iFluor® Secondary Antibodies

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iFluor® 546 cell stain

iFluor® conjugated secondary antibodies are powerful tools that assist in identifying and visualizing target proteins in many applications, including fluorescent cell imaging, immunohistochemistry, flow cytometry, and western blotting. iFluor® conjugates are produced with the highest quality secondary antibodies and subjected to rigorous quality control assays. To ensure customers receive products with the greatest sensitivity and selectivity, iFluor® secondary antibodies have been affinity purified and cross-adsorbed to minimize cross-reactivity.

iFluor Secondary Antibody Product Guide
iFluor® Dyes - Superior Performance
Product Ordering Information

iFluor Secondary Antibody Product Guide

Table 1. iFluor® Secondary Antibody Product Guide

Parameter ▲ ▼	Definition ▲ ▼
Detection Strategy	It uses indirect detection strategies whereby the secondary antibody detects an unlabeled primary antibody bound to the target of interest.
Applications	Designed for use in various cell analysis and protein analysis applications, including cell imaging (e.g., IHC/ICC/IF), fluorescent ELISA, and fluorescent western blotting.
Product Attributes	Enhanced sensitivity - multiple iFluor® secondary antibodies can bind to a single primary antibody resulting in signal amplification and increased assay sensitivity. pH Insensitivity - iFluor® dyes emit bright fluorescence intensities over a wide range (pH 3 to 11). Excellent Water Solubility - eliminates the precipitation and aggregation of iFluor® secondary antibodies. Increased flexibility when designing multi-color fluorescent western blot panels.
Targets Available	Host Goat Species Mouse Rabbit Human
Fluorophores Available	iFluor® 350 iFluor® 405 iFluor® 430 iFluor® 450 iFluor® 488 iFluor® 514 iFluor® 532 iFluor® 546 iFluor® 555 iFluor® 560 iFluor® 568 iFluor® 594 iFluor® 610 iFluor® 633 iFluor® 647 iFluor® 660 iFluor® 670 iFluor® 680 iFluor® 700 iFluor® 710 iFluor® 750 iFluor® 790 iFluor® 800 iFluor® 810 iFluor® 820 iFluor® 840 iFluor® 860 iFluor® A7

iFluor® Dyes - Superior Performance

Conjugates labeled with iFluor® dyes exhibit superior brightness, higher signal-to-noise ratios and improved photostability, outperforming spectrally similar secondary antibody conjugates such as fluorescein and most Alexa Fluor^® secondary antibody conjugates. The excellent photostability and pH-insensitive fluorescence of our iFluor® secondary antibodies, allows researchers more time for capturing images (Figure 1).

Comparison of photobleaching rates of iFluor® 488 goat anti-mouse IgG (Cat No. 16735) versus FITC goat anti-mouse IgG (Cat No. 16852). Images were taken at 120-second intervals on a Keyence X710 fluorescence microscope. TFI represents total fluorescence intensity as calculated by ImageJ software.

