Wheat Germ Agglutinin, XFD555 Labeled *XFD555 Same Structure to Alexa Fluor® 555*
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
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International | See distributors |
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Shipping | Standard overnight for United States, inquire for international |
Physical properties
Solvent | Water |
Spectral properties
Correction Factor (260 nm) | 0.08 |
Correction Factor (280 nm) | 0.08 |
Extinction coefficient (cm -1 M -1) | 150000 |
Excitation (nm) | 553 |
Emission (nm) | 568 |
Quantum yield | 0.11 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
Correction Factor (260 nm) 0.08 | Correction Factor (280 nm) 0.08 | Extinction coefficient (cm -1 M -1) 150000 | Excitation (nm) 553 | Emission (nm) 568 | Quantum yield 0.11 |
XFD555, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 555, a trademark of ThermoFisher. Wheat germ agglutinin (WGA) is a well-researched lectin known for its valuable biological applications. Due to its ability to bind to glycoconjugates, WGA derivatives, and conjugates are widely used to label yeast bud scars, fibrotic scar tissue, and the cell membrane of gram bacteria and mammalian cells. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer possesses two identical, non-interacting binding sites that complement 3 or 4 β-1,4-GlcNAc units. Among the tested monosaccharides, only GlcNAc exhibits binding to WGA, while ManNAc does not bind, and GalNAc demonstrates weak binding. XFD555 WGA conjugate, like its counterpart Alexa Fluor® 555 WGA conjugate, emits a bright orange fluorescence and is useful in a variety of applications, including immunofluorescence (IF), immunohistochemistry (IHC), and flow cytometry (FC).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.08 |
Correction Factor (280 nm) | 0.08 |
Extinction coefficient (cm -1 M -1) | 150000 |
Excitation (nm) | 553 |
Emission (nm) | 568 |
Quantum yield | 0.11 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Wheat Germ Agglutinin, XFD488 Labeled *XFD488 Same Structure to Alexa Fluor™ 488* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
Wheat Germ Agglutinin, XFD594 Labeled *XFD594 Same Structure to Alexa Fluor™ 594* | 590 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
Wheat Germ Agglutinin, XFD350 Labeled *XFD350 Same Structure to Alexa Fluor® 350* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
Wheat Germ Agglutinin, XFD532 Labeled *XFD532 Same Structure to Alexa Fluor® 532* | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
Wheat Germ Agglutinin, XFD647 Labeled *XFD647 Same Structure to Alexa Fluor® 647* | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
Wheat Germ Agglutinin, XFD750 Labeled *XFD750 Same Structure to Alexa Fluor® 750* | 752 | 776 | 240000 | 0.121 | 0.00 | 0.04 |
References
View all 50 references: Citation Explorer
A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part III: Reagents for Actin, Tubulin, Cellular Membranes, and Whole Cell and Cytoplasm.
Authors: Golub, Oggie and Kilgore, Jason A and Dolman, Nick J
Journal: Current protocols (2023): e754
Authors: Golub, Oggie and Kilgore, Jason A and Dolman, Nick J
Journal: Current protocols (2023): e754
Evaluation of human platelet granules by structured illumination laser fluorescence microscopy.
Authors: Pluthero, Fred G and Kahr, Walter H A
Journal: Platelets (2023): 2157808
Authors: Pluthero, Fred G and Kahr, Walter H A
Journal: Platelets (2023): 2157808
Vascular Endothelial Glycocalyx Plays a Role in the Obesity Paradox According to Intravital Observation.
Authors: Mitsuda, Shingo and Uzawa, Kohji and Sawa, Marie and Ando, Tadao and Yoshikawa, Takahiro and Miyao, Hideki and Yorozu, Tomoko and Ushiyama, Akira
Journal: Frontiers in cardiovascular medicine (2021): 727888
Authors: Mitsuda, Shingo and Uzawa, Kohji and Sawa, Marie and Ando, Tadao and Yoshikawa, Takahiro and Miyao, Hideki and Yorozu, Tomoko and Ushiyama, Akira
Journal: Frontiers in cardiovascular medicine (2021): 727888
Loss of troponin-T labelling in endomyocardial biopsies of cardiac transplant patients is associated with increased rejection grading.
