iFluor® Secondary Antibodies

iFluor® conjugated secondary antibodies are powerful tools that assist in identifying and visualizing target proteins in many applications, including fluorescent cell imaging, immunohistochemistry, flow cytometry, and western blotting. iFluor® conjugates are produced with the highest quality secondary antibodies and subjected to rigorous quality control assays. To ensure customers receive products with the greatest sensitivity and selectivity, iFluor® secondary antibodies have been affinity purified and cross-adsorbed to minimize cross-reactivity.

iFluor Secondary Antibody Product Guide

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Parameter	Definition
Detection Strategy	Utilizes indirect detection strategies whereby the secondary antibody detects an unlabeled primary antibody bound to the target of interest.
Applications	Designed for use in various cell analysis and protein analysis applications, including cell imaging (e.g., IHC/ICC/IF), fluorescent ELISA, and fluorescent western blotting.
Product Attributes	Enhances sensitivity - multiple secondary antibodies can bind to a single primary antibody resulting in signal amplification and an increase in assay sensitivity. Increases flexibility when designing multicolor fluorescent western blot panels.
Targets Available	Host Goat	Species Mouse Rabbit Human
Fluorophores Available	iFluor® 350 iFluor® 405 iFluor® 430 iFluor® 450 iFluor® 488 iFluor® 514 iFluor® 532 iFluor® 546 iFluor® 555 iFluor® 560	iFluor® 568 iFluor® 594 iFluor® 610 iFluor® 633 iFluor® 647 iFluor® 660 iFluor® 670 iFluor® 680 iFluor® 700 iFluor® 710	iFluor® 750 iFluor® 790 iFluor® 800 iFluor® 810 iFluor® 820 iFluor® 840 iFluor® 860 iFluor® A7
Suitable Mountants, Fixatives, and Antifade Reagents	Compatible with most standard mountants, fixatives, and antifading reagents.
Suitable Counterstains	Compatible with most organelle-specific stains such as nuclear counterstains, MitoLite™ dyes, and LysoBrite™ dyes.

iFluor® Dyes - Superior Performance

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Conjugates labeled with iFluor® dyes exhibit superior brightness, higher signal-to-noise ratios and improved photostability, outperforming spectrally similar secondary antibody conjugates such as fluorescein and most Alexa Fluor^® secondary antibody conjugates. The excellent photostability and pH-insensitive fluorescence of our iFluor® secondary antibodies, allows researchers more time for capturing images (Figure 1).