PE-iFluor® 700 Tandem
PE-iFluor® 700 Tandem is a new color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~720 nm. It has been validated with a spectral flow cytometer. PE-iFluor® 700 Tandem gives a significantly improved staining index than the corresponding PE-Alexa Fluor™ 700 Tandem. AAT Bioquest offer the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.
![Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 700 specific B10-A channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fpe-ifluor-700-tandem%2Ffigure-for-pe-ifluor-700-tandem_A5bhN.png&w=640&q=75)
![Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 700 specific B10-A channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fpe-ifluor-700-tandem%2Ffigure-for-pe-ifluor-700-tandem_A5bhN.png&w=640&q=75)
![Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 700 specific B10-A channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fpe-ifluor-700-tandem%2Ffigure-for-pe-ifluor-700-tandem_A5bhN.png&w=128&q=25)
![Flow cytometry analysis of whole blood stained with PE-iFluor® 700 anti-human CD19 *HIB19* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 700 specific B10-A channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fpe-ifluor-700-tandem%2Ffigure-for-pe-ifluor-700-tandem_cs7PK.png&w=128&q=25)
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
PE-iFluor® 610 Tandem | 565 | 625 | 1960000 |
PE-iFluor® 647 Tandem | 565 | 666 | 1960000 |
PE-iFluor® 750 Tandem | 565 | 778 | 1960000 |
PE-iFluor® 710 Tandem | 565 | 747 | 1960000 |
PE-iFluor® 594 Tandem | 565 | 606 | 1960000 |
PE-iFluor® 660 Tandem | 565 | 695 | 1960000 |
PE-iFluor® 780 Tandem | 566 | 575 | 1960000 |
PE-iFluor® 597 Tandem | 565 | 612 | - |
PE-iFluor® 740 Tandem | 565 | 767 | 1960000 |
Show More (3) |
References
View all 13 references: Citation Explorer
Multifunctional Silica-Based Nanoparticles with Controlled Release of Organotin Metallodrug for Targeted Theranosis of Breast Cancer.
Authors: Ovejero Paredes, Karina and Díaz-García, Diana and García-Almodóvar, Victoria and Lozano Chamizo, Laura and Marciello, Marzia and Díaz-Sánchez, Miguel and Prashar, Sanjiv and Gómez-Ruiz, Santiago and Filice, Marco
Journal: Cancers (2020)
Authors: Ovejero Paredes, Karina and Díaz-García, Diana and García-Almodóvar, Victoria and Lozano Chamizo, Laura and Marciello, Marzia and Díaz-Sánchez, Miguel and Prashar, Sanjiv and Gómez-Ruiz, Santiago and Filice, Marco
Journal: Cancers (2020)
Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line.
Authors: Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar
Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29
Authors: Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar
Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29
[Changes of monocyte and monocyte-platelet aggregates in different subgroups of thrombotic events in patients with acute myocardial infarction during PCI].
Authors: Wang, Sheng and Sun, Cuifang and Liao, Wang and Wu, Zhongwei and Wang, Yudai and Huang, Xiuxian and Lu, Sijia and Dong, Xiaoli and Shuai, Fujie and Li, Bin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2017): 959-965
Authors: Wang, Sheng and Sun, Cuifang and Liao, Wang and Wu, Zhongwei and Wang, Yudai and Huang, Xiuxian and Lu, Sijia and Dong, Xiaoli and Shuai, Fujie and Li, Bin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2017): 959-965
Novel PSCA targeting scFv-fusion proteins for diagnosis and immunotherapy of prostate cancer.
Authors: Kessler, Claudia and Pardo, Alessa and Tur, Mehmet K and Gattenlöhner, Stefan and Fischer, Rainer and Kolberg, Katharina and Barth, Stefan
Journal: Journal of cancer research and clinical oncology (2017): 2025-2038
Authors: Kessler, Claudia and Pardo, Alessa and Tur, Mehmet K and Gattenlöhner, Stefan and Fischer, Rainer and Kolberg, Katharina and Barth, Stefan
Journal: Journal of cancer research and clinical oncology (2017): 2025-2038
Combination of CD157 and FLAER to Detect Peripheral Blood Eosinophils by Multiparameter Flow Cytometry.
Authors: Carulli, Giovanni and Marini, Alessandra and Sammuri, Paola and Domenichini, Cristiana and Ottaviano, Virginia and Pacini, Simone and Petrini, Mario
Journal: Journal of clinical and experimental hematopathology : JCEH (2015): 55-60
Authors: Carulli, Giovanni and Marini, Alessandra and Sammuri, Paola and Domenichini, Cristiana and Ottaviano, Virginia and Pacini, Simone and Petrini, Mario
Journal: Journal of clinical and experimental hematopathology : JCEH (2015): 55-60