumarin, and resorufin analogs, while 3,6-dichlorotrimellitic anhydride is the key intermediate for TET, HEX, and JOE — widely used dichlorinated fluorescein reporter dyes in qPCR multiplexing applications.
Click Chemistry Building Blocks
Click Chemistry Building Blocks provide functionalized fluorescent dyes and reactive intermediates for both copper-catalyzed (CuAAC) and copper-free labeling reactions.
- Alkyne building blocks for CuAAC reactions with azide-labeled biomolecules
- DBCO reagents enabling copper-free click chemistry with azides
- BCN compounds with smaller steric profiles for strain-promoted reactions
- TCO reagents for ultrafast inverse electron-demand Diels-Alder reactions
- Tetrazine dyes compatible with TCO and cyclopropene groups
- Azide-functionalized dyes for alkyne-labeled targets
Browse by Type: Alkyne Building Blocks | DBCO Building Blocks | BCN Building Blocks | TCO Building Blocks | Tetrazine Building Blocks | Azide Building Blocks
Crosslinkers
Crosslinkers enable protein-protein coupling and antibody-enzyme conjugation with higher yields and minimal loss of binding activity.
- Buccutite™ MTA-FOL coupling chemistry for efficient protein conjugation
- Heterobifunctional NHS/maleimide crosslinkers for two-step amine-thiol conjugation
- Click chemistry crosslinkers using copper-free DBCO-azide reactions
- Photoaffinity crosslinkers with UV-activated diazirine, benzophenone, and azide probes
- Psoralen DNA crosslinkers for nucleic acid applications
- Multiple spacer arm lengths available (8.3Å to 24.6Å)
Peptide Building Blocks
Peptide Building Blocks provide pre-labeled amino acids and specialty reagents compatible with standard Fmoc solid-phase peptide synthesis.
- FMOC-Lys(Dye) for side-chain labeling with fluorescent dyes and quenchers
- FMOC-Asp(Dye) for carboxylic acid side-chain labeling, useful for N-terminal positioning
- FMOC-Glu(Dye) providing longer side-chain spacer than aspartate
- Special amino acids including statine derivatives and monoprotected diamines
- Tide Quencher™ acids and amines optimized for FRET peptide substrates
Oligonucleotide Building Blocks
Oligonucleotide Building Blocks provide specialized reagents for oligonucleotide synthesis and modification using standard phosphoramidite chemistry.
- CPG reagents (controlled pore glass) for 3' labeling with dyes, quenchers, biotin, and amines
- Phosphoramidites for 5'-end and internal labeling of oligonucleotides
- Tide Fluor™ photostable fluorophores optimized for FRET and qPCR
- Tide Quencher™ (TQ1-TQ5) dark quenchers with minimal background
- Click chemistry modifiers for bioorthogonal post-synthetic labeling
Browse by Type: CPG Reagents | Phosphoramidites
This document (01.0350.260113r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.