Table 2. iFluor® Dyes Spectral Properties

iFluor® Dye ▲ ▼	Mol. Wt. ▲ ▼	Abs. (nm) ▲ ▼	Em. (nm) ▲ ▼	Spectrum ▲ ▼	ε¹ ▲ ▼	Φ² ▲ ▼	CF at 260 nm³ ▲ ▼	CF at 280 nm⁴ ▲ ▼
iFluor® 350	749.85	345	450		20,000	0.95	0.83	0.23
iFluor® 405	755.58	403	427		37,000	0.91	0.48	0.77
iFluor® 430	688.79	433	498		40,000	0.78	0.68	0.3
iFluor® 440	692.83	434	480		40,000	N/D	0.352	0.229
iFluor® 445	968.01	446	558		N/D	N/D	N/D	N/D
iFluor® 450	603.69	451	502		40,000	0.82	0.45	0.27
iFluor® 460	793.99	468	493		80,000	0.8	0.98	0.46
iFluor® 488	945.07	491	516		75,000	0.9	0.21	0.11
iFluor® 500	981.12	501	520		N/D	N/D	N/D	N/D
iFluor® 510	951.91	511	530		N/D	N/D	N/D	N/D
iFluor® 514	1013.95	511	527		75,000	0.83	0.265	0.116
iFluor® 532	914.06	537	560		90,000	0.68	0.26	0.16
iFluor® 540	853.96	540	557		N/D	N/D	N/D	0.105
iFluor® 546	1145.41	541	557		100,000	0.67	0.25	0.15
iFluor® 555	1125.26	557	570		100,000	0.64	0.23	0.14
iFluor® 560	1257.45	560	571		120,000	0.57	0.0482	0.069
iFluor® 568	1173.46	568	587		100,000	0.57	0.34	0.15
iFluor® 570	881.07	557	570		N/D	N/D	N/D	N/D
iFluor® 594	1160.42	588	604		180,000	0.53	0.05	0.04
iFluor® 597	1058.29	598	618		100,000	0.7	N/D	0.514
iFluor® 605	1004.22	603	623		N/D	N/D	N/D	N/D
iFluor® 610	1211.44	610	628		110,000	0.85	0.32	0.49
iFluor® 620	1063.25	621	636		N/D	N/D	N/D	0.04
iFluor® 625	1141.99	624	640		N/D	N/D	N/D	N/D
iFluor® 633	1249.58	640	654		250,000	0.29	0.062	0.044
iFluor® 647	1274.66	656	670		250,000	0.25	0.03	0.03
iFluor® 660	1281.66	663	678		250,000	0.26	0.07	0.08
iFluor® 665	1210.53	667	692		N/D	N/D	N/D	N/D
iFluor® 670	923.15	671	682		200,000	0.55	0.03	0.033
iFluor® 675	1146.18	683	700		N/D	N/D	N/D	0.066
iFluor® 680	957.17	684	701		220,000	0.23	0.097	0.094
iFluor® 690	1462.75	685	704		220,000	N/D	N/D	N/D
iFluor® 700	977.16	690	713		220,000	0.23	0.09	0.04
iFluor® 710	1206.39	717	739		190,000	N/D	0.12	0.07
iFluor® 720	1089.29	716	740		140,000	0.14	N/D	N/D
iFluor® 740	1551.19	742	764		125,000	N/D	N/D	N/D
iFluor® 750	1416.83	757	779		275,000	0.12	0.044	0.039
iFluor® 770	1082.27	777	797		250,000	N/D	N/D	N/D
iFluor® 780	1526.90	784	808		250,000	N/D	N/D	N/D
iFluor® 790	1768.30	787	812		250,000	0.13	0.1	0.09
iFluor® 800	1541.91	801	820		250,000	0.11	0.03	0.08
iFluor® 810	1576.03	811	822		250,000	0.05	0.09	0.15
iFluor® 820	1585.92	822	850		250,000	N/D	0.11	0.16
iFluor® 840	1422	836	879		200,000	N/D	0.2	0.09
iFluor® 860	1571.90	853	878		250,000	N/D	0.1	0.14
iFluor® A7	1288.58	762	782		275,000	0.1	0.03	0.03
iFluor® Ultra 594	1436.72	586	601		180,000	N/D	N/D	N/D
iFluor® Ultra 647	2634.28	655	670		250,000	N/D	N/D	N/D
iFluor® Ultra 750	1426.78	749	773		250,000	N/D	N/D	N/D

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Product Ordering Information

