FAQ

Posted on September 13, 2024
What factors should I consider when performing fluorescent western blots?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on September 13, 2024
How do I optimize chemiluminescent western blots?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingPrimary AntibodiesSecondary Antibodieschemiluminescence

Posted on September 13, 2024
What are the possible causes and solutions for background issues (high, uneven, or speckled)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingPrimary AntibodiesSecondary Antibodiesdetergent

Posted on September 13, 2024
What are the possible causes for nonspecific or diffuse bands for my western blot experiment?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on July 16, 2024
How do I choose the right secondary antibody?
Antibodies and ProteomicsCellular Structures and OrganellesPrimary AntibodiesSecondary Antibodies

Posted on May 8, 2024
Can I use a conjugated primary antibody in my immunofluorescence experiment?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingPrimary AntibodiesSecondary Antibodiesimmunofluorescence

Posted on May 8, 2024
What are the applications of 3,3'-Diaminobenzidine (DAB)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies

Posted on May 8, 2024
What are the common causes of background staining in immunohistochemistry?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies

Posted on May 8, 2024
How to reduce the background staining in immunohistochemistry?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies

Posted on April 10, 2024
When should I use fluorescence detection methods for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 10, 2024
What are the disadvantages of fluorescence detection method for western blot?
Antibodies and Proteomics BioconjugationFluorescencePrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 10, 2024
What are the advantages of fluorescence detection methods for western blot?
Antibodies and Proteomics BioconjugationFluorescencePrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 10, 2024
When should I use a chemiluminescent detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 10, 2024
What are the disadvantages of Chemiluminescent detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 10, 2024
What are the advantages of Chemiluminescent detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 10, 2024
When should I use colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 10, 2024
What are the disadvantages of colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 10, 2024
What are the advantages of colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 10, 2024
What are the most commonly used enzymes in colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 10, 2024
How do I choose the right western blot detection method?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 10, 2024
What are the disadvantages of the radioactive detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 10, 2024
What should I do if I have a high background (uniform distribution) in western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence

Posted on April 10, 2024
What should I do to get optimal transfer results?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary Antibodiesimmunofluorescence

Posted on April 10, 2024
What roles do SDS and alcohol play in the western blot transfer step?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence

Posted on April 10, 2024
What are the different types of transfer methods in western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence

Posted on April 10, 2024
How do I validate the transfer?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary Antibodiesimmunofluorescence

Posted on April 10, 2024
Why is the data produced with a western blot typically considered to be semi-quantitative?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence

Posted on April 10, 2024
What are the steps involved in southern blotting?
DNA and RNA QuantitationEnzymesGel ElectrophoresisPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 10, 2024
What are the principles of southern blotting?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence

Posted on April 10, 2024
What are the principles of western blotting?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence

Posted on March 11, 2024
Why do we need controls for immunofluorescence?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingPrimary AntibodiesSecondary Antibodiesimmunofluorescence

Posted on March 11, 2024
What do the different types of controls for immunofluorescence do?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingControlsPrimary AntibodiesSecondary Antibodiesimmunofluorescence

Posted on September 28, 2021
What does cross adsorbed secondary antibody mean?
Secondary AntibodiesAntibodies and ProteomicsPrimary AntibodiesIndocyanine Green

Posted on September 28, 2021
How long do primary antibodies last?
Primary AntibodiesAntibodies and Proteomics

Posted on September 28, 2021
Are primary antibodies protein specific?
Antibodies and ProteomicsPrimary Antibodies

Posted on September 28, 2021
What is the role of secondary antibodies?
Secondary AntibodiesPrimary AntibodiesImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)

Posted on September 24, 2021
What IgG class or subclass should I use for my primary antibody?
Protein to Protein ConjugationSecondary AntibodiesPrimary AntibodiesAntibody and Protein Labeling

Posted on September 23, 2021
What are the major benefits of using a secondary antibody
Antibodies and ProteomicsPrimary AntibodiesSecondary Antibodies

Posted on September 22, 2021
What are the major differences between primary and secondary antibodies?
Secondary AntibodiesPrimary AntibodiesAntibody and Protein Labeling

Posted on February 10, 2021
What are the different types of stem cells?
Primary AntibodiesiFluor Dyes and KitsPhysiological Probes

Posted on June 1, 2020
What is the difference between direct and sandwich ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDirect ELISAPrimary Antibodies

Posted on June 1, 2020
What is the difference between direct and indirect ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDirect ELISAPrimary AntibodiesSecondary Antibodies

Posted on April 24, 2020
Can I use unstained cells as negative controls for FACS analysis?
Antibodies and Proteomics BioconjugationPrimary Antibodiesantibody conjugation

Posted on April 24, 2020
Why does a secondary antibody have to originate from a host species different than that of the primary antibody?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies

Posted on April 24, 2020
What are the criteria for choosing primary and secondary antibodies in western blotting?
Antibodies and ProteomicsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 24, 2020
Can I use an HRP-conjugated primary antibody for western blot (with no secondary)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingHRPHRP-labeled primary antibodyHorseradish Peroxidase (HRP) and Poly-HRPPrimary AntibodiesSecondary AntibodiesWestern Blotantibody conjugation

Posted on April 24, 2020
What is the difference between indirect and direct imaging?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodiesimmunofluorescence

Posted on April 17, 2020
In IHC what should be incubation time for primary antibody and secondary antibody ?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies

Posted on April 17, 2020
Can we co-incubate the primary and secondary antibodies at the same time for Western Blotting ?
Antibodies and ProteomicsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 17, 2020
Does adding triton with the primary antibody hinder antigen detection in immunohistochemistry?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary Antibodies Chemical ReagentsBuffer Preparations and RecipesIHCbufferdetergent