Authors: Isaacson, Mikaiah and Gibbs, Helen and Ruygrok, Peter N and Crossman, David J
Journal: Clinical and experimental pharmacology & physiology (2020)
Authors: Isaacson, Mikaiah and Gibbs, Helen and Ruygrok, Peter N and Crossman, David J
Journal: Clinical and experimental pharmacology & physiology (2020)
Fluorescence background quenching as a means to increase Signal to Background ratio - a proof of concept during Nerve Imaging.
Authors: Buckle, Tessa and van der Wal, Steffen and van Willigen, Danny M and Aalderink, Germaine and KleinJan, Gijs H and van Leeuwen, Fijs W B
Journal: Theranostics (2020): 9890-9898
Authors: Buckle, Tessa and van der Wal, Steffen and van Willigen, Danny M and Aalderink, Germaine and KleinJan, Gijs H and van Leeuwen, Fijs W B
Journal: Theranostics (2020): 9890-9898
3D-electron microscopic characterization of interstitial cells in the human bladder upper lamina propria.
Authors: Neuhaus, Jochen and Schröppel, Birgit and Dass, Martin and Zimmermann, Hans and Wolburg, Hartwig and Fallier-Becker, Petra and Gevaert, Thomas and Burkhardt, Claus J and Do, Hoang Minh and Stolzenburg, Jens-Uwe
Journal: Neurourology and urodynamics (2018): 89-98
Authors: Neuhaus, Jochen and Schröppel, Birgit and Dass, Martin and Zimmermann, Hans and Wolburg, Hartwig and Fallier-Becker, Petra and Gevaert, Thomas and Burkhardt, Claus J and Do, Hoang Minh and Stolzenburg, Jens-Uwe
Journal: Neurourology and urodynamics (2018): 89-98
Highly specific detection of muscarinic M3 receptor, G protein interaction and intracellular trafficking in human detrusor using Proximity Ligation Assay (PLA).
Authors: Berndt-Paetz, Mandy and Herbst, Luise and Weimann, Annett and Gonsior, Andreas and Stolzenburg, Jens-Uwe and Neuhaus, Jochen
Journal: Acta histochemica (2018): 329-339
Authors: Berndt-Paetz, Mandy and Herbst, Luise and Weimann, Annett and Gonsior, Andreas and Stolzenburg, Jens-Uwe and Neuhaus, Jochen
Journal: Acta histochemica (2018): 329-339
Chitin distribution in the Oithona digestive and reproductive systems revealed by fluorescence microscopy.
Authors: Sugier, Kevin and Vacherie, Benoit and Cornils, Astrid and Wincker, Patrick and Jamet, Jean-Louis and Madoui, Mohammed-Amin
Journal: PeerJ (2018): e4685
Authors: Sugier, Kevin and Vacherie, Benoit and Cornils, Astrid and Wincker, Patrick and Jamet, Jean-Louis and Madoui, Mohammed-Amin
Journal: PeerJ (2018): e4685
Classification of Cells in CTC-Enriched Samples by Advanced Image Analysis.
Authors: de Wit, Sanne and Zeune, Leonie L and Hiltermann, T Jeroen N and Groen, Harry J M and Dalum, Guus van and Terstappen, Leon W M M
Journal: Cancers (2018)
Authors: de Wit, Sanne and Zeune, Leonie L and Hiltermann, T Jeroen N and Groen, Harry J M and Dalum, Guus van and Terstappen, Leon W M M
Journal: Cancers (2018)
WGA-Alexa Conjugates for Axonal Tracing.
Authors: Levy, Sabrina L and White, Joshua J and Lackey, Elizabeth P and Schwartz, Lindsey and Sillitoe, Roy V
Journal: Current protocols in neuroscience (2017): 1.28.1-1.28.24
Authors: Levy, Sabrina L and White, Joshua J and Lackey, Elizabeth P and Schwartz, Lindsey and Sillitoe, Roy V
Journal: Current protocols in neuroscience (2017): 1.28.1-1.28.24
Application notes
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