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iFluor® 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 594 goat anti-rabbit IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~592 nm and ~614 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 594 goat anti-rabbit IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 594 goat anti-mouse IgG (H+L)58760320000010.5310.050.04
iFluor® 594 goat anti-mouse IgG (H+L) *Cross Adsorbed*58760320000010.5310.050.04
iFluor® 350 goat anti-rabbit IgG (H+L)3454502000010.9510.830.23
iFluor® 405 goat anti-rabbit IgG (H+L)4034273700010.9110.480.77
iFluor® 488 goat anti-rabbit IgG (H+L)4915167500010.910.210.11
iFluor® 514 goat anti-rabbit IgG (H+L)5115277500010.8310.2650.116
iFluor® 532 goat anti-rabbit IgG (H+L)5375609000010.6810.260.16
iFluor® 555 goat anti-rabbit IgG (H+L)55757010000010.6410.230.14
iFluor® 633 goat anti-rabbit IgG (H+L)64065425000010.2910.0620.044
iFluor® 647 goat anti-rabbit IgG (H+L)65667025000010.2510.030.03
iFluor® 680 goat anti-rabbit IgG (H+L)68470122000010.2310.0970.094
iFluor® 700 goat anti-rabbit IgG (H+L)69071322000010.2310.090.04
iFluor® 750 goat anti-rabbit IgG (H+L)75777927500010.1210.0440.039
iFluor® 790 goat anti-rabbit IgG (H+L)78781225000010.1310.10.09
iFluor® 350 goat anti-rabbit IgG (H+L) *Cross Adsorbed*3454502000010.9510.830.23
iFluor® 405 goat anti-rabbit IgG (H+L) *Cross Adsorbed*4034273700010.9110.480.77
iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*4915167500010.910.210.11
iFluor® 514 goat anti-rabbit IgG (H+L) *Cross Adsorbed*5115277500010.8310.2650.116
iFluor® 532 goat anti-rabbit IgG (H+L) *Cross Adsorbed*5375609000010.6810.260.16
iFluor® 555 goat anti-rabbit IgG (H+L) *Cross Adsorbed*55757010000010.6410.230.14
iFluor® 633 goat anti-rabbit IgG (H+L) *Cross Adsorbed*64065425000010.2910.0620.044
iFluor® 647 goat anti-rabbit IgG (H+L) *Cross Adsorbed*65667025000010.2510.030.03
iFluor® 680 goat anti-rabbit IgG (H+L) *Cross Adsorbed*68470122000010.2310.0970.094
iFluor® 700 goat anti-rabbit IgG (H+L) *Cross Adsorbed*69071322000010.2310.090.04
iFluor® 750 goat anti-rabbit IgG (H+L) *Cross Adsorbed*75777927500010.1210.0440.039
iFluor® 790 goat anti-rabbit IgG (H+L) *Cross Adsorbed*78781225000010.1310.10.09
iFluor® 546 goat anti-rabbit IgG (H+L)54155710000010.6710.250.15
iFluor® 546 goat anti-rabbit IgG (H+L) *Cross Adsorbed*54155710000010.6710.250.15
iFluor® 568 goat anti-rabbit IgG (H+L)56858710000010.5710.340.15
iFluor® 568 goat anti-rabbit IgG (H+L) *Cross Adsorbed*56858710000010.5710.340.15
iFluor® 800 goat anti-rabbit IgG (H+L)80182025000010.1110.030.08
iFluor® 800 goat anti-rabbit IgG (H+L) *Cross Adsorbed*80182025000010.1110.030.08
iFluor® 810 goat anti-rabbit IgG (H+L)81182225000010.0510.090.15
iFluor® 810 goat anti-rabbit IgG (H+L) *Cross Adsorbed*81182225000010.0510.090.15
iFluor® 820 goat anti-rabbit IgG (H+L)82285025000010.110.16
iFluor® 820 goat anti-rabbit IgG (H+L) *Cross Adsorbed*82285025000010.110.16
iFluor® 840 goat anti-rabbit IgG (H+L)8368792000001-0.20.09
iFluor® 840 goat anti-rabbit IgG (H+L) *Cross Adsorbed*8368792000001-0.20.09
iFluor® 860 goat anti-rabbit IgG (H+L)85387825000010.10.14
iFluor® 860 goat anti-rabbit IgG (H+L) *Cross Adsorbed*85387825000010.10.14
Show More (31)

