Wheat Germ Agglutinin, XFD488 Labeled XFD488 Same Structure to Alexa Fluor™ 488

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Spectral properties

Correction Factor (260 nm)	0.30
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Correction Factor (260 nm)

0.30

Correction Factor (280 nm)

0.11

Extinction coefficient (cm ^-1 M ^-1)

71000

Excitation (nm)

499

Emission (nm)

520

Quantum yield

0.92¹

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). Wheat germ agglutinin (WGA) is a lectin that binds to N-acetyl-D-glucosamine and sialic acid. It is of the most studied and useful lectins for its biological applications. Since WGA binds to glycoconjugates its derivatives and conjugates are widely used to label cell membranes and fibrotic scar tissue for fluorescence imaging and analysis. The carbohydrate-binding specificity of WGA is directed against sequences of β-1,4-GlcNAc-linked residues, the chitodextrins. Each monomer contains two identical, non-interacting binding sites, which are complementary to 3 or 4 β-1,4-GlcNAc units. Of the monosaccharides examined, only GlcNAc binds to WGA. ManNAc does not bind, and GalNAc binds only weakly. XFD488 conjugate of WGA is equivalent to the Alexa Fluor® 488 conjugate of WGA. It exhibits bright, green fluorescence. XFD488 WGA conjugate binds to sialic acid and N-acetylglucosaminyl residues.

Platform

Fluorescence microscope

Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.30
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Wheat Germ Agglutinin, XFD594 Labeled XFD594 Same Structure to Alexa Fluor™ 594	590	618	90000	0.66¹	0.43	0.56
Wheat Germ Agglutinin, XFD350 Labeled XFD350 Same Structure to Alexa Fluor® 350	343	441	19000	-	0.25	0.19
Wheat Germ Agglutinin, XFD532 Labeled XFD532 Same Structure to Alexa Fluor® 532	534	553	81000	0.61¹	0.24	0.09
Wheat Germ Agglutinin, XFD555 Labeled XFD555 Same Structure to Alexa Fluor® 555	553	568	150000	0.1¹	0.08	0.08
Wheat Germ Agglutinin, XFD647 Labeled XFD647 Same Structure to Alexa Fluor® 647	650	671	239000	0.33¹	0.00	0.03
Wheat Germ Agglutinin, XFD750 Labeled XFD750 Same Structure to Alexa Fluor® 750	752	776	240000	0.12¹	0.00	0.04

Images

Figure 1. Live HeLa cells were stained with Wheat Germ Agglutinin, XFD488 labeled at 5 µg/mL for 30 minutes followed by Hoechst 33342 (Cat No. 17535). Image was acquired using fluorescence microscopy using FITC and DAPI filter set.

References

View all 50 references: Citation Explorer

Wheat germ agglutinin is involved in the protective action of 24-epibrassinolide on the roots of wheat seedlings under drought conditions.
Authors: Avalbaev, Azamat and Bezrukova, Marina and Allagulova, Chulpan and Lubyanova, Alsu and Kudoyarova, Guzel and Fedorova, Kristina and Maslennikova, Dilara and Yuldashev, Ruslan and Shakirova, Farida
Journal: Plant physiology and biochemistry : PPB (2020): 420-427

Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
Authors: Janković, Tamara and Goč, Sanja and Mitić, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava
Journal: Upsala journal of medical sciences (2020): 10-18

Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.
Authors: Guevara, Rebekah B and Fox, Barbara A and Bzik, David J
Journal: mSphere (2020)

Wheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.
Authors: Killilea, David W and McQueen, Rebecca and Abegania, Judi R
Journal: Journal of food science (2020): 808-815

Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.
Authors: Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu
Journal: Colloids and surfaces. B, Biointerfaces (2020): 110572

Wheat germ agglutinin-conjugated fluorescent pH sensors for visualizing proton fluxes.
Authors: Zhang, Lejie and Zhang, Mei and Bellve, Karl and Fogarty, Kevin E and Castro, Maite A and Brauchi, Sebastian and Kobertz, William R
Journal: The Journal of general physiology (2020)

Bacterial expression, purification and biophysical characterization of wheat germ agglutinin and its four hevein-like domains.
Authors: Leyva, Eduardo and Medrano-Cerano, Jorge L and Cano-Sánchez, Patricia and López-González, Itzel and Gómez-Velasco, Homero and Del Río-Portilla, Federico and García-Hernández, Enrique
Journal: Biopolymers (2019): e23242

Targeted delivery of etoposide, carmustine and doxorubicin to human glioblastoma cells using methoxy poly(ethylene glycol)‑poly(ε‑caprolactone) nanoparticles conjugated with wheat germ agglutinin and folic acid.
Authors: Kuo, Yung-Chih and Chang, Yu-Hsuan and Rajesh, Rajendiran
Journal: Materials science & engineering. C, Materials for biological applications (2019): 114-128

Wheat Germ Agglutinin as a Potential Therapeutic Agent for Leukemia.
Authors: Ryva, Bradley and Zhang, Keman and Asthana, Abhishek and Wong, Derek and Vicioso, Yorleny and Parameswaran, Reshmi
Journal: Frontiers in oncology (2019): 100

Enhanced anti-colon cancer efficacy of 5-fluorouracil by epigallocatechin-3- gallate co-loaded in wheat germ agglutinin-conjugated nanoparticles.
Authors: Wang, Ruoning and Huang, Jinyu and Chen, Jian and Yang, Mengmeng and Wang, Honglan and Qiao, Hongzhi and Chen, Zhipeng and Hu, Lihong and Di, Liuqing and Li, Junsong
Journal: Nanomedicine : nanotechnology, biology, and medicine (2019): 102068