iFluor® 488 goat anti-rabbit IgG (H+L)

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

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Physical properties

Molecular weight	~150000
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*

Overview SDS Protocol

Molecular weight

~150000

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.11

Extinction coefficient (cm ^-1 M ^-1)

75000¹

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.9¹

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 488 goat anti-rabbit IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~491 nm and ~514 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 488 goat anti-rabbit IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 488 goat anti-mouse IgG (H+L)	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 goat anti-mouse IgG (H+L) Cross Adsorbed	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 350 goat anti-rabbit IgG (H+L)	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 405 goat anti-rabbit IgG (H+L)	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 514 goat anti-rabbit IgG (H+L)	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 532 goat anti-rabbit IgG (H+L)	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 555 goat anti-rabbit IgG (H+L)	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 594 goat anti-rabbit IgG (H+L)	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 633 goat anti-rabbit IgG (H+L)	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 647 goat anti-rabbit IgG (H+L)	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 goat anti-rabbit IgG (H+L)	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 goat anti-rabbit IgG (H+L)	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 goat anti-rabbit IgG (H+L)	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 goat anti-rabbit IgG (H+L)	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 350 goat anti-rabbit IgG (H+L) Cross Adsorbed	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 405 goat anti-rabbit IgG (H+L) Cross Adsorbed	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 514 goat anti-rabbit IgG (H+L) Cross Adsorbed	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 532 goat anti-rabbit IgG (H+L) Cross Adsorbed	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 555 goat anti-rabbit IgG (H+L) Cross Adsorbed	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 594 goat anti-rabbit IgG (H+L) Cross Adsorbed	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 633 goat anti-rabbit IgG (H+L) Cross Adsorbed	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 647 goat anti-rabbit IgG (H+L) Cross Adsorbed	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 goat anti-rabbit IgG (H+L) Cross Adsorbed	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 goat anti-rabbit IgG (H+L) Cross Adsorbed	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 goat anti-rabbit IgG (H+L) Cross Adsorbed	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 goat anti-rabbit IgG (H+L) Cross Adsorbed	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 546 goat anti-rabbit IgG (H+L)	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 546 goat anti-rabbit IgG (H+L) Cross Adsorbed	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 568 goat anti-rabbit IgG (H+L)	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 568 goat anti-rabbit IgG (H+L) Cross Adsorbed	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 800 goat anti-rabbit IgG (H+L)	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 800 goat anti-rabbit IgG (H+L) Cross Adsorbed	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 810 goat anti-rabbit IgG (H+L)	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 810 goat anti-rabbit IgG (H+L) Cross Adsorbed	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 820 goat anti-rabbit IgG (H+L)	822	850	250000¹		0.11	0.16
iFluor® 820 goat anti-rabbit IgG (H+L) Cross Adsorbed	822	850	250000¹		0.11	0.16
iFluor® 840 goat anti-rabbit IgG (H+L)	836	879	200000¹	-	0.2	0.09
iFluor® 840 goat anti-rabbit IgG (H+L) Cross Adsorbed	836	879	200000¹	-	0.2	0.09
iFluor® 860 goat anti-rabbit IgG (H+L)	853	878	250000¹		0.1	0.14
iFluor® 860 goat anti-rabbit IgG (H+L) Cross Adsorbed	853	878	250000¹		0.1	0.14
Show More (31)

Images

Figure 1. HeLa cells were stained with rabbit anti-tubulin followed by iFluor 488 goat anti-rabbit IgG (H+L), and nuclei were stained with Nuclear Red DCS1.

Citations

View all 20 citations: Citation Explorer

The inhibitory effects of lobaplatin, or in combination with gemcitabine on triple-negative breast cancer cells in vitro and in vivo
Authors: Jiang, Chengyan and Zhang, Ye and Xu, Xiaoyu and Su, Shanshan and Pan, Huafeng and Jiang, Aiqin
Journal: Oncologie (2023)

The roles and molecular mechanisms of long non-coding RNA WT1-AS in the maintenance and development of gastric cancer stem cells
Authors: Zhang, Xiaobei and Jin, Meng and Liu, Shiqi and Zang, Mingde and Hu, Lei and Du, Tao and Zhang, Baogui
Journal: Heliyon (2023)

Rab11-FIP4 interacts with ARF5 to promote cancer stemness in hepatocellular carcinoma
Authors: Song, Feifeng and Zhang, Qi and Lu, Xixuan and Xu, Tong and Hu, Qing and Hu, Xiaoping and Fan, Weijiao and Zhang, Yiwen and Huang, Ping
Journal: Journal of Physiology and Biochemistry (2023): 1--14

Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone
Authors: Wang, Chong and Xie, Linding and Xing, Yien and Liu, Min and Yang, Jun and Gao, Nannan and Cai, Yihong
Journal: PLOS Neglected Tropical Diseases (2023): e0011607

Combination of Zearalenone and Deoxynivalenol Induces Apoptosis by Mitochondrial Pathway in Piglet Sertoli Cells: Role of Endoplasmic Reticulum Stress
Authors: Hai, Sirao and Chen, Jiawen and Ma, Li and Wang, Chenlong and Chen, Chuangjiang and Rahman, Sajid Ur and Zhao, Chang and Feng, Shibin and Wu, Jinjie and Wang, Xichun
Journal: Toxins (2023): 471

Nanoscintillator-Mediated X-Ray-Triggered Boosting Transformation of Fe3+ into Fe2+ for Enhancing Tumor Ferroptosis/Immunotherapy
Authors: Zhang, Caiju and Lu, Shuting and Deng, Kai and Qian, Wang and Liu, Yue and Li, Yi and Jin, Shiqi and Suo, Ruiyang and Xu, Haibo and Wu, Bo
Journal: Advanced Functional Materials (2023): 2301462

Ferritin was involved in interleukin-17A enhanced osteogenesis through autophagy activation
Authors: Yuan, Wenlin and Yang, Yuting and Wei, Yingming and Yu, Xufei and Bao, Jiaqi and Zhong, Jiahui and Wang, Zhongxiu and Chen, Lili
Journal: International Immunopharmacology (2023): 110916

Inducible co-stimulator inhibits lipid phagocytosis of human aortic smooth muscle cells by down-regulating CD36 expression
Authors: Liang, Meng and Guo, Xuemin and Wu, Heming and Zhong, Zhixiong
Journal: Journal of Thoracic Disease (2022): 147

Four-Octyl Itaconate Attenuates UVB-Induced Melanocytes and Keratinocytes Apoptosis by Nrf2 Activation-Dependent ROS Inhibition
Authors: Xie, Yongyi and Chen, Zile and Wu, Zhouwei
Journal: Oxidative Medicine and Cellular Longevity (2022)

The Bioactive Phenolic Agents Diaryl Ether CVB2-61 and Diarylheptanoid CVB4-57 as Connexin Hemichannel Blocker
Authors: Dierks, Anne and Vanucci-Bacqu{\'e}, Corinne and Sch{\"a}fer, Anne-Marie and Lehrich, Tina and Ruhe, Frederike and Schadzek, Patrik and Bedos-Belval, Florence and Ngezahayo, Anaclet
Journal: (2022)

References

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