Citations

View all 14 citations: Citation Explorer
Extracellular vesicles from alveolar macrophages harboring phagocytosed methicillin-resistant Staphylococcus aureus induce necroptosis
Authors: Bai, Songjie and Wen, Xuehuan and Li, Bingyu and Hu, Ruomeng and Yang, Jie and Yu, Qing and Zeng, Xianchang and Feng, Huajun and Zhu, Feng and Cai, Zhijian and others,
Journal: Cell Reports (2024)
IL-37 protects against house dust mite-induced airway inflammation and airway epithelial barrier dysfunction via inhibiting store-operated calcium entry
Authors: Wang, Changchang and Zhong, Jian and Hu, Jiaqian and Cao, Can and Qi, Shiquan and Ma, Ruxue and Fu, Wei and Zhang, Xiaolian and Akdis, Cezmi A and Gao, Yadong
Journal: International Immunopharmacology (2024): 112525
hnRNPH1 maintains mitochondrial homeostasis by establishing NRF1/DRP1 retrograde signaling under mitochondrial stress
Authors: Zhao, Lili and Zou, Xiaotian and Deng, Jiaqiang and Sun, Bin and Li, Yan and Zhao, Li and Zhao, Hong and Zhang, Xiao and Yuan, Xieyong and Zhao, Xudong and others,
Journal: Cell Death \& Differentiation (2024): 1--16
Angiotension II directly bind P2X7 receptor to induce myocardial ferroptosis and remodeling by activating human antigen R
Authors: Zhong, Xin and Wang, Kangwei and Wang, Yonghua and Wang, Luya and Wang, Sudan and Huang, Weijian and Jia, Zhuyin and Dai, Shan-Shan and Huang, Zhouqing
Journal: Redox Biology (2024): 103154

References

View all 191 references: Citation Explorer
Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668
Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728
Falsely elevated tacrolimus levels caused by immunoassay interference secondary to beta-galactosidase antibodies in an infected liver transplant recipient
Authors: Knorr JP, Grewal KS, Balasubramanian M, Young N, Zaki R, Khanmoradi K, Araya V, Ortiz J.
Journal: Pharmacotherapy (2010): 954
Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency
Authors: Buckova A., undefined
Journal: Epidemiol Mikrobiol Imunol (2010): 133
Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis
Authors: Boonrod K, Roth B, Leong Ngar S, Krczal G.
Journal: Anal Biochem (2010): 124
Page updated on September 18, 2024

Ordering information

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Unit size
200 ug
1 mg
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Physical properties

Molecular weight

~150000

Solvent

Water

Spectral properties

Absorbance (nm)

587

Correction Factor (260 nm)

0.05

Correction Factor (280 nm)

0.04

Extinction coefficient (cm -1 M -1)

2000001

Excitation (nm)

587

Emission (nm)

603

Quantum yield

0.531

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
HeLa cells were stained with rabbit anti-tubulin followed with iFluor<sup>TM</sup>&nbsp;594 goat anti-rabbit IgG (H+L) (red); actin filaments were stained with Phalloidin-iFluor<sup>TM</sup>&nbsp;488 conjugate (green); and nuclei were stained with DAPI (blue).
HeLa cells were stained with rabbit anti-tubulin followed with iFluor<sup>TM</sup>&nbsp;594 goat anti-rabbit IgG (H+L) (red); actin filaments were stained with Phalloidin-iFluor<sup>TM</sup>&nbsp;488 conjugate (green); and nuclei were stained with DAPI (blue).
HeLa cells were stained with rabbit anti-tubulin followed with iFluor<sup>TM</sup>&nbsp;594 goat anti-rabbit IgG (H+L) (red); actin filaments were stained with Phalloidin-iFluor<sup>TM</sup>&nbsp;488 conjugate (green); and nuclei were stained with DAPI (blue).