AAT Bioquest

iFluor® Secondary Antibodies

iFluor546 cell stain
iFluor® 546 cell stain
iFluor® conjugated secondary antibodies are powerful tools that assist in identifying and visualizing target proteins in many applications, including fluorescent cell imaging, immunohistochemistry, flow cytometry, and western blotting. iFluor® conjugates are produced with the highest quality secondary antibodies and subjected to rigorous quality control assays. To ensure customers receive products with the greatest sensitivity and selectivity, iFluor® secondary antibodies have been affinity purified and cross-adsorbed to minimize cross-reactivity.



iFluor Secondary Antibody Product Guide

Detection StrategyUtilizes indirect detection strategies whereby the secondary antibody detects an unlabeled primary antibody bound to the target of interest.
ApplicationsDesigned for use in various cell analysis and protein analysis applications, including cell imaging (e.g., IHC/ICC/IF), fluorescent ELISA, and fluorescent western blotting.
Product Attributes
  • Enhances sensitivity - multiple secondary antibodies can bind to a single primary antibody resulting in signal amplification and an increase in assay sensitivity.
  • Increases flexibility when designing multicolor fluorescent western blot panels.
Targets AvailableHost
  • Goat
Fluorophores Available
Suitable Mountants, Fixatives, and Antifade ReagentsCompatible with most standard mountants, fixatives, and antifading reagents.
Suitable CounterstainsCompatible with most organelle-specific stains such as nuclear counterstains, MitoLite™ dyes, and LysoBrite™ dyes.

iFluor® Dyes - Superior Performance

Conjugates labeled with iFluor® dyes exhibit superior brightness, higher signal-to-noise ratios and improved photostability, outperforming spectrally similar secondary antibody conjugates such as fluorescein and most Alexa Fluor® secondary antibody conjugates. The excellent photostability and pH-insensitive fluorescence of our iFluor® secondary antibodies, allows researchers more time for capturing images (Figure 1).

Photostability Comparison

Comparison of photobleaching rates of iFluor® 488 goat anti-mouse IgG (Cat No. 16735) versus FITC goat anti-mouse IgG (Cat No. 16852). Images were taken at 120-second intervals on a Keyence X710 fluorescence microscope. TFI represents total fluorescence intensity as calculated by ImageJ software.


Table 1. iFluor® Dyes Spectral Properties

iFluor® Dye
Mol. Wt.
Abs. (nm)
Em. (nm)
CF at 260 nm³
CF at 280 nm⁴
iFluor® 350749.8534545020,0000.950.830.23
iFluor® 405755.5840342737,0000.910.480.77
iFluor® 430688.7943349840,0000.780.680.3
iFluor® 440692.8343448040,000N/D0.3520.229
iFluor® 450603.6945150240,0000.820.450.27
iFluor® 460793.9946849380,0000.80.980.46
iFluor® 488945.0749151675,0000.90.210.11
iFluor® 5141013.9551152775,0000.830.2650.116
iFluor® 532914.0653756090,0000.680.260.16
iFluor® 5461145.41541557100,0000.670.250.15
iFluor® 5551125.26557570100,0000.640.230.14
iFluor® 5601257.45560571120,0000.570.04820.069
iFluor® 5681173.46568587100,0000.570.340.15
iFluor® 5941160.42588604180,0000.530.050.04
iFluor® Ultra 5941436.72586601180,000N/DN/DN/D
iFluor® 5971058.29598618100,0000.7N/D0.514
iFluor® 6101211.44610628110,0000.850.320.49
iFluor® 6331249.58640654250,0000.290.0620.044
iFluor® 6471274.66656670250,0000.250.030.03
iFluor® Ultra 6472634.28655670250,000N/DN/DN/D
iFluor® 6601281.66663678250,0000.260.070.08
iFluor® 6651210.53667692N/DN/DN/DN/D
iFluor® 670923.15671682200,0000.550.030.033
iFluor® 680957.17684701220,0000.230.0970.094
iFluor® 6901462.75685704220,000N/DN/DN/D
iFluor® 700977.16690713220,0000.230.090.04
iFluor® 7101206.39717739190,000N/D0.120.07
iFluor® 7201089.29716740140,0000.14N/DN/D
iFluor® 7401551.19742764125,000N/DN/DN/D
iFluor® 7501416.83757779275,0000.120.0440.039
iFluor® Ultra 7501426.78749773250,000N/DN/DN/D
iFluor® 7701082.27777797250,000N/DN/DN/D
iFluor® A71288.58762782275,0000.10.030.03
iFluor® 7801526.90784808250,000N/DN/DN/D
iFluor® 7901768.30787812250,0000.130.10.09
iFluor® 8001541.91801820250,0000.110.030.08
iFluor® 8101576.03811822250,0000.050.090.15
iFluor® 8201585.92822850250,000N/D0.110.16
iFluor® 8401422836879200,000N/D0.20.09
iFluor® 8601571.90853878250,000N/D0.10.14

