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AAT Bioquest

Posted on September 28, 2021
What does cross adsorbed secondary antibody mean?
Secondary AntibodiesAntibodies and ProteomicsPrimary AntibodiesIndocyanine Green


Posted on September 28, 2021
How long do primary antibodies last?
Primary AntibodiesAntibodies and Proteomics


Posted on September 28, 2021
Are primary antibodies protein specific?
Antibodies and ProteomicsPrimary Antibodies


Posted on September 28, 2021
What is the role of secondary antibodies?
Secondary AntibodiesPrimary AntibodiesImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)


Posted on September 24, 2021
What IgG class or subclass should I use for my primary antibody?
Protein to Protein ConjugationSecondary AntibodiesPrimary AntibodiesAntibody and Protein Labeling


Posted on September 23, 2021
What are the major benefits of using a secondary antibody
Antibodies and ProteomicsPrimary AntibodiesSecondary Antibodies


Posted on September 22, 2021
What are the major differences between primary and secondary antibodies?
Secondary AntibodiesPrimary AntibodiesAntibody and Protein Labeling


Posted on February 10, 2021
What are the different types of stem cells?
Primary AntibodiesiFluor Dyes and KitsPhysiological Probes


Posted on June 1, 2020
What is the difference between direct and sandwich ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDirect ELISAPrimary Antibodies


Posted on June 1, 2020
What is the difference between direct and indirect ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDirect ELISAPrimary AntibodiesSecondary Antibodies


Posted on April 24, 2020
Can I use unstained cells as negative controls for FACS analysis?
Antibodies and Proteomics BioconjugationPrimary Antibodiesantibody conjugation


Posted on April 24, 2020
Why does a secondary antibody have to originate from a host species different than that of the primary antibody?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies


Posted on April 24, 2020
What are the criteria for choosing primary and secondary antibodies in western blotting?
Antibodies and ProteomicsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 24, 2020
Can I use an HRP-conjugated primary antibody for western blot (with no secondary)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingHRPHRP-labeled primary antibodyHorseradish Peroxidase (HRP) and Poly-HRPPrimary AntibodiesSecondary AntibodiesWestern Blotantibody conjugation


Posted on April 24, 2020
What is the difference between indirect and direct imaging?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodiesimmunofluorescence


Posted on April 17, 2020
In IHC what should be incubation time for primary antibody and secondary antibody ?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies


Posted on April 17, 2020
Can we co-incubate the primary and secondary antibodies at the same time for Western Blotting ?
Antibodies and ProteomicsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 17, 2020
Does adding triton with the primary antibody hinder antigen detection in immunohistochemistry?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary Antibodies Chemical ReagentsBuffer Preparations and RecipesIHCbufferdetergent