iFluor® 488 goat anti-rabbit IgG (H+L)
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~150000 |
Solvent | Water |
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed* |
Overview | ![]() ![]() |
See also: Anti-Rabbit Secondary Antibodies, Antibodies and Proteomics, Antibody and Protein Labeling, Bioconjugation, iFluor® Dyes and Kits, iFluor Secondary Reagents, Immunohistochemistry (IHC), Secondary Antibodies, Western Blotting Assays
Molecular weight ~150000 | Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.91 |
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 488 goat anti-rabbit IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~491 nm and ~514 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 488 goat anti-rabbit IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 488 goat anti-mouse IgG (H+L) | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 350 goat anti-rabbit IgG (H+L) | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 405 goat anti-rabbit IgG (H+L) | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
iFluor® 514 goat anti-rabbit IgG (H+L) | 511 | 527 | 750001 | 0.831 | 0.265 | 0.116 |
iFluor® 532 goat anti-rabbit IgG (H+L) | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
iFluor® 555 goat anti-rabbit IgG (H+L) | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 594 goat anti-rabbit IgG (H+L) | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
iFluor® 633 goat anti-rabbit IgG (H+L) | 640 | 654 | 2500001 | 0.291 | 0.062 | 0.044 |
Show More (31) |
Images
Citations
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Inducible co-stimulator inhibits lipid phagocytosis of human aortic smooth muscle cells by down-regulating CD36 expression
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Four-Octyl Itaconate Attenuates UVB-Induced Melanocytes and Keratinocytes Apoptosis by Nrf2 Activation-Dependent ROS Inhibition
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Journal: Oxidative Medicine and Cellular Longevity (2022)
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The Bioactive Phenolic Agents Diaryl Ether CVB2-61 and Diarylheptanoid CVB4-57 as Connexin Hemichannel Blocker
Authors: Dierks, Anne and Vanucci-Bacqu{\'e}, Corinne and Sch{\"a}fer, Anne-Marie and Lehrich, Tina and Ruhe, Frederike and Schadzek, Patrik and Bedos-Belval, Florence and Ngezahayo, Anaclet
Journal: (2022)
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Journal: (2022)
Investigations into the effects and related upstream/downstream regulatory mechanisms of long non-coding RNA WT1-AS in the maintenance and development of gastric cancer stem cells in vitro and in vivo
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IL-17A-producing T cells exacerbate fine particulate matter-induced lung inflammation and fibrosis by inhibiting PI3K/Akt/mTOR-mediated autophagy
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Journal: Journal of cellular and molecular medicine (2020): 8532--8544
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Authors: Dilger, Nadine and Neehus, Anna-Lena and Grieger, Klaudia and Hoffmann, Andrea and Menssen, Max and Ngezahayo, Anaclet
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Authors: Dilger, Nadine and Neehus, Anna-Lena and Grieger, Klaudia and Hoffmann, Andrea and Menssen, Max and Ngezahayo, Anaclet
Journal: Frontiers in cell and developmental biology (2020): 869
MRGPRX4 is a bile acid receptor for human cholestatic itch
Authors: Yu, Huasheng and Zhao, Tianjun and Liu, Simin and Wu, Qinxue and Johnson, Omar and Wu, Zhaofa and Zhuang, Zihao and Shi, Yaocheng and Peng, Luxin and He, Renxi and others, undefined
Journal: eLife (2019): e48431
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Stimulation of the A
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{\.I}nd{\"u}klenmi{\c{s}} pluripotent k{\"o}k h{\"u}cre tabanl{\i} multiple skleroz modelinde oligodendrositlere {\"o}zg{\"u} fenotiplerin ara{\c{s}}t{\i}r{\i}lmas{\i}
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References
View all 191 references: Citation Explorer
Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668
Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728
Falsely elevated tacrolimus levels caused by immunoassay interference secondary to beta-galactosidase antibodies in an infected liver transplant recipient
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Journal: Pharmacotherapy (2010): 954
Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency
Authors: Buckova A., undefined
Journal: Epidemiol Mikrobiol Imunol (2010): 133
Authors: Buckova A., undefined
Journal: Epidemiol Mikrobiol Imunol (2010): 133
Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis
Authors: Boonrod K, Roth B, Leong Ngar S, Krczal G.
Journal: Anal Biochem (2010): 124
Authors: Boonrod K, Roth B, Leong Ngar S, Krczal G.
Journal: Anal Biochem (2010): 124
Nanosilver-doped DNA polyion complex membrane for electrochemical immunoassay of carcinoembryonic antigen using nanogold-labeled secondary antibodies
Authors: Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.
Journal: Anal Chim Acta (2010): 126
Authors: Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.
Journal: Anal Chim Acta (2010): 126
Three siblings with self-resolving congenital hyperthyrotropinaemia secondary to thyrotropin receptor blocking antibodies
Authors: Azzopardi P, Forrester M, Ehtisham S.
Journal: J Paediatr Child Health (2010): 439
Authors: Azzopardi P, Forrester M, Ehtisham S.
Journal: J Paediatr Child Health (2010): 439
Double-blotting: a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures
Authors: Lasne F., undefined
Journal: Methods Mol Biol (2009): 213
Authors: Lasne F., undefined
Journal: Methods Mol Biol (2009): 213
Parkinsonism/dystonia syndrome secondary to multiple sclerosis with anti-basal-ganglia antibodies
Authors: Delgado S, Baez S, Singer C, Sengun C, Sheremata WA, Papapetropoulos S.
Journal: Mov Disord (2009): 309
Authors: Delgado S, Baez S, Singer C, Sengun C, Sheremata WA, Papapetropoulos S.
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Neutralizing antibodies and secondary therapy failure after treatment with botulinum toxin type A: much ado about nothing
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Journal: Clin Neuropharmacol (2009): 213
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Journal: Clin Neuropharmacol (2009): 213