Product Ordering Information

Table 2. iFluor® Dye-Labeled Secondary Antibodies

Product Name
Ex (nm)
Em (nm)
16440iFluor® 350 goat anti-mouse IgG (H+L)345442200 ug
16444iFluor® 405 goat anti-mouse IgG (H+L) 401420200 ug
16448iFluor® 488 goat anti-mouse IgG (H+L) 491514200 ug
16460iFluor® 555 goat anti-mouse IgG (H+L) 552567200 ug
16468iFluor® 594 goat anti-mouse IgG (H+L) 592614200 ug
16478iFluor® 633 goat anti-mouse IgG (H+L) 638655200 ug
16482iFluor® 647 goat anti-mouse IgG (H+L) 654674200 ug
16486iFluor® 680 goat anti-mouse IgG (H+L) 682701200 ug
16494iFluor® 700 goat anti-mouse IgG (H+L) 693713200 ug
16506iFluor® 750 goat anti-mouse IgG (H+L) 753779200 ug
16507iFluor® 790 goat anti-mouse IgG (H+L) 782811200 ug
16520iFluor® 350 goat anti-mouse IgG (H+L) *Cross Adsorbed* 345442200 ug
16524iFluor® 405 goat anti-mouse IgG (H+L) *Cross Adsorbed* 401420200 ug
16528iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed* 491514200 ug
16540iFluor® 555 goat anti-mouse IgG (H+L) *Cross Adsorbed* 552567200 ug
16548iFluor® 594 goat anti-mouse IgG (H+L) *Cross Adsorbed* 592614200 ug
16558iFluor® 633 goat anti-mouse IgG (H+L) *Cross Adsorbed* 638655200 ug
16562iFluor® 647 goat anti-mouse IgG (H+L) *Cross Adsorbed* 654674200 ug
16566iFluor® 680 goat anti-mouse IgG (H+L) *Cross Adsorbed* 682701200 ug
16574iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed* 693713200 ug
16586iFluor® 750 goat anti-mouse IgG (H+L) *Cross Adsorbed* 753779200 ug
16587iFluor® 790 goat anti-mouse IgG (H+L) *Cross Adsorbed* 782811200 ug
16600iFluor® 350 goat anti-rabbit IgG (H+L) 345442200 ug
16604iFluor® 405 goat anti-rabbit IgG (H+L) 401420200 ug
16608iFluor® 488 goat anti-rabbit IgG (H+L) 491514200 ug
16620iFluor® 555 goat anti-rabbit IgG (H+L) 552567200 ug
16628iFluor® 594 goat anti-rabbit IgG (H+L) 592614200 ug
16638iFluor® 633 goat anti-rabbit IgG (H+L) 638655200 ug
16642iFluor® 647 goat anti-rabbit IgG (H+L) 654674200 ug
16646iFluor® 680 goat anti-rabbit IgG (H+L) 682701200 ug
16652iFluor® 700 goat anti-rabbit IgG (H+L) 693713200 ug
16660iFluor® 750 goat anti-rabbit IgG (H+L) 753779200 ug
16661iFluor® 790 goat anti-rabbit IgG (H+L) 782811200 ug
16670iFluor® 350 goat anti-rabbit IgG (H+L) *Cross Adsorbed* 345442200 ug
16674iFluor® 405 goat anti-rabbit IgG (H+L) *Cross Adsorbed* 401420200 ug
16678iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed* 491514200 ug
16690iFluor® 555 goat anti-rabbit IgG (H+L) *Cross Adsorbed* 552567200 ug
16698iFluor® 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed* 592614200 ug
16704iFluor® 633 goat anti-rabbit IgG (H+L) *Cross Adsorbed* 638655200 ug
16710iFluor® 647 goat anti-rabbit IgG (H+L) *Cross Adsorbed* 654674200 ug
16712iFluor® 680 goat anti-rabbit IgG (H+L) *Cross Adsorbed* 682701200 ug
16714iFluor® 700 goat anti-rabbit IgG (H+L) *Cross Adsorbed* 693713200 ug
16720iFluor® 750 goat anti-rabbit IgG (H+L) *Cross Adsorbed* 753779200 ug
16721iFluor® 790 goat anti-rabbit IgG (H+L) *Cross Adsorbed* 782811200 ug