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iFluor® Secondary Antibodies
iFluor® conjugated secondary antibodies are powerful tools that assist in identifying and visualizing target proteins in many applications, including fluorescent cell imaging, immunohistochemistry, flow cytometry, and western blotting. iFluor® conjugates are produced with the highest quality secondary antibodies and subjected to rigorous quality control assays. To ensure customers receive products with the greatest sensitivity and selectivity, iFluor® secondary antibodies have been affinity purified and cross-adsorbed to minimize cross-reactivity.
Fig. 1
iFluor546 cell stain
iFluor® cell stain
iFluor® Dyes - Superior Performance

Conjugates labeled with iFluor® dyes exhibit superior brightness, higher signal-to-noise ratios and improved photostability, outperforming spectrally similar secondary antibody conjugates such as fluorescein and most Alexa Fluor® secondary antibody conjugates. The excellent photostability and pH-insensitive fluorescence of our iFluor® secondary antibodies, allows researchers more time for capturing images (Figure 1).
Fig. 2
Photostability Comparison
Comparison of photobleaching rates of iFluor® 488 goat anti-mouse IgG (Cat No. 16735) versus FITC goat anti-mouse IgG (Cat No. 16852). Images were taken at 120-second intervals on a Keyence X710 fluorescence microscope. TFI represents total fluorescence intensity as calculated by ImageJ software.
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Document: 01.0104.220114r1
Last updated Fri Sep 12 2025

iFluor® Secondary Antibodies