Name: iFluor® 555 goat anti-mouse IgG (H+L)
Brand: AAT Bioquest
SKU: 16460
Availability: InStock

iFluor® 555 goat anti-mouse IgG (H+L)

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 555 goat anti-mouse IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~559 nm and ~569 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 555 goat anti-mouse IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).

HeLa cells were incubated with mouse anti-tubulin followed by AAT’s iFluor<sup>TM</sup> 555 goat anti-mouse IgG conjugate (Red, Right) or goat anti-mouse IgG conjugated with Alexa Fluor<sup>®</sup> 555  (Red, left), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).

Protocol

Catalog	Size	Price	Quantity
16460	200 ug	Price
16739	1 mg	Price

Citations

View all 8 citations: Citation Explorer

Specific induction of right ventricular-like cardiomyocytes from human pluripotent stem cells

Authors:

Saito, Yukihiro and Nakamura, Kazufumi and Katanosaka, Yuki and Iida, Toshihiro and Kusumoto, Dai and Sato, Ryushi and Adachi, Riki and Shimizu, Satoshi and Kurokawa, Junko and Akagi, Satoshi and others,

Journal:

Stem Cell Research \& Therapy (2025): 519

Curcumin inhibits the pruritus in mice through mast cell MrgprB2 receptor

Authors:

Jiang, Yucui and Zong, Yingxin and Du, Ying and Zhang, Miaomiao and Ye, Fan and Zhang, Jian and Yang, Yan and Zhu, Chan and Tang, Zongxiang

Journal:

Inflammation Research (2023): 1--13

The roles and molecular mechanisms of long non-coding RNA WT1-AS in the maintenance and development of gastric cancer stem cells

Authors:

Zhang, Xiaobei and Jin, Meng and Liu, Shiqi and Zang, Mingde and Hu, Lei and Du, Tao and Zhang, Baogui

Journal:

Heliyon (2023)

{\.I}nd{\"u}klenmi{\c{s}} pluripotent k{\"o}k h{\"u}cre tabanl{\i} multiple skleroz modelinde oligodendrositlere {\"o}zg{\"u} fenotiplerin ara{\c{s}}t{\i}r{\i}lmas{\i}

Authors:

Kurt Vatanda{\c{s}}lar, Burcu

Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.

Authors:

Zhao, Jingkun and Ou, Baochi and Feng, Hao and Wang, Puxiongzhi and Yin, Shuai and Zhu, Congcong and Wang, Shenjie and Chen, Chun and Zheng, Minhua and Zong, Yaping and others, undefined

Journal:

Oncotarget (2017)

References

View all 191 references: Citation Explorer

Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding

Authors:

Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.

Journal:

Med Clin (Barc) (2011): 668

Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers

Authors:

Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.

Journal:

Oncotarget (2011): 728

Falsely elevated tacrolimus levels caused by immunoassay interference secondary to beta-galactosidase antibodies in an infected liver transplant recipient

Authors:

Knorr JP, Grewal KS, Balasubramanian M, Young N, Zaki R, Khanmoradi K, Araya V, Ortiz J.

Journal:

Pharmacotherapy (2010): 954

Three siblings with self-resolving congenital hyperthyrotropinaemia secondary to thyrotropin receptor blocking antibodies

Authors:

Azzopardi P, Forrester M, Ehtisham S.

Journal:

J Paediatr Child Health (2010): 439

Nanosilver-doped DNA polyion complex membrane for electrochemical immunoassay of carcinoembryonic antigen using nanogold-labeled secondary antibodies

Authors:

Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.

Journal:

Anal Chim Acta (2010): 126

Physical properties

Molecular weight	~150000
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.23
Correction factor (280 nm)	0.14
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.64 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet (Catalog 16460)
Safety Data Sheet (Catalog 16739)
Certificate of Analysis
Brochure: Secondary Detection Probes & Kits

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Page updated on October 29, 2025