Table 3. iFluor® Dye-Labeled Secondary Antibodies

Cat# ▲ ▼	Product Name ▲ ▼	Ex (nm) ▲ ▼	Em (nm) ▲ ▼	Size ▲ ▼
16440	iFluor® 350 goat anti-mouse IgG (H+L)	345	442	200 ug
16444	iFluor® 405 goat anti-mouse IgG (H+L)	401	420	200 ug
16448	iFluor® 488 goat anti-mouse IgG (H+L)	491	514	200 ug
16460	iFluor® 555 goat anti-mouse IgG (H+L)	552	567	200 ug
16468	iFluor® 594 goat anti-mouse IgG (H+L)	592	614	200 ug
16478	iFluor® 633 goat anti-mouse IgG (H+L)	638	655	200 ug
16482	iFluor® 647 goat anti-mouse IgG (H+L)	654	674	200 ug
16486	iFluor® 680 goat anti-mouse IgG (H+L)	682	701	200 ug
16494	iFluor® 700 goat anti-mouse IgG (H+L)	693	713	200 ug
16506	iFluor® 750 goat anti-mouse IgG (H+L)	753	779	200 ug
16507	iFluor® 790 goat anti-mouse IgG (H+L)	782	811	200 ug
16520	iFluor® 350 goat anti-mouse IgG (H+L) Cross Adsorbed	345	442	200 ug
16524	iFluor® 405 goat anti-mouse IgG (H+L) Cross Adsorbed	401	420	200 ug
16528	iFluor® 488 goat anti-mouse IgG (H+L) Cross Adsorbed	491	514	200 ug
16540	iFluor® 555 goat anti-mouse IgG (H+L) Cross Adsorbed	552	567	200 ug
16548	iFluor® 594 goat anti-mouse IgG (H+L) Cross Adsorbed	592	614	200 ug
16558	iFluor® 633 goat anti-mouse IgG (H+L) Cross Adsorbed	638	655	200 ug
16562	iFluor® 647 goat anti-mouse IgG (H+L) Cross Adsorbed	654	674	200 ug
16566	iFluor® 680 goat anti-mouse IgG (H+L) Cross Adsorbed	682	701	200 ug
16574	iFluor® 700 goat anti-mouse IgG (H+L) Cross Adsorbed	693	713	200 ug
16586	iFluor® 750 goat anti-mouse IgG (H+L) Cross Adsorbed	753	779	200 ug
16587	iFluor® 790 goat anti-mouse IgG (H+L) Cross Adsorbed	782	811	200 ug
16600	iFluor® 350 goat anti-rabbit IgG (H+L)	345	442	200 ug
16604	iFluor® 405 goat anti-rabbit IgG (H+L)	401	420	200 ug
16608	iFluor® 488 goat anti-rabbit IgG (H+L)	491	514	200 ug
16620	iFluor® 555 goat anti-rabbit IgG (H+L)	552	567	200 ug
16628	iFluor® 594 goat anti-rabbit IgG (H+L)	592	614	200 ug
16638	iFluor® 633 goat anti-rabbit IgG (H+L)	638	655	200 ug
16642	iFluor® 647 goat anti-rabbit IgG (H+L)	654	674	200 ug
16646	iFluor® 680 goat anti-rabbit IgG (H+L)	682	701	200 ug
16652	iFluor® 700 goat anti-rabbit IgG (H+L)	693	713	200 ug
16660	iFluor® 750 goat anti-rabbit IgG (H+L)	753	779	200 ug
16661	iFluor® 790 goat anti-rabbit IgG (H+L)	782	811	200 ug
16670	iFluor® 350 goat anti-rabbit IgG (H+L) Cross Adsorbed	345	442	200 ug
16674	iFluor® 405 goat anti-rabbit IgG (H+L) Cross Adsorbed	401	420	200 ug
16678	iFluor® 488 goat anti-rabbit IgG (H+L) Cross Adsorbed	491	514	200 ug
16690	iFluor® 555 goat anti-rabbit IgG (H+L) Cross Adsorbed	552	567	200 ug
16698	iFluor® 594 goat anti-rabbit IgG (H+L) Cross Adsorbed	592	614	200 ug
16704	iFluor® 633 goat anti-rabbit IgG (H+L) Cross Adsorbed	638	655	200 ug
16710	iFluor® 647 goat anti-rabbit IgG (H+L) Cross Adsorbed	654	674	200 ug
16712	iFluor® 680 goat anti-rabbit IgG (H+L) Cross Adsorbed	682	701	200 ug
16714	iFluor® 700 goat anti-rabbit IgG (H+L) Cross Adsorbed	693	713	200 ug
16720	iFluor® 750 goat anti-rabbit IgG (H+L) Cross Adsorbed	753	779	200 ug
16721	iFluor® 790 goat anti-rabbit IgG (H+L) Cross Adsorbed	782	811	200